RESUMEN
An active immunization study using semisynthetic anti-luteinizing hormone releasing hormone (LHRH) vaccine was undertaken in adult male bonnet monkeys (Macaca radiata). Four male bonnet monkeys were immunized with LHRH-DT vaccine (100 micrograms/monkey). When the elevation of serum anti-LHRH titers reached an effective concentration, a remarkable decrease in serum testosterone level was observed. The sperm counts at 12 and 20 weeks following immunization in all immunized monkeys were reduced markedly. One representative animal with high antibody titer along with an age-matched control animal was sacrificed 20 weeks following primary immunization. The prostate weight of the immunized monkey was reduced to one-third (0.027) of the control (0.085). A reduction was also observed in other reproductive organs. Remaining animals are being studied for a reversible effect of immunization. Thus the study demonstrates that the size of the prostate can be reduced significantly by inhibition of hypothalamic-pituitary-testicular axis using this anti-LHRH vaccine.
Asunto(s)
Hormona Liberadora de Gonadotropina/inmunología , Próstata/anatomía & histología , Fosfatasa Ácida/metabolismo , Animales , Anticuerpos/análisis , Peso Corporal , Macaca radiata , Masculino , Tamaño de los Órganos , Próstata/enzimología , Próstata/patología , Recuento de Espermatozoides , Testosterona/sangre , VacunaciónRESUMEN
Histopathological changes induced by immune responses generated against luteinizing hormone releasing hormone (LHRH) were studied in adult male Wistar rats. Active immunization with a semisynthetic anti-LHRH vaccine, LHRH D-Lys6, (10, 40, and 80 micrograms/animal) conjugated with diphtheria toxoid, produced bioeffective antibodies as indicated by significant reduction in circulating testosterone levels. At the 14th week after immunization the animals were sacrificed and reproductive organs were evaluated. These organs were studied for histopathological changes and compared with those of nonimmunized control rats. Marked hypoplastic changes were observed in the genital organs. Testicular changes such as arrest of spermatogenesis at the spermatocyte level and atrophic changes in the interstitial Leydig cells were noticed in treated animals. Similarly attenuation of secretory epithelial cells with substantial increase in the stromal tissues was observed in the prostate and seminal vesicles. The current observation suggests the possible usefulness of this anti-LHRH vaccine under clinical conditions where reduction in androgenic response is desired as in the case of hormone-dependent prostatic carcinoma.
Asunto(s)
Genitales Masculinos/patología , Hormona Liberadora de Gonadotropina/inmunología , Inmunidad Activa , Vacunas Sintéticas/farmacología , Vacunas/farmacología , Animales , Apetito/efectos de los fármacos , Peso Corporal/efectos de los fármacos , Epidídimo/efectos de los fármacos , Epidídimo/patología , Epitelio/efectos de los fármacos , Epitelio/patología , Genitales Masculinos/efectos de los fármacos , Masculino , Tamaño de los Órganos/efectos de los fármacos , Próstata/efectos de los fármacos , Próstata/patología , Ratas , Ratas Endogámicas , Vesículas Seminales/efectos de los fármacos , Vesículas Seminales/patología , Testículo/efectos de los fármacos , Testículo/patología , Testosterona/sangreRESUMEN
The effect on the frequency of aflatoxin B1 (AFB1)-induced chromosomal aberrations in bone marrow cells of mice fed diets containing increasing levels of sesame oil was investigated. Mice receiving either 9% or 12% sesame oil in their diet for 8 weeks presented a statistically increased AFB1-induced aberration rate as compared to those given a diet containing 3% sesame oil. These data suggest that the metabolism of a mutagen can be influenced by the dietary lipid intake of the test animal.