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Cells ; 10(8)2021 08 10.
Artículo en Inglés | MEDLINE | ID: mdl-34440808

RESUMEN

Stratified mucin-producing intraepithelial lesion (SMILE) is a rare high-grade cervical precancerous lesion designated a variant of adenocarcinoma in situ (AIS) in the WHO classification. We aimed to determine HPV genotypes, immunohistochemical phenotype and mucin presence in SMILE. Between 2010 and 2018, SMILE was diagnosed in 34 out of 6958 (0.5%) cervical biopsies, in 23 patients. Twenty-six tissue samples from twenty-one patients were available for further analysis, including 13 with SMILE alone, 12 with SIL and/or AIS and one with HSIL, AIS and endocervical adenocarcinoma. HPV genotyping was performed using the Seegene Anyplex II HPV 28 assay. Of the 26 samples, a single HPV genotype was identified in the majority of cases (n = 22), including 12/13 SMILEs associated with SIL/AIS. All but one were high-risk HPV genotypes (23/24; 96.8%). We identified seven different HPV genotypes, the most common being HPV16 (n = 10; 43.5%), HPV18 (n = 8, 34.8%) and HPV 31 (n = 5, 21.7%). All SMILEs showed a strong positive reaction to p16, CK7, CK19 and high Ki67 expression comparable to adjacent HSIL and/or AIS if present. SMILE showed variable mucin presence and p40-positive squamous differentiation suggesting phenotypic diversity in cervical precancerous lesions infected by single HPV.


Asunto(s)
Papillomavirus Humano 16/aislamiento & purificación , Factores de Transcripción/metabolismo , Proteínas Supresoras de Tumor/metabolismo , Displasia del Cuello del Útero/patología , Neoplasias del Cuello Uterino/patología , Adenocarcinoma in Situ/patología , Adenocarcinoma in Situ/virología , Adulto , Biomarcadores de Tumor/metabolismo , Cuello del Útero/metabolismo , Cuello del Útero/patología , Inhibidor p16 de la Quinasa Dependiente de Ciclina/metabolismo , Femenino , Genotipo , Papillomavirus Humano 16/genética , Humanos , Inmunohistoquímica , Antígeno Ki-67/metabolismo , Persona de Mediana Edad , Mucinas/metabolismo , Clasificación del Tumor , Estudios Retrospectivos , Lesiones Intraepiteliales Escamosas/patología , Lesiones Intraepiteliales Escamosas/virología , Neoplasias del Cuello Uterino/metabolismo , Neoplasias del Cuello Uterino/virología , Adulto Joven , Displasia del Cuello del Útero/metabolismo , Displasia del Cuello del Útero/virología
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