RESUMEN
The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite. The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others. In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer). In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%. In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution. ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF. In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay.
Asunto(s)
Quitosano/química , Cobre/química , Durapatita/química , Povidona/química , Andamios del Tejido/química , Animales , Antiinfecciosos/química , Antiinfecciosos/farmacología , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Huesos/citología , Huesos/efectos de los fármacos , Línea Celular , Proliferación Celular/efectos de los fármacos , Quitosano/farmacología , Cobre/farmacología , Durapatita/farmacología , Humanos , Ratones , Osteoblastos/citología , Osteoblastos/efectos de los fármacos , Povidona/farmacología , Ingeniería de Tejidos/métodosRESUMEN
Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Znâ¯=â¯Mnâ¯=â¯0.05, 0.1â¯M) were prepared by hydrothermal and electrospinning methods respectively. The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology. FTIR spectra of the composite confirmed the funcitonal groups present in the composite. SEM images showed the fiber formation and the incorporation of Zn-Mn HAP into the fiber structures. The physical properties like porosity, swelling and tensile strength was studied for the prepared composites. 0.1â¯M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60â¯wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60â¯wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67â¯MPa. Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1â¯M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites. The biocompatibility of the composite was evaluated using human osteoblast cells (HOS).