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Genet Mol Res ; 11(4): 4198-203, 2012 Dec 06.
Artículo en Inglés | MEDLINE | ID: mdl-23315804

RESUMEN

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.


Asunto(s)
Anopheles/genética , Genes de Insecto , Insectos Vectores/genética , Repeticiones de Microsatélite , Animales , Sitios Genéticos , Genotipo , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Especificidad de la Especie
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