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Patients undergoing radical prostatectomy for prostate cancer may experience erectile dysfunction (ED). Age of patients, experience of the surgeons and existence of ED before surgery are factors related to its appearance. The objective of the study was to assess the hemodynamic changes produced in the cavernous arteries in patients undergoing laparoscopic radical prostatectomy (LRP) measured with penile Doppler ultrasound (PDUS). A prospective database of 83 patients undergoing LRP was analysed. PDUS were performed at baseline and twelve months after surgery. International Index of Erectile Function (IIEF) and Erectile Hardness Score (EHS) questionnaires were also evaluated. A 12-month decrease in all hemodynamic parameters of both cavernous arteries was found except for the end diastolic velocity (EDV) on the left cavernous artery. Only changes between baseline and twelve-months mean values of the diameter (0.725 vs. 0.67 mm; p= 0.033) and peak systolic velocity (PSV) of the right cavernous artery (32.6 vs. 27.22 cm/s; p = 0.004) presented significant variations. The rest of the parameters were close to statistical significance, except for EDV of the right cavernous artery (p = 0.887). The erectile function domain of the IIEF showed a significant decrease (median at baseline: 26 vs. post-surgery: 7; p < 0.0001) as well as the EHS test (grade I at baseline: 2.4% vs. 12-months: 31.3%; p < 0.0001). Our study supports the idea that LRP produces local vascular injuries. A decrease in the PSV and in the diameter of both cavernous arteries was observed with PDUS and it may explain the vascular origin of ED.
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Disfunción Eréctil , Laparoscopía , Pene , Prostatectomía , Neoplasias de la Próstata , Ultrasonografía Doppler , Humanos , Masculino , Prostatectomía/métodos , Laparoscopía/métodos , Pene/irrigación sanguínea , Pene/diagnóstico por imagen , Persona de Mediana Edad , Ultrasonografía Doppler/métodos , Anciano , Disfunción Eréctil/etiología , Estudios Prospectivos , Neoplasias de la Próstata/cirugía , Neoplasias de la Próstata/diagnóstico por imagen , Hemodinámica/fisiología , Arterias/diagnóstico por imagenRESUMEN
Botrytis cinerea is the most harmful postharvest disease of table grapes. Among the strategies that can be envisaged for its control, the use of coatings based on natural products is particularly promising. The study presented herein focuses on the assessment of the antagonistic capacity of two Streptomyces species and their culture filtrates against B. cinerea. Firstly, the secondary metabolites were characterized by gas chromatography-mass spectrometry, with N1-(4-hydroxybutyl)-N3-methylguanidine acetate and 2R,3S-9-[1,3,4-trihydroxy-2-butoxymethyl]guanine acetate as the main compounds produced by S. lavendofoliae DSM 40217; and cyclo(leucyloprolyl) and cyclo(phenylalanylprolyl) as the most abundant chemical species for S. rochei DSM 41729. Subsequently, the capacity of S. lavendofoliae DSM 40217 and S. rochei DSM 41729 to inhibit the growth of the pathogen was tested in dual culture plate assays, finding 85-90% inhibition. In agar dilution tests, their culture filtrates resulted in effective concentration values (EC90) in the 246-3013 µg·mL-1 range. Upon the formation of conjugate complexes with chitosan oligomers (COS) to improve solubility and bioavailability, a synergistic behavior was observed, resulting in lower EC90 values, ranging from 201 to 953 µg·mL-1. Ex situ tests carried out on 'Timpson' and 'Red Globe' table grapes using the conjugate complexes as coatings were found to maintain the turgor of the grapes and delay the appearance of the pathogen by 10-15 days at concentrations in the 750-1000 µg·mL-1 range. Hence, the conjugate complexes of COS and the selected Streptomyces spp. culture filtrates may be put forward as promising protection treatments for the sustainable control of gray mold.
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Treatments that allow plants to better tolerate water deficit become essential, such as the application of chemical priming. In addition, it is essential to use analyses capable of measuring these effects at the biomolecular level, complementing the other physiological evaluations. In view of the above, this study aimed to evaluate the use of attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy for analyses of water deficit tolerance in Physalis peruviana plants. For this, samples of leaves, stems and roots of plants subjected to different pretreatments with proline (10 mM and 20 mM), sodium nitroprusside (SNP 25 µM and 50 µM) and H2O as control, aiming at increasing tolerance to water deficit, were evaluated. The chemical agents used attenuated water deficit in P. peruviana plants, influencing phenotypic characterization and spectral analyses. Analysis of FTIR spectra indicates that different functional groups present in leaves, stems and roots were influenced by water deficit and priming treatments. Changes in lipid levels contributed to reducing water losses by increasing the thickness of cuticular wax. Accumulation of proteins and carbohydrates promoted osmoregulation and maintenance of the water status of plants. Thus, water deficit causes changes in the functional groups present in the organs of P. peruviana, and the ATR-FTIR technique is able to detect these biomolecular changes, helping in the selection of priming treatments to increase tolerance to water deficit.
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Physalis , Agua , Análisis de Fourier , Hojas de la Planta , Plantas , Espectroscopía Infrarroja por Transformada de Fourier/métodosAsunto(s)
Yeyuno , Suturas , Anastomosis Quirúrgica/efectos adversos , Humanos , Incidencia , Yeyuno/cirugía , Suturas/efectos adversosRESUMEN
No disponible
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Humanos , Femenino , Persona de Mediana Edad , Esófago de Barrett/etiología , Esófago de Barrett/patología , Gastrectomía/efectos adversos , Obesidad Mórbida/cirugía , Cirugía Bariátrica/efectos adversos , Obesidad Mórbida/complicacionesRESUMEN
The determination of the molecular composition of plant leaves is essential to assist in nutritional management, whether for cultivated or non-cultivated species. In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments. Both species were grown under different doses of nitrogen (0, 200, 400, and 600 Kg ha-1) and leaf samples were evaluated using ATR-FTIR. Our results demonstrate that the spectra of both species were influenced by the nitrogen doses. The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield. 2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples. The P. angulata plants shows hemicellulose changes predominating followed by proteins and polysaccharides. The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them. Receiver operation characteristic analysis shows a higher value of area under the curve reflecting better reliability of the experiments carried out. Hierarchical cluster analysis shows closed separation for a similar group on dissimilarity scale. Thus the use of 2DCOS coupled with chemometrics helps to identify changes in the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization.
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Physalis , Nitrógeno , Extractos Vegetales , Hojas de la Planta , Reproducibilidad de los ResultadosRESUMEN
Due to a Production error Figs. 1 and 2 were omitted from the original article.
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No disponible
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Humanos , Historia del Siglo XVIII , Historia del Siglo XIX , Páncreas/lesiones , Neoplasias Pancreáticas/diagnóstico , Laparotomía/historia , Quiste Pancreático/diagnóstico , Procedimientos Quirúrgicos del Sistema Digestivo/historia , Laparotomía/métodos , Páncreas/patología , Yeyunostomía/historia , Neoplasias Intraductales Pancreáticas/epidemiología , Neoplasias Intraductales Pancreáticas/historia , Papiloma Intraductal/epidemiología , Papiloma Intraductal/historiaAsunto(s)
Quiste Pancreático/historia , Cistadenocarcinoma/diagnóstico , Cistadenocarcinoma/historia , Cistadenocarcinoma/cirugía , Cistoadenoma/diagnóstico , Cistoadenoma/historia , Cistoadenoma/cirugía , Drenaje , Europa (Continente) , Historia del Siglo XVIII , Historia del Siglo XIX , Historia del Siglo XX , Historia del Siglo XXI , Humanos , Laparoscopía/historia , Laparoscopía/métodos , Quiste Pancreático/clasificación , Quiste Pancreático/diagnóstico , Quiste Pancreático/cirugía , Neoplasias Intraductales Pancreáticas/diagnóstico , Neoplasias Intraductales Pancreáticas/historia , Neoplasias Intraductales Pancreáticas/cirugía , Neoplasias Pancreáticas/clasificación , Neoplasias Pancreáticas/diagnóstico , Neoplasias Pancreáticas/historia , Neoplasias Pancreáticas/cirugía , Seudoquiste Pancreático/diagnóstico , Seudoquiste Pancreático/historia , Seudoquiste Pancreático/cirugía , Pancreaticoduodenectomía/historia , Pancreatoyeyunostomía/historiaRESUMEN
No disponible
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Humanos , Femenino , Historia Antigua , Historia Medieval , Historia del Siglo XVI , Historia del Siglo XVIII , Historia del Siglo XIX , Historia del Siglo XX , Historia del Siglo XXI , Enfermedades Pancreáticas/historia , Endocrinología/historia , Gastroenterología/historia , Identidad de Género , Premio Nobel , Médicos Mujeres/historia , Nódulo de la Hermana María José/historia , Estados Unidos , Sexismo/historia , Investigadores/historiaAsunto(s)
Endocrinología/historia , Gastroenterología/historia , Identidad de Género , Enfermedades Pancreáticas/historia , Europa (Continente) , Femenino , Historia del Siglo XVI , Historia del Siglo XVIII , Historia del Siglo XIX , Historia del Siglo XX , Historia del Siglo XXI , Historia Antigua , Historia Medieval , Humanos , Premio Nobel , Médicos Mujeres/historia , Investigadores/historia , Sexismo/historia , Nódulo de la Hermana María José/historia , Estados UnidosRESUMEN
BACKGROUND: Perforation in the peritoneal cavity during transanal endoscopic microsurgery represents a major challenge. It is usually treated by primary suture, though some authors propose laparoscopic repair with or without ostomy. It is unclear whether perforation increases the risk of tumor dissemination. AIM: The purpose of the study is to assess the safety of primary suture of peritoneal perforation and the long-term risk of dissemination, also, to determine risk factors for perforation and to propose a predictive model for lesions with risk of perforation. METHOD: This is an observational study with prospective data collection at Parc Taulí University Hospital, Sabadell, of patients undergoing transanal surgery with perforation into the peritoneal cavity from June 2004 to September 2017. The main variable is postoperative morbidity and mortality. The long-term follow-up of local recurrence and peritoneal tumor dissemination is described, and a quantitative predictive model for peritoneal cavity perforation is proposed. RESULTS: Forty-five patients out of 686 (6.6%) presented perforation into the peritoneal cavity. Ten patients (22.2%) in the perforation group had morbidity, a rate similar to the non-perforated group. There was no peritoneal dissemination in patients with adenoma or with carcinoma treated with curative intent. In the quantitative predictive model, risk factors for perforation were proximal edge of tumor > 14 cm from anal verge (6 points), size ≥ 6 cm (2), age ≥ 85 years (4), anterior quadrant (3) , and sex (2). Total scores of ≥ 6 points predicted perforation. CONCLUSIONS: Primary suture after peritoneal cavity perforation during transanal surgery is safe and does not increase the risk of recurrence or peritoneal dissemination. Our predictive model provides guidance regarding the risk of perforation and the need to suture the defect after transanal surgery resection.
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Adenocarcinoma/cirugía , Adenoma/cirugía , Complicaciones Intraoperatorias/cirugía , Peritoneo/lesiones , Neoplasias del Recto/cirugía , Técnicas de Sutura , Microcirugía Endoscópica Transanal/efectos adversos , Adulto , Anciano , Anciano de 80 o más Años , Reglas de Decisión Clínica , Femenino , Estudios de Seguimiento , Humanos , Complicaciones Intraoperatorias/diagnóstico , Complicaciones Intraoperatorias/etiología , Complicaciones Intraoperatorias/prevención & control , Masculino , Persona de Mediana Edad , Peritoneo/cirugía , Pronóstico , Estudios Retrospectivos , Factores de RiesgoRESUMEN
PURPOSE: Study objectives are to determine whether quality of life is recovered completely after major injury and to identify determinants associated with a worse quality of life. METHODS: Prospective 12-month follow-up of injured patients admitted to the intensive care unit in a Spanish level 1 trauma centre. The main outcome (quality of life) was measured using the EQ-5D-5L. The relationships between sociodemographic factors, injury severity and location, and quality of life scores were evaluated. Mean comparison analysis (95% confidence interval) was performed with the student "t" test for quantitative variables and with chi-squared for proportion comparison (qualitative variables). A multivariate logistic regression (odds ratio and 95% confidence interval) was performed to identify determinants of each dimension, and a multivariate linear regression (regression coefficient and 95% confidence interval) to identify the determinants of EQus and EQvas. RESULTS: Over a 2-year period, 304 patients who met the inclusion criteria were identified, and 200 patients (65.8%) were finally included. Most of patients suffered blunt trauma (91.5%), 72.5% were men, mean age was 47.8, mean ISS was 15.2. The overall health index (EQvas) improved slightly, but its mean value at 12 months was below the Spanish population norm (P < 0.001). In the multivariate analysis, age ≥ 55, female gender and unskilled employment were risk factors for a lower EQvas. Also in the multivariate analysis, having a severe extremity injury was associated with a lower score on the mobility dimension (OR 6.56 95% CI 2.00, 21.55) while age ≥ 55 years was associated with a lower score on the usual activities dimension (OR 3.52 95% CI 1.17, 10.57). Female gender was the most important factor associated with suffering pain (OR 4.54, 95% CI 2.01, 10.27) and depression/anxiety (OR 4.04, 95% CI 1.88, 8.65). In the univariate and multivariate analyses, female gender, age ≥ 55 years, ISS ≥ 25 and severe extremity injury were associated with a lower EQ utility score (EQus). CONCLUSIONS: The quality of life score improves during the first year after major trauma. However, it does not return to the reference levels for the normal population. Female gender and age ≥ 55 years are statistically significant determinants of poorer EQvas and EQus.