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The primary objective of this research was to assess microplastics (MPs) in the sediments of Chilika lake. MPs were extracted from 22 sediment samples using the density separation method combined with vacuum pump filtration. A stereo-zoom microscope and Raman spectroscopy were employed to identify the sediment-associated MPs. The total MPs collected from all 22 sites was 440 ± 3.53 particles kg-1 wet sediments, with sizes ranging between 50 and 500 µm. In terms of morphology, fibers and fragments emerged as the dominant MP types, with counts of 210 ± 1.66 and 175 ± 1.76 particles kg-1 wet sediments, respectively. Raman spectroscopy verified the presence of various MP polymers in the sediments, predominantly HDPE (37 %), followed by PS (20 %), PET (18 %), PA (11 %), PP (7 %), and PC (7 %). A notable color variation was observed in MPs; black being the most prevalent (38.8 %), succeeded by blue (19.5 %), green (11.8 %), white (11.5 %), red (10.6 %), and transparent (7.5 %). ANOVA results indicated significant (p > 0.05) variations in MP abundance across the 22 sampling locations. However, principal component analysis (PCA) and multiple regression analysis indicated that water quality parameters did not significantly influence MP abundance, yet it was found that MP retention was higher in fine-grained sediments like clay and silt. The leading sources of MPs in Chilika lake were found to be aquafarming, trailed by river and sewage discharges, fishing activities, antifouling coatings and tourism. Additionally, the pollution load index (PLI) was employed to gauge the ecological risks, categorizing the lake under risk category 1, which implies a minimal level of MPs pollution. This research aims to serve as an early warning system for MPs pollution in productive brackish water habitats globally, including Chilika lake, guiding policymakers towards appropriate management strategies and preventive measures.

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In the past few decades, extensive research and efforts have been made for developing a phase retrieval iterative algorithm (PRA) for reconstructing a complex object from far-field intensity equivalently from the object autocorrelation. Since most of the existing PRA techniques employ a random initial guess, the reconstruction output sometimes changes in different trials leading to a non-deterministic output. Additionally, the output of such algorithm occasionally either shows non-convergence, needs a longer time to converge, or shows the twin-image problem. Due to these problems, PRA methods are unsuitable for cases where consecutive reconstructed outputs need to be compared. In this Letter, a novel, to the best of our knowledge, method is developed and discussed using edge point referencing (EPR). In the EPR scheme, in addition to illuminating a region of interest (ROI) of the complex object, a small area near the periphery of the complex object within the ROI is illuminated with an additional beam. Such illumination creates an imbalance in the autocorrelation that can be used to improve the initial guess for achieving unique deterministic output free from the aforementioned problems. Furthermore, by introducing the EPR, one can also achieve faster convergence. To support our theory, derivation, simulations, and experiment are performed and presented.

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Background: Hip fractures are the most common orthopedic condition in elderly patients. In this patient group, efficient preoperative analgesia that reduces the requirement for opioids and their negative side effects is crucial. This study aims for evaluating the efficiency of fascia iliaca compartment block (FICB) and pericapsular nerve group (PENG) in providing the appropriate analgesia before positioning patients for spinal anesthesia (SA). Methods: Ninety participants undergoing hip surgeries under SA were selected randomly to receive PENG block (n = 45) using 20 mL 0.25% bupivacaine or FICB using 30 mL of 0.25% bupivacaine. The blinded observer assessed Visual Analog Scale (VAS) scores at rest, with passive leg raise to 15° before and after 30 min of the block. The blinded observer also recorded hemodynamic parameters including noninvasive blood pressure respiratory rate and heart rate. The data were analyzed using SPSS version 19. Using the appropriate statistical methodology, continuous and categorical data were analyzed, and P ≤ 0.05 was considered statistically significant. Results: At rest and with a passive leg raise to 15°, VAS scores in the PENG and FICB groups decreased significantly (P < 0.0001). After 30 minutes of performing the PENG block, the VAS scores at rest and passive leg raise were 2.16±0.67 and 3.29±0.73 respectively, whereas with the fascia iliaca block the VAS scores at rest and passive leg raise were 4.07±0.69 and 5.11±0.71 with the p = 0.001, which is highly significant. Conclusion: PENG block outperforms fascia iliaca block in providing effective analgesia before positioning patients undergoing hip surgery under SA.

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Background and Aims: In emergency and non-fasting patients posted for laparotomy under general anaesthesia, rapid sequence induction (RSI) is preferred, and it is routinely done by using succinylcholine or rocuronium. Using higher doses of atracurium [i.e. 3-4 times the 95% effective dose (ED95)] can provide acceptable intubating conditions in a short time. The primary objective of our study was to compare two different higher doses of atracurium to achieve good intubating conditions for RSI without using a priming dose. The secondary objective was to compare the duration of muscle relaxation using neuromuscular monitoring and haemodynamic responses during and after intubation. Methods: Sixty patients were enroled and randomly assigned into two groups:-, group A1 (atracurium: 0.75 mg/kg) and group A2 (atracurium: 1 mg/kg). After premedication, anaesthesia was induced with propofol 2-2.5 mg/kg and atracurium injections, followed by intubation within a minute by trained anaesthesiologists. Meanwhile, intubating conditions, neuromuscular monitoring using train-of-four (TOF) measurements and post-tetanic-count and haemodynamics were recorded. Data were analysed statistically by using the Chi-square test and Student's t-test. Results: Excellent intubation conditions (without coughing or bucking) were attained in 56.7% of cases in group A2 and in 13.3% in group A1 (P < 0.001). Duration of muscle relaxation, measured by time until TOF is two, was more prolonged in group A2 (79.2 ± 9.2 min) than in group A1 (60.13 ± 8.7 min, P < 0.001). Conclusion: Acceptable intubating conditions can be achieved in a minute with the use of a high dose of atracurium (1 mg/kg) during RSI. Hence, atracurium can be used as an alternative drug for RSI.

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A phase-retrieval algorithm can reconstruct the phase of an object from an intensity image of a diffraction pattern recorded at the Fourier plane, provided one makes a right initial guess. For the algorithms that are based on an initial random guess, at times, the output either becomes nondeterministic, becomes nonconvergent, or develops a twin image. For improving the performance of the algorithms by eliminating the twin image and for bringing uniqueness to the output, prior information about the object or more intensity measurements are needed. In order to achieve this using only a single acquisition of the intensity pattern recorded at the Fourier plane for an object, we propose a method in which object support, along with the object information in the case in which the object has a direction of asymmetry, can be measured from the object autocorrelation and utilized as an initial guess in the phase-retrieval algorithm. In the process, we explore how even a partial asymmetry in the object distribution can lead to a good solution in the phase-retrieval algorithm. This method is beneficial for unique phase detection without any twin artifact in various fields of optics. We theoretically analyze the proposed method and validate it by carrying out the simulations and experiment.

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We show a digital holographic approach for polarimetric characterization of a twisted nematic liquid crystal spatial light modulator (TNLC-SLM). An experimental scheme is designed to perform polarization analysis of the SLM with gray levels. This is realized by simultaneous detection of the polarization states of the light from the SLM for a given gray level with the help of a specially designed spatial-frequency multiplex polarization interferometer. This provides amplitude and phase characteristics of the SLM in a single shot. In order to characterize the SLM, we perform Jones matrix imaging at its various gray values (driving voltages), and corresponding results are presented. These results are expected to be useful in designing and developing various SLM-based experiments in the scalar and vectorial domain.

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The more than a century old Sagnac interferometer is put to first of its kind use to generate an achromatic single-charge vortex equivalent to a Laguerre-Gaussian beam possessing orbital angular momentum (OAM). The interference of counter-propagating polychromatic Gaussian beams of beam waist ωλ with correlated linear phase (ϕ 0 ≥ 0.025 λ) and lateral shear (y 0 ≥ 0.05 ωλ) in orthogonal directions is shown to create a vortex phase distribution around the null interference. Using a wavelength-tunable continuous-wave laser the entire range of visible wavelengths is shown to satisfy the condition for vortex generation to achieve a highly stable white-light vortex with excellent propagation integrity. The application capablitiy of the proposed scheme is demonstrated by generating ultrashort optical vortex pulses, its nonlinear frequency conversion and transforming them to vector pulses. We believe that our scheme for generating robust achromatic vortex (implemented with only mirrors and a beam-splitter) pulses in the femtosecond regime, with no conceivable spectral-temporal range and peak-power limitations, can have significant advantages for a variety of applications.

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Scattering media, such as diffused glass and biological tissue, are usually treated as obstacles in imaging. To cope with the random phase introduced by a turbid medium, most existing imaging techniques recourse to either phase compensation by optical means or phase recovery using iterative algorithms, and their applications are often limited to two-dimensional imaging. In contrast, we utilize the scattering medium as an unconventional imaging lens and exploit its lens-like properties for lensless three-dimensional (3D) imaging with diffraction-limited resolution. Our spatially incoherent lensless imaging technique is simple and capable of variable focusing with adjustable depths of focus that enables depth sensing of 3D objects that are concealed by the diffusing medium. Wide-field imaging with diffraction-limited resolution is verified experimentally by a single-shot recording of the 1951 USAF resolution test chart, and 3D imaging and depth sensing are demonstrated by shifting focus over axially separated objects.

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Discovered in 1813, the conoscopic interference pattern observed due to light propagating through a crystal, kept between crossed polarizers, shows isochromates and isogyres, respectively containing information about the dynamic and geometric phase acquired by the beam. We propose and demonstrate a closed-fringe Fourier analysis method to disentangle the isogyres from the isochromates, leading us to the azimuthally varying geometric phase and its manifestation as isogyres. This azimuthally varying geometric phase is shown to be the underlying mechanism for the spin-to-orbital angular momentum conversion observed in a diverging optical field propagating through a z-cut uniaxial crystal. We extend the formalism to study the optical activity mediated uniaxial-to-biaxial transformation due to a weak transverse electric field applied across the crystal. Closely associated with the phase and polarization singularities of the optical field, the formalism enables us to understand crystal optics in a new way, paving the way to anticipate several emerging phenomena.

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We propose a distinct concept on the generation of optical vortex through coupling between the amplitude and phase differences of the superposing beams. For the proof-of-concept demonstration, we propose a simple free-space optics recipe for the controlled synthesis of an optical beam with a vortex dipole by superposing two transversely scaled Gaussian beams. The experimental demonstration using a Sagnac interferometer introduces the desired amount of radial shear and linear phase difference between the two out-of-phase Gaussian beams to create a vortex pair of opposite topological charge in the superposed beam. Flexibility to tune their location and separation using the choice of direction of the linear phase difference and the amount of amplitude difference between the superposing beams has potential applications in optical tweezers and traps utilizing the local variation in angular momentum across the beam cross section.

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A scheme to study the effect of residual phase gradients in an optical interference between two out-of-phase Gaussian beams is proposed. In a Sagnac interferometer configured to provide a null output, a variable linear phase swept across the null point unfolds an optical field rotation due to an apparently negligible residual phase gradient present orthogonal to the linear phase sweep. As the optical beam that rotates around its propagation axis carries orbital angular momentum, the experimental results presented in this Letter could provide an insight into the momentum change associated with the energy redistribution in the fundamental phenomenon of optical interference.

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This article presents an overview of recent advances in the field of digital holography, ranging from holographic techniques designed to increase the resolution of microscopic images, holographic imaging using incoherent illumination, phase retrieval with incoherent illumination, imaging of occluded objects, and the holographic recording of depth-extended objects using a frequency-comb laser, to the design of an infrastructure for remote laboratories for digital-holographic microscopy and metrology. The paper refers to current trends in digital holography and explains them using new results that were recently achieved at the Institute for Applied Optics of the University Stuttgart.

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Retrieving the information about the object hidden around a corner or obscured by a diffused surface has a vast range of applications. Over the time many techniques have been tried to make this goal realizable. Here, we are presenting yet another approach to retrieve a 3-D object from the scattered field using digital holography with statistical averaging. The methods are simple, easy to implement and allow fast image reconstruction because they do not require phase correction, complicated image processing, scanning of the object or any kind of wave shaping. The methods inherit the merit of digital holography that the micro deformation and displacement of the hidden object can also be detected.

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Spatial and spectral information holds the key for characterizing incoherently illuminated or self-luminous objects, as well as for imaging fluorescence. We propose spectrally resolved incoherent holography using a multifunctional Mach-Zehnder interferometer that can introduce both a radial shear and a variable time delay between the interfering optical fields and permits the measurement of both spatial and temporal coherence functions, from which a 3D spatial and spectral image of the object is reconstructed. We propose and demonstrate the accurate 3D imaging of the object spectra by in situ calibration.

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The van Cittert-Zernike theorem is extended to the vectorial regime based on spatial averaging over the observation plane, and experimental demonstrations are presented. The theorem connects complex vectorial source structure to the degree of coherence and polarization of the spatially fluctuating vectorial field in the far field. Experimentation is carried out by making use of the space averages as a replacement of ensemble averages for the Gaussian stochastic field. For quantitative comparison with the theorem, analytical and experimental results are presented for a rectangular aperture with different vectorial source structures.

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The ideas of incoherent holography were conceived after the invention of coherent-light holography and their concepts seems indirectly related to it. In this work, we adopt an approach based on statistical optics to describe the process of recording of an incoherent-object hologram as a complex spatial coherence function. A Sagnac radial shearing interferometer is used for the correlation of optical fields and a Pockels cell is used to phase shift the interfering fields with the objective to quantify and to retrieve the spatial coherence function.

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We propose a new technique for achromatic 3-D field correlation that makes use of the characteristics of both axial and lateral magnifications of imaging through a common-path Sagnac shearing interferometer. With this technique, we experimentally demonstrate, for the first time to our knowledge, 3-D image reconstruction of coherence holography with generic thermal light. By virtue of the achromatic axial shearing implemented by the difference in axial magnifications in imaging, the technique enables coherence holography to reconstruct a 3-D object with an axial depth beyond the short coherence length of the thermal light.

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A simple interferometric polarimeter with an integrated calibration scheme is proposed for accurate and fast mapping of the state of polarization (SOP). Conventional single-shot polarimeters that detect the amplitude and phase of orthogonally polarized field components by interferometry using Fourier fringe analysis suffers from errors caused by the imperfect reference beam and ambiguity in the spatial carrier frequency in the fringe pattern. In the proposed system, the integrated calibration scheme eliminates those error sources and enables accurate measurement of SOP without prior knowledge of the reference beam and the spatial carrier frequency.

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We propose a novel birefringent interferometer setup for the study of unfolding points, and obtain for the first time to our knowledge the spatial polarization structure very near the unfolding point of a uniformly polarized optical vortex beam propagating in a birefringent crystal. The unfolding point is reconstructed by folding back the two separated eigen-beams at the output of the birefringent crystal into a single beam using another identical birefringent crystal, resulting in a birefringent interferometer of Mach-Zehnder type. We also demonstrate that the separation near the unfolding point can be varied by a small rotation of the second crystal.

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We report significant speckle reduction in a laser illumination system using a vibrating multimode optical fiber bundle. The optical fiber bundle was illuminated by two independent lasers simultaneously. The beams from both lasers were first expanded and collimated and were further divided into multiple beams to illuminate the fiber optic bundle with normal and oblique incidence. Static diffusers were also placed at the input and output faces of the fiber bundle, thus introducing the spatial as well as angular diversity of illumination. Experiments were carried out both in free space and in imaging geometry configuration. Standard deviation, speckle contrast and signal-to-noise ratio of the images were computed, and the results were compared with those of white light illumination. Speckle contrast close to that of white light was obtained using a vibrating fiber bundle with combined temporal, spatial, and angular diversities of the illumination.