RESUMEN
We characterized 3 autochthonous dengue virus serotype 3 cases and 1 imported case from 2 states in the North and South Regions of Brazil, 15 years after Brazil's last outbreak involving this serotype. We also identified a new Asian lineage recently introduced into the Americas, raising concerns about future outbreaks.
Asunto(s)
Aedes , Virus del Dengue , Dengue , Humanos , Animales , Dengue/epidemiología , Virus del Dengue/genética , Serogrupo , Brasil/epidemiología , Brotes de EnfermedadesRESUMEN
A new coronavirus [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)] is currently causing a life-threatening pandemic. In this study, we report the complete genome sequencing and genetic characterisation of a SARS-CoV-2 detected in Manaus, Amazonas, Brazil, and the protocol we designed to generate high-quality SARS-CoV-2 full genome data. The isolate was obtained from an asymptomatic carrier returning from Madrid, Spain. Nucleotide sequence analysis showed a total of nine mutations in comparison with the original human case in Wuhan, China, and support this case as belonging to the recently proposed lineage A.2. Phylogeographic analysis further confirmed the likely European origin of this case. To our knowledge, this is the first SARS-CoV-2 genome obtained from the North Brazilian Region. We believe that the information generated in this study may contribute to the ongoing efforts toward the SARS-CoV-2 emergence.
Asunto(s)
Betacoronavirus/genética , Infecciones por Coronavirus/virología , Filogenia , Neumonía Viral/virología , Infecciones Asintomáticas , Brasil , COVID-19 , Genoma Viral , Genómica , Humanos , Mutación , Pandemias , Filogeografía , SARS-CoV-2 , EspañaRESUMEN
A new coronavirus [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)] is currently causing a life-threatening pandemic. In this study, we report the complete genome sequencing and genetic characterisation of a SARS-CoV-2 detected in Manaus, Amazonas, Brazil, and the protocol we designed to generate high-quality SARS-CoV-2 full genome data. The isolate was obtained from an asymptomatic carrier returning from Madrid, Spain. Nucleotide sequence analysis showed a total of nine mutations in comparison with the original human case in Wuhan, China, and support this case as belonging to the recently proposed lineage A.2. Phylogeographic analysis further confirmed the likely European origin of this case. To our knowledge, this is the first SARS-CoV-2 genome obtained from the North Brazilian Region. We believe that the information generated in this study may contribute to the ongoing efforts toward the SARS-CoV-2 emergence.
Asunto(s)
Humanos , Filogenia , Neumonía Viral/virología , Infecciones por Coronavirus/virología , Betacoronavirus/genética , España , Brasil , Genoma Viral , Genómica , Infecciones Asintomáticas , Filogeografía , Pandemias , SARS-CoV-2 , COVID-19 , MutaciónRESUMEN
Objective: The aim of this study was to carry out an in vivo assessment of bone ingrowth in two different typesof porous titanium -the first being completely porous, and the second with a porous surface and dense nucleus,manufactured by powder metallurgy- and to evaluate their mechanical properties. Study design: Ten scaffoldsfrom each group were submitted to metallographic analysis and compression tests. Next, two scaffolds of eachtype were inserted into 14 rabbits, which were sacrificed 8 weeks after surgery. The samples were submitted forhistological examination. Results: Metallographic analysis revealed interconnected pores, and the average interconnectedpore diameter was about 360 mm, with 36% total porosity. The totally porous titanium samples and thetitanium samples with porous surface and dense nucleus showed an average compressive strength of 16.19 MPaand 69.27 MPa, respectively. After 8 weeks, the animals showed bone ingrowth, even into the most internal pores.Conclusions: The pore morphology was effective in permitting bone ingrowth in both groups. Titanium scaffoldswith a porous surface and dense nucleus showed the best mechanical properties and most adequate interface (AU)
Asunto(s)
Animales , Conejos , Oseointegración , Titanio , Fenómenos Biomecánicos , Ensayo de MaterialesRESUMEN
OBJECTIVE: The aim of this study was to carry out an in vivo assessment of bone ingrowth in two different types of porous titanium -the first being completely porous, and the second with a porous surface and dense nucleus, manufactured by powder metallurgy- and to evaluate their mechanical properties. STUDY DESIGN: Ten scaffolds from each group were submitted to metallographic analysis and compression tests. Next, two scaffolds of each type were inserted into 14 rabbits, which were sacrificed 8 weeks after surgery. The samples were submitted for histological examination. RESULTS: Metallographic analysis revealed interconnected pores, and the average interconnected pore diameter was about 360 mm, with 36% total porosity. The totally porous titanium samples and the titanium samples with porous surface and dense nucleus showed an average compressive strength of 16.19 MPa and 69.27 MPa, respectively. After 8 weeks, the animals showed bone ingrowth, even into the most internal pores. CONCLUSIONS: The pore morphology was effective in permitting bone ingrowth in both groups. Titanium scaffolds with a porous surface and dense nucleus showed the best mechanical properties and most adequate interface.