RESUMEN
p53 wild-type is a tumor suppressor gene involved in DNA gene transcription or DNA repair mechanisms. When damage to DNA is unrepairable, p53 induces programmed cell death (apoptosis). The mutant p53 gene is the most frequent molecular alteration in human cancer, including breast cancer. Here, we analyzed the genetic alterations in p53 oncogene expression in 55 patients with breast cancer at different stages and in 8 normal women. We measured by ELISA assay the serum levels of p53 mutant protein and p53 antibodies. Immunohistochemistry and RT-PCR using specific p53 primers as well as mutation detection by DNA sequencing were also evaluated in breast tumor tissue. Serological p53 antibody analysis detected 0/8 (0%), 0/4 (0%) and 9/55 (16.36%) positive cases in normal women, in patients with benign breast disease and in breast carcinoma, respectively. We found positive p53 mutant in the sera of 0/8 (0.0%) normal women, 0/4 (0%) with benign breast disease and 29/55 (52.72%) with breast carcinoma. Immunohistochemistry evaluation was positive in 29/55 (52.73%) with mammary carcinoma and 0/4 (0%) with benign breast disease. A very good correlation between p53 mutant protein detected in serum and p53 accumulation by immunohistochemistry (83.3% positive in both assays) was found in this study. These data suggest that detection of mutated p53 could be a useful serological marker for diagnostic purposes.
Asunto(s)
Biomarcadores de Tumor/genética , Neoplasias de la Mama/patología , Mutación , Proteína p53 Supresora de Tumor/genética , Adulto , Anciano , Anciano de 80 o más Años , Autoanticuerpos/sangre , Biomarcadores de Tumor/sangre , Biomarcadores de Tumor/inmunología , Neoplasias de la Mama/sangre , Neoplasias de la Mama/genética , Carcinoma in Situ/sangre , Carcinoma in Situ/genética , Carcinoma in Situ/patología , Carcinoma Ductal de Mama/sangre , Carcinoma Ductal de Mama/genética , Carcinoma Ductal de Mama/patología , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Inmunohistoquímica/métodos , Persona de Mediana Edad , Estadificación de Neoplasias , Proteína p53 Supresora de Tumor/sangre , Proteína p53 Supresora de Tumor/inmunologíaRESUMEN
A study of 191 data processing workers undertaken in Porto Alegre, Brazil, in 1989, is presented. The relationship between complaints of loss of memory and their respective scores on cognitive testing in three groups of workers, one with automatized repetitive activities and two others with diversified activities, is analysed. The scores and complaints are compared with the results of a screening test for psychiatric disorders in order to verify the influence of this confounding factor. No significant evidence of cognitive problems was found despite the high prevalence of complaints of memory loss in this population (65.4%). There is a significant statistical association between these complaints and the scores of the psychiatric testing. It is suggested that futures studies should emphasize psychiatric analysis with a view to clarifying the complaints of Data Processing workers.