RESUMEN
A 1996 law concerning patients on medical procedures is still causing change in health care. While the law was intended to exempt physicians from patent infringement during the practice of medicine, its final form contains ambiquities of medical group managers should remain aware.
Asunto(s)
Medicina Clínica/legislación & jurisprudencia , Práctica de Grupo/legislación & jurisprudencia , Patentes como Asunto/legislación & jurisprudencia , Biotecnología/legislación & jurisprudencia , Humanos , Procedimientos Quirúrgicos Operativos , Estados UnidosRESUMEN
We have cloned a partial cDNA encoding murine stem cell factor (SCF) and show that the gene is syntenic with the Sl locus on mouse chromosome 10. Using retroviral vectors to immortalize fetal liver stromal cell lines from mice harboring lethal mutations at the Sl locus (Sl/Sl), we have shown that SCF genomic sequences are deleted in these lines. Furthermore, two other mutations at Sl, Sld and Sl12H, are associated with deletions or alterations of SCF genomic sequences. In vivo administration of SCF can reverse the macrocytic anemia and locally repair the mast cell deficiency of Sl/Sld mice. We have also provided biological and physical evidence that SCF is a ligand for the c-kit receptor.
Asunto(s)
Mapeo Cromosómico , Factores de Crecimiento de Célula Hematopoyética/genética , Proteínas Tirosina Quinasas/metabolismo , Proteínas Proto-Oncogénicas/metabolismo , Secuencia de Aminoácidos , Anemia Macrocítica/tratamiento farmacológico , Anemia Macrocítica/genética , Animales , Secuencia de Bases , Línea Celular , Clonación Molecular , Cricetinae , ADN/genética , ADN/aislamiento & purificación , Genes , Factores de Crecimiento de Célula Hematopoyética/metabolismo , Factores de Crecimiento de Célula Hematopoyética/uso terapéutico , Células Híbridas/citología , Ligandos , Ratones , Ratones Mutantes , Datos de Secuencia Molecular , Mutación , Proteínas Proto-Oncogénicas c-kit , Ratas , Proteínas Recombinantes/uso terapéutico , TransfecciónRESUMEN
Human or rodent bone marrow treated with recombinant human granulocyte colony-stimulating factor (rhG-CSF) in a CFU-GM assay yield predominantly granulocytic colonies. The specificity for granulocyte progenitors in vitro is also demonstrated in vivo by a five- to six-fold elevation in hamster peripheral blood neutrophils. Other cell types (monocytes, lymphocytes and eosinophils) remain stable. Analysis of mRNA from the bladder carcinoma cell line 5637 (1A6) shows the predominant species of mRNA codes for a mature protein of 174 amino acids. A small fraction of the mRNA can code for an alternative form of hG-CSF containing additional three amino acids between positions 35 and 36.
Asunto(s)
Factores Estimulantes de Colonias/genética , Granulocitos/fisiología , Animales , Línea Celular , Ensayo de Unidades Formadoras de Colonias , Cricetinae , Hematopoyesis/efectos de los fármacos , Humanos , Recuento de Leucocitos , Ratones , ARN Mensajero/genética , Proteínas Recombinantes/genética , Especificidad de la Especie , Neoplasias de la Vejiga Urinaria/genéticaRESUMEN
The myb gene is the transforming oncogene of the avian myeloblastosis virus (AMV); its normal cellular homolog, c-myb, is conserved across a broad span of evolution. In humans, c-myb is expressed in malignant hematopoietic cell lines and in primary hematopoietic tumors. Partial complementary DNA clones were generated from blast cells of patients with acute myelogenous leukemia. The sequences of the clones were compared to the c-myb of other species, as well as the v-myb of AMV. In addition, the carboxyl terminal region of human c-myb was placed in an expression vector to obtain protein for the generation of antiserum, which was used to identify the human c-myb gene product. Like v-myb, this protein was found within the nucleus of leukemic cells where it was associated with the nuclear matrix. These studies provide further evidence that c-myb might be involved in human leukemia.
Asunto(s)
Aspartato Carbamoiltransferasa , Virus de la Leucosis Aviar/genética , Virus de la Mieloblastosis Aviar/genética , Carbamoil-Fosfato Sintasa (Glutamina-Hidrolizante) , Dihidroorotasa , Leucemia Mieloide Aguda/genética , Complejos Multienzimáticos , Oncogenes , Secuencia de Bases , Línea Celular , Clonación Molecular , ADN/análisis , Enzimas de Restricción del ADN/metabolismo , Escherichia coli/genética , Células Madre Hematopoyéticas/microbiología , Humanos , Peso Molecular , Proteínas/análisisRESUMEN
Experiments were conducted to isolate and characterize the gene and gene product of a human hematopoietic colony-stimulating factor with pluripotent biological activities. This factor has the ability to induce differentiation of a murine myelomonocytic leukemia cell line WEHI-3B(D+) and cells from patients with newly diagnosed acute nonlymphocytic leukemia (ANLL). A complementary DNA copy of the gene encoding a pluripotent human granulocyte colony-stimulating factor (hG-CSF) was cloned and expressed in Escherichia coli. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay. In addition, recombinant hG-CSF can support early erythroid colonies and mixed colony formation. Competitive binding studies done with 125I-labeled hG-CSF and cell samples from two patients with newly diagnosed human leukemias as well as WEHI-3B(D+) cells showed that one of the human leukemias (ANLL, classified as M4) and the WEHI-3B(D+) cells have receptors for hG-CSF. Furthermore, the murine WEHI-3B(D+) cells and human leukemic cells classified as M2, M3, and M4 were induced by recombinant hG-CSF to undergo terminal differentiation to macrophages and granulocytes. The secreted form of the protein produced by the bladder carcinoma cell line 5637 was found to be O-glycosylated and to have a molecular weight of 19,600.
Asunto(s)
Factores Estimulantes de Colonias/farmacología , Granulocitos/fisiología , Leucemia/patología , Proteínas Recombinantes/farmacología , Animales , Diferenciación Celular/efectos de los fármacos , Línea Celular , Ensayo de Unidades Formadoras de Colonias , Factores Estimulantes de Colonias/genética , ADN/metabolismo , Escherichia coli/genética , Genes , Factor Estimulante de Colonias de Granulocitos , Humanos , Leucemia Mieloide/patología , Ratones , PlásmidosRESUMEN
The putative transforming protein of the type I human T-cell leukemia virus (HTLV-1) is a 40-kilodalton protein encoded by the X region and is termed p40XI. On the basis of both subcellular fractionation techniques and immunocytochemical analysis, it is now shown that p40XI is a nuclear protein with a relatively short half-life (120 minutes). It is synthesized de novo in considerable quantities in a human T-cell line infected with and transformed by the virus in vitro, and it is not packaged in detectable amounts in the extracellular virus.
Asunto(s)
Antígenos Virales de Tumores/metabolismo , Deltaretrovirus/metabolismo , Proteínas Virales/metabolismo , Antígenos Transformadores de Poliomavirus , Antígenos Virales de Tumores/inmunología , Fraccionamiento Celular , Línea Celular , Núcleo Celular/metabolismo , Transformación Celular Viral , Semivida , Humanos , Sueros Inmunes , Pruebas de Precipitina , Proteínas Virales/inmunologíaRESUMEN
Utilizing a survey approach, this descriptive correlational study explored the relationship between nurse involvement in the selection of a hospital information system and subsequent nurse utilization of that system. While no correlation was shown to exist between the degree of nurse involvement in the selection of a hospital information system and the subsequent nurse utilization of that system, the investigators strongly believe that further refinement of the questionnaire and investigation would show a correlation of the two.