RESUMEN
BACKGROUND: Volleyball is briefly described as an "interval" sport with both aerobic and anaerobic components. Exercise may influence antioxidant/prooxidant balance, which leads to differences in oxidative stress status between athletes in different sport disciplines, but the results of the previous studies are inconsistent. In this study, we aimed to determine the acute effects of exercise on oxidative stress parameters such as serum total oxidant status (TOS) and total antioxidant status (TAS) levels in volleyball players. METHODS: Thirteen male volleyball players from the same team participated in this study. The volleyball game lasted approximately 95 minutes including warm-up and cool-down periods. Blood samples were taken before the warm-up and after the cool down. Serum TOS and TAS levels were measured. Oxidative stress index (OSI), a predictor of antioxidant/prooxidant balance (TOS/TAS), was also calculated. RESULTS: The following data were revealed as median: TOS 6.84 µmol H2O2 Eq/L (95% CI: 5.80-8.13) and 5.15 (95% CI: 4.20-6.02); TAS 1.96 mmol Trolox Eq/L (95% CI: 1.91-2.08) and 1.95 (95% CI: 1.86-2.00); and OSI indexes, 3.31 (arbitrary unit) (95% CI: 2.84-4.00) and 2.64 (95% CI: 2.26-3.18) before and after the match with respectively. Serum TOS and OSI levels were significantly lower after volleyball match when compared to before (P<0.05). There was no significant difference in serum TAS levels (P>0.05). CONCLUSIONS: In individuals who exercise active sports, TOS level has been found to be decreased while TAS level has not been affected significantly after volleyball match. Our results suggested that volleyball training may not cause oxidative stress in these players. Regular physical exercise especially, volleyball training may provide adequate protection against exercise-induced oxidative stress.
Asunto(s)
Antioxidantes/metabolismo , Estrés Oxidativo , Voleibol/fisiología , Adulto , Antioxidantes/análisis , Ejercicio Físico , Femenino , Humanos , Peróxido de Hidrógeno , Masculino , Oxidantes/sangre , Especies Reactivas de Oxígeno/sangreRESUMEN
OBJECTIVES: Oxidative stress is one of the potential biochemical mechanisms involved in the pathogenesis of chronic viral hepatitis. The aim of the present study was to determine levels of oxidative stress in a large group of chronic viral hepatitis (CVH) patients who had not received antiviral treatment, and to assess the relationship between these parameters and viral load, fibrosis score and necro-inflammation of the liver. MATERIAL AND METHODS: Two hundred CVH patients and 107 healthy subjects were included in this study. Malondialdehyde, myeloperoxidase and nitrotyrosine levels were determined. RESULTS: Malondialdehyde levels were significantly higher in the CVH patients than in the control group (p < 0.05), whereas myeloperoxidase activities were significantly lower (p < 0.001). There was no statistically significant difference between nitrotyrosine levels of the patients and the controls (p > 0.05). Additionally, no significant correlation was shown between these markers and viral load, necro-inflammation and fibrosis of the liver in chronic viral hepatitis patients. CONCLUSIONS: The data from this study demonstrate that there is a disturbance in oxidative balance in patients with chronic viral hepatitis, but this imbalance was not correlated with viral load, necro-inflammatory activity or fibrosis of the liver.
Asunto(s)
Hepatitis B Crónica/sangre , Hepatitis C Crónica/sangre , Malondialdehído/sangre , Estrés Oxidativo , Peroxidasa/sangre , Tirosina/análogos & derivados , Adulto , Biomarcadores/sangre , Biopsia , Estudios de Casos y Controles , Femenino , Hepatitis B Crónica/complicaciones , Hepatitis B Crónica/diagnóstico , Hepatitis C Crónica/complicaciones , Hepatitis C Crónica/diagnóstico , Humanos , Cirrosis Hepática/diagnóstico , Cirrosis Hepática/virología , Masculino , Persona de Mediana Edad , Polonia , Tirosina/sangre , Carga Viral , Adulto JovenRESUMEN
Although atropine and oximes are traditionally used in the management of organophosphate poisoning, investigations have been directed to finding additional therapeutic approaches. Thus, the aim of this study was to evaluate the cardiac effects of magnesium sulfate pretreatment on dichlorvos intoxication in rats. Rats were randomly divided into three groups as control, dichlorvos, and magnesium sulfate groups. After 6 h of dichlorvos or corn oil (as a vehicle) injection, venous blood samples were collected, and cardiac tissue samples were obtained. Biochemical analyses were performed to measure some parameters on serum and cardiac tissue. Immunohistochemical analyses of apoptosis and inducible nitric oxide (NO) synthase showed no change in cardiac tissue. Serum cholinesterase levels were markedly depressed with dichlorvos, and further suppressed markedly with magnesium sulfate pretreatment. Although we have demonstrated that serum NO levels in dichlorvos and magnesium sulfate groups were lower than the control group, cardiac tissue NO levels in magnesium sulfate group were higher than the other two groups. Mortality was not significantly affected with magnesium sulfate pretreatment. Uncertainty still persists on the right strategies for the treatment of organophosphate acute poisoning; however, it was concluded that our results do not suggest that magnesium sulfate therapy is beneficial in the management of acute dichlorvos-induced organophosphate poisoning, and also further studies are required.