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We investigated the association between 157 SNPs located in 75 candidate genes involved in the immune system and proxy traits for resistance to gastrointestinal nematodes in sheep. A total of 211 lambs from eight flocks were sampled. Nematode eggs per gram were counted and classified as: (i) Strongyles, (ii) Nematodirus spp., (iii) Trichuris spp. and (iv) Marshallagia marshalli. Single- and multiple-locus models were used to test the marker-trait associations. Seven significant SNPs were identified on chromosomes OAR6, 15, 16, and 19. These findings provide insights for breeding nemarode-resistant traits in low-input production systems. General linear model, fixed and random model circulating probability unification, and Bayesian-information and linkage-disequilibrium iteratively nested keyway analyses identified a significant association between the eggs per gram of Strongyles nematodes and a specific variant of the PRLR gene.

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Avian coccidiosis causes inflammation and decreases growth performance in poultry. Arginine alleviates inflammation and improves growth performance. This study aimed to evaluate the effects of different levels of arginine on growth performance, lipid profile, and inflammatory responses in broiler chicks challenged with Eimeria spp. A total of 384 one-d-old broiler chicks were assigned into eight groups and six replications per group. At 21 days of age, a mixture of Eimeria species was used for induction of challenge in the broiler chicks. Broiler chicks were divided into infected and uninfected groups and received arginine at 85, 100, 125, and 150 % of recommended level. Growth performance, serum nitric oxide (NO), pro-inflammatory cytokines, lipid profile, and faecal oocyst were further assessed. Broiler chicks challenged with coccidiosis showed lower growth performance compared to non-challenged broiler chicks in grower and finisher periods; however, those fed diets containing 125 and 150 % arginine showed better growth performance compared to broiler chicks fed with 85 and100 % levels. The challenged broiler chicks showed higher concentrations for serum NO, lipid profile, and pro-inflammatory cytokines in comparison to uninfected ones. Nevertheless, adding arginine into diet at 125 and 150 % levels increased the levels of serum NO and decreased the concentration of pro-inflammatory cytokines. In conclusion, higher levels of arginine caused anti-inflammatory effects in broiler chicks challenged with coccidiosis.

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During March 2012 through February 2013, 100 freshwater ornamental fishes in 22 species from some aquarium fish shops were examined. Specimens were dissected and tissue samples consisted of liver, kidney, spleen, heart, intestine, ovary, brain and eye were fixed in 10 % buffered formalin and sections were provided and stained with hematoxylin and eosin, Periodic Acid-Schiff, Giemsa and acid-fast staining (Ziehl-Neelsen). At present study six species of protozoans consisting of Eimeria spp. Cryptosporidium spp., Tetrahymena corlissi, Thecamoeba spp., Giardia spp., Myxobolus spp. and two metazoan parasite consisting of Nematoda spp. and Benedenia monticelli were identified. Thecamoeba, B. monticelli and Cryptosporidium spp. were not reported in previous Iranian studies and it is the first report of infestation to this parasite in ornamental fish in Iran.

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Hydatidosis is a widespread zoonotic disease with a worldwide distribution. This disease produces significant financial losses and it remains a serious health problem in a number of countries. Hydatidosis results from ingesting the eggs of Echinococcus granulosus, and the developing larvae envelop themselves in a cyst which forms in the organs of the intermediate hosts. The aim of this study was to investigate the prevalence of cystic echinococcosis in sheep, cattle, buffalo, and goats, slaughtered in the Tabriz Abattoir, Northwest of Iran. From April 2012 to April 2013, out of 14828 slaughtered animals examined for CE (hydatidosis) in liver and lungs (5000 sheep, 6125 cattle, 1103 buffaloes and 2600 goats), 25.57% were infected. The rates of CE in lungs and liver were 15.2% and 7.18% in sheep, respectively, 15.30% and 9.73% in goats, respectively, 18.71% and 9.61% in cattle, respectively and 15.68% and 11.24% in buffaloes, respectively. The infection rate was higher in lungs and was more pronounced in cattle (P<0.05). There were significant differences found in the prevalence rates of the various ruminants, and also between the sexes (P<0.05). The findings of the present study revealed that lungs were the main infected tissue, and male animals were less likely to be infected than female animals. The infection rates of the different ruminants increased significantly with age, (P<0.05).

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OBJECTIVE: To determine Neospora caninum (N. caninum) as a cause of bovine abortion in dairy cows by ELISA, PCR and Pathological methods in Tabriz, Northwest of Iran. METHODS: For study of outbreak of neosporosis, blood samples were collected from 76 Holstein aborted dairy cows in Tabriz (Northwest Iran). Antibodies to N. caninum were assayed by using a commercially ELISA kit (IDEXX, USA). IgG against N. caninum were found in 14 (18.4%) cases. Aborted fetuses of these seropositive dams were proposed for histopathological and molecular investigations. Brains, spinal cords and placentas of the fetuses were fixed in 10% buffered formalin for histopathology. Also 5-10 g of brain tissue was sampled for DNA extraction. In 6 out of 14 (42.8%) fetuses, brain tissue was positive in PCR. All dams of these fetuses were serologically seropositive in ELISA test. RESULTS: Histopathologically, the lesions consistent with N. caninum were observed in brains, spinal cords and placenta of all fetuses. Lesions in CNS included severe congestion, perivascular and perineuronal edema, status spongiosis, perivascular cuffing, focal gliosis, nourophagy and focal necrosis. There were some Neospora-like cysts in brain. In placentas, severe congestion, perivascular infiltration of mononuclear cells, vascular thrombosis, focal placentitis and necrotic foci in cotyledons were noticed. CONCLUSIONS: The results of present study agreed the results of similar studies about serological, histopathological and molecular results of other studies about neosporosis and it seems to support the outbreak of N. caninum-associated abortion in dairy cows in Tabriz (Northwest Iran).

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During 1 year (April 2009-April 2010), the prevalence of Linguatula serrata (L. serrata) nymphs in liver and mesenteric and mediastinal lymph nodes (MLN) of 740 native goats of different sex and ages were examined in Tabriz, north west of Iran. Initially the organs were macroscopically examined for nymphal stage of L. serrata and then were digested by acid-pepsin method to further investigation. The prevalence rate was obtained 55.27%. The mesenteric lymph nodes (MLN) in 334 (45.13%), the lymph nodes (LN) in 102 (13.78%) and the livers in 8 (1.08%) goats out of 740 were infected with L. serrata nymphs. The results indicated a high rate of infection of LN in goats in this region of Iran.

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BACKGROUND: The aim of this study was to determine the seroprevalence of antibody to Neospora caninum in healthy and aborted dairy cattle in Tabriz, capital of East-Azarbaijan in northwest of Iran. METHODS: In this cross-sectional study serum samples were collected from 266 healthy and aborted Holestein-Feriesisnc cows from September 2008 to August 2009. The sera were analyzed to detect of antibody against N. caninum using the commercially ELISA kit. RESULTS: Seroprevalence of antibody to N. caninum was 10.5% in Tabriz dairy cattle. Also the abortion rate in all cattle sampled was 33.6% but percentage of seropositive aborted cattle was 18.4%. CONCLUSION: Neosporosis could be one of the possible causes of abortion in dairy cattle in Tabriz and regarding the distribution in dogs as definitive host for the parasite, further studies in dog and cattle are recommended.

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Three different suture patterns (simple interrupted, interrupted horizontal mattress, subcuticular) were placed in a full thickness incision (skin and body wall) of 18 goldfish (Carassius auratus). After 14 days all fish were euthanized using benzocaine solution. The tissue reactions were evaluated by gross visual inspection and histopathological examination. The superficial inflammatory reactions were graded on a scale from 0 (no inflammation) to 3 (severe inflammation). The inflammatory response in histological examination was graded on a scale from 0 (no inflammatory response or normal skin tissue) to 5 (severe inflammatory response and necrosis). The interrupted horizontal mattress induced a moderately severe to severe inflammatory response and necrosis (grades 4-5) but the subcuticular suture induced a very mild to mild inflammatory response (grades 0-1). The simple interrupted suture induced a moderate to moderately severe inflammatory response (grades 2-4). In conclusion, results showed that a subcuticular suture is the most appropriate to use in the closure of a full thickness body wall incision.

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OBJECTIVES: Carpal tunnel syndrome (CTS) is the most common neuropathy that can be diagnosed with confidence by the nerve conduction study (NCS). One of the recent treatments of CTS is the application of low power laser (LPL) therapy. The present study evaluates the effects of LPL irradiation through NCS and clinical signs and symptoms. METHODS: A total of 80 patients were included in this study. Diagnosis of CTS was based on both clinical examination and electromyographic (EMG) findings. Patients were randomly assigned into two groups. Test group (group A) underwent laser therapy (9-11 joules/cm2) over the carpal tunnel area. Control group (group B) received sham laser therapy. Pain, hand grip strength, median proximal sensory and motor latencies, transcarpal median sensory nerve conduction (SNCV) were recorded. After fifteen sessions of irradiation (five times per week), parameters were recorded again and clinical symptoms were measured in both groups. Pain was evaluated by Visual Analog Scale (VAS; day-night). Hand grip was measured by Jamar dynometer. Paired t-test and independent sample t-test were used for statistical analysis. RESULTS: There was a significant improvement in clinical symptoms and hand grip in group A (p < 0.001). Proximal median sensory latency, distal median motor latency and median sensory latencies were significantly decreased (p < 0.001). Transcarpal median SNCV increased significantly after laser irradiation (p < 0.001). There were no significant changes in group B except changes in clinical symptoms (p < 0.001). CONCLUSIONS: Laser therapy as a new conservative treatment is effective in treating CTS paresthesia and numbness and improves the subjects' power of hand grip and electrophysiological parameters.

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Flavobacterium psychrophilum is the causative agent of bacterial cold water disease and rainbow trout fry syndrome, disease entities responsible for substantial economic losses in salmonid aquaculture. Problems associated with epizootics include high mortality rate, increased susceptibility to other diseases, high labour costs of treatment and the enormous expenditure on chemotherapy. Despite the increasing significance of the disease, the pathogenesis of F. psychrophilum infections has only been partially elucidated, hampering the development of preventive measures to efficiently combat this disease condition. This literature review discusses the agent and the disease it causes, with emphasis on the bacterium-host interactions.

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