RESUMEN
Between October 1987 and March 1989, we tested 144 water samples obtained from the plumbing and cooling tower systems of 5 Paris hospitals for the presence of legionellae and amoebae. Of the samples tested for Legionella, 67 out of 144 (46.5%) were positive, and 82 out of 116 tested for amoebae (70.7%) were positive. The ability of protozoa to support the multiplication of legionella was shown by incubating samples at 35.5 degrees C for 7-15 days. Prior to determining the presence of legionellae and amoebae, 51 of the 144 samples were incubated. After incubation, 22 out of 25 (88%) samples which were positive for the presence of both Legionella and amoebae showed multiplication of Legionella. In 3 out of the 25 (12%) samples containing Legionella and amoebae, Legionella failed to multiply. Six out of the 51 (11.8%) samples which were negative in direct culture for Legionella but positive for amoebae, became positive after incubation. Legionella did not multiply in samples negative for amoebae, nor was there proliferation in samples after filtration through a 1.2-microns membrane followed by incubation for the same period and temperature. Strains of Legionella pneumophila serogroup 1 and serogroup 6 (SG1 and SG6), including 3 patient isolates and 2 environmental isolates, were cocultured with 2 strains of amoebae and Tetrahymena pyriformis. Plate counts, Gimenez staining and electron microscopy demonstrated that intracellular legionellae proliferation occurred.
Asunto(s)
Amoeba/aislamiento & purificación , Infección Hospitalaria/microbiología , Legionella/crecimiento & desarrollo , Microbiología del Agua , Aire Acondicionado , Amoeba/microbiología , Animales , Francia , Hartmannella/microbiología , Hartmannella/ultraestructura , Unidades Hospitalarias , Humanos , Técnicas In Vitro , Legionella/aislamiento & purificación , Microscopía Electrónica , Ingeniería Sanitaria , Tetrahymena pyriformis/microbiología , Tetrahymena pyriformis/ultraestructuraRESUMEN
The article presents data about the effect of leucopaine, a vegetable enzyme preparation, on the regenerative processes of injured tissue in rabbits as well as about the treatment of purulent wounds in 303 patients under clinical conditions. Leucopaine was shown to have positive effect on the dynamics of purulent wound repair, to facilitate wound cleaning from necrotic tissues and to promote liquidation of inflammatory reaction and purulent exudation, creating favorable conditions for active growth of granulation tissue, formation of fine, unobtrusive scar. It also shortened 1.5 times the wound repairing period.
Asunto(s)
Endopeptidasas/farmacología , Cicatrización de Heridas/efectos de los fármacos , Animales , Humanos , Extractos Vegetales/farmacología , Conejos , SupuraciónRESUMEN
A qualitative and quantitative survey of environmental Legionella in the Paris area was undertaken because of its epidemiological implications. Over a 2-year period, 322 water samples from the Paris area (unrelated to a legionnaires' disease outbreak) were examined for the presence of Legionella. No Legionella strain was isolated from the main water supplies of the municipality or from the distribution entry points of 36 buildings. Inside buildings, however, 84/190 (44.2%) tap water samples yielded one or several strains of Legionella (10(2) to 10(6) CFU/l). Contamination was significantly more frequent in hot tap water than in cold tap water. Legionella was isolated from 11 out of 14 air-conditioning systems investigated. Sixty-four of the 132 strains isolated were L. pneumophila serogroup 1. Other strains were L. pneumophila serogroups 2 to 6 (including a serogroup 4-5 variant), L. longbeachae serogroup 2 and 6 atypical Legionella.