RESUMEN
Effect of acetylsalycilic acid (aspirin; Ron-Pulenk) on activity of mouse neutrophil peroxydase system was investigated. Using luminol-dependent chemiluminscence and cytochemical methods we demonstrated that neutrophil peroxydase system in mice receiving aspirin for 14 days is probably determined by stimulation of myeloperoxydase synthesis.
Asunto(s)
Aspirina/farmacología , Neutrófilos/efectos de los fármacos , Neutrófilos/enzimología , Peroxidasa/metabolismo , Animales , Antiinflamatorios no Esteroideos/farmacología , Masculino , RatonesRESUMEN
The effect of SO3(2-), S(2-), NOs(-), and NH4(+) on activity of the peroxidase-hydrogen peroxide system in human peripheral blood neutrophils was studied by the cytochemical method. We showed that the effect of these xenobiotics on neutrophils is similar to that on plants.
Asunto(s)
Contaminantes Atmosféricos/toxicidad , Neutrófilos/efectos de los fármacos , Neutrófilos/metabolismo , Xenobióticos/toxicidad , Humanos , Peróxido de Hidrógeno/sangre , Técnicas In Vitro , Neutrófilos/inmunología , Nitratos/toxicidad , Oxidación-Reducción , Peroxidasas/sangre , Compuestos de Amonio Cuaternario/toxicidad , Sulfatos/toxicidad , Sulfuros/toxicidadRESUMEN
The effects of hypolipidemic drug clofibrate and polypeptide dalargin on activity of the neutrophil peroxidase system in mice were studied using the method of luminol-enhanced chemiluminescence. Clofibrate and dalargin increased the chemiluminescence of mouse whole blood. Their combined use several-fold potentiated this effect. It is expected that combined use of hypolipidemics and polypeptides will open a new trend in the search for stimulators of oxygen-dependent nonspecific immunity.
Asunto(s)
Antioxidantes/metabolismo , Sangre/metabolismo , Clofibrato/metabolismo , Leucina Encefalina-2-Alanina/análogos & derivados , Hipolipemiantes/metabolismo , Luminiscencia , Luminol/metabolismo , Animales , Leucina Encefalina-2-Alanina/metabolismo , Humanos , Sustancias Luminiscentes/metabolismo , Masculino , Ratones , Neutrófilos/metabolismoRESUMEN
Activity of the peroxidase-hydrogen peroxide system is proposed as a biological marker of ecological risk of heavy metal contamination of the environment. The effect of lead, copper, mercury, and cadmium ions on peroxidase system activity in human peripheral blood neutrophils was studied using cytochemical methods. A possible mechanism of suppression of peroxidase system activity by metal ions is discussed.
Asunto(s)
Monitoreo del Ambiente/métodos , Metales Pesados/toxicidad , Neutrófilos/efectos de los fármacos , Peroxidasa/antagonistas & inhibidores , Humanos , Neutrófilos/enzimología , Peroxidasa/metabolismoRESUMEN
Peroxisomes of neutrophils are formed in promyelocytes. In addition to myeloperoxidase constituting 35% peroxisomes, they contain nonenzymatic antimicrobial cationic peptides and polypeptides, several serine proteases, as well as some other hydrolases and additional components. Similar to serine proteases, these hydrolases can serve as natural antibiotics. Their function can complement the main oxidation function of neutrophilic myeloperoxidase in the protective response. The peroxisomes contain acid glycosaminoglycans functioning as an anionic carrier that reversibly binds cationic proteins, including hydrolases.
Asunto(s)
Neutrófilos/metabolismo , Peroxisomas/metabolismo , Animales , Glicosaminoglicanos/metabolismo , Humanos , Hidrolasas/metabolismo , Neutrófilos/ultraestructura , Peroxidasa/metabolismo , Serina Endopeptidasas/metabolismoRESUMEN
The effect of rare metal ions on the activity of the peroxidase system in Pisum sativum L. roots was studied by luminol-dependent chemiluminescence. Trivalent ions of scandium, gallium, indium, and lanthanum, to different extents, inhibit the chemiluminescence of damaged P. sativum roots. A decreased generation of superoxide due to the formation of the complex between metal ions and NADP can underlie the inhibited activity of peroxidase system. The possible mechanism of inhibition of the peroxidase system activity by metal ions is discussed.
Asunto(s)
Iones/farmacología , Pisum sativum/fisiología , Raíces de Plantas/fisiología , Galio/farmacología , Indicadores y Reactivos , Indio/farmacología , Lantano/farmacología , Mediciones Luminiscentes , Luminol , Pisum sativum/enzimología , Peroxidasas/metabolismo , Raíces de Plantas/enzimología , Escandio/farmacologíaRESUMEN
Some synthetic peptides increased the luminol-dependent chemiluminescence of mouse blood during phagocytosis. It is suggested that the levels of antimicrobial activity of the neutrophil peroxidase system can be raised very quickly (within some dozen of seconds) by these peptides. This raises the possibility of finding a new approach to the therapy for infectious diseases.