RESUMEN

We have focused attention on functions of Drosophila damaged DNA binding protein 1 (D-DDB1) in Drosophila hematopoiesis and previously reported that its whole body dsRNA over-expression using a GAL4-UAS targeted expression system results in melanotic tumors and complete lethality. Since the lesions appear to arise as a normal and heritable response to abnormal development, forming groups of cells that are recognized by the immune system and encapsulated in melanized cuticle, D-DDB1 appears to be an essential development-associated factor in Drosophila. To probe the possibility that it contributes to hemocyte development, we used a collagen promoter-GAL4 strain to over-express dsRNA of D-DDB1 in Drosophila hemocytes. The D-DDB1 gene silencing caused melanotic tumors and mortality at the end of larval development. Similarly, it interfered with melanization and synthesis of antimicrobial peptides. Transgenic flies with D-DDB1 gene silencing were found to accumulate abnormal large blood cells, reminiscent of human leukemia, suggesting that D-DDB1 has functions in hemocyte development.

Asunto(s)

Proteínas de Unión al ADN/deficiencia , Proteínas de Unión al ADN/inmunología , Proteínas de Drosophila/deficiencia , Proteínas de Drosophila/inmunología , Drosophila/inmunología , Drosophila/metabolismo , Endodesoxirribonucleasas/deficiencia , Endodesoxirribonucleasas/inmunología , Hemocitos/metabolismo , Leucemia/inmunología , Leucemia/metabolismo , Animales , Animales Modificados Genéticamente/metabolismo , Proteínas de Unión al ADN/genética , Drosophila/citología , Drosophila/genética , Proteínas de Drosophila/genética , Endodesoxirribonucleasas/genética , Hemocitos/inmunología , Hemocitos/patología , Inmunidad Innata/inmunología , Especificidad de Órganos , Proteínas Recombinantes/inmunología , Proteínas Recombinantes/metabolismo , Distribución Tisular