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This study was designed to investigate the effects of Azospirillum brasilense and Bradyrhizobium sp. co-inoculation coupled with N application on soil N levels and N in plants (total N, nitrate N-NO3- and ammonium N-NH4+), photosynthetic pigments, cowpea plant biomass and grain yield. An isotopic technique was employed to evaluate 15N fertilizer recovery and derivation. Field trials involved two inoculations-(i) single Bradyrhizobium sp. and (ii) Bradyrhizobium sp. + A. brasilense co-inoculation-and four N fertilizer rates (0, 20, 40 and 80 kg ha-1). The co-inoculation of Bradyrhizobium sp. + A. brasilense increased cowpea N uptake (an increase from 10 to 14%) and grain yield (an average increase of 8%) compared to the standard inoculation with Bradyrhizobium sp. specifically derived from soil and other sources without affecting 15N fertilizer recovery. There is no need for the supplementation of N via mineral fertilizers when A. brasilense co-inoculation is performed in a cowpea crop. However, even in the case of an NPK basal fertilization, applied N rates should remain below 20 kg N ha-1 when co-inoculation with Bradyrhizobium sp. and A. brasilense is performed.

RESUMEN

With its accumulation in upland rice, cadmium (Cd) can easily enter the human food chain, which poses a global health threat considering nearly half of the human population depends on rice as a staple food source. A study was conducted to (1) evaluate Cd accumulation by rice cultivars, grown in Cd-polluted Tropical Oxisols, with different levels of Cd tolerance; (2) quantify Cd transfer from soil to rice shoots and grain; and (3) estimate daily Cd intake by humans. Three rice cultivars, characterized by low (Cateto Seda-CS), medium (BRSMG Talento-BT), and high (BRSMG Caravera-BC) Cd uptake capacity, were investigated. Rice cultivars were exposed to increasing soil Cd concentrations (0.0, 0.7, 1.3, 3.9, 7.8, and 11.7 mg kg-1). Analysis was performed on soil, shoots, and grain. Shoot biomass and grain yield decreased with increasing Cd supply, suggesting the following Cd tolerance: CS > BT > BC. Cadmium concentrations in shoots and grain increased when exposed to Cd. Only CS did not exceed the maximum Cd limit permitted in food (0.40 mg kg-1), when rates up to 1.3 mg kg-1 of Cd were applied to soil. Considering daily rice consumption levels in Brazil, Cd intake often exceeds maximum tolerable levels. Continuous monitoring of soil Cd concentrations is a pivotal step in avoiding hazards to humans. Such monitoring is important on a global scale since outside of Asia, Brazil is the leading rice-producing and rice-consuming country.

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The extraction and quantification of leaf pigments are easy, fast, and cheap procedures; on the other hand, diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy associated with chemometrics tools could offer new insights into leaf biochemical composition. We aimed to boost the classic leaf pigment quantification, adding leaf biochemical information derived from DRIFT spectroscopy + principal component analysis, using the same leaf pigment extract produced by the classical quantification method. We performed a dose-response experiment using P as the limiting nutrient, and maize (Zea mays L.) as a plant-test. After 45 d of growth, we evaluated the effects of P fertilization in total maize shoot biomass, P shoot accumulation, leaf pigment quantification by UV-Vis, and the evaluation of biochemical variations by DRIFT spectroscopy analysis associated with a chemometric approach in the same leaf extract used for pigment quantification. P fertilization raised biomass accumulation (∼7.4×), P uptake (∼2.3×), and total chlorophyll a and b contents (∼2.1×). DRIFT spectroscopy analysis of extracted pigments revealed an elevated content of proteins and polysaccharides at high P availability. At low P availability, we found a low efficiency of N metabolism suggested by the accumulation of inorganic N forms. DRIFT spectroscopy applied together with the classic leaf pigment extraction and quantification method is a novel and promising tool for plant nutrition studies as a DRIFT spectroscopy metabolic profile protocol.

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The interaction of Zn with soil compartments influences its bioavailability and uptake by plants. In this study, rice and soybean were cultivated under greenhouse conditions with the aim of evaluating Zn bioavailability and fractionation in a clayey-textured Typic Hapludox as a function of Zn rates (4 or 8 mg kg 1 Zn). The experiment was conducted until grain filling. Two soil subsamples (t 1 and t 2 ) that referred to the seeding and flowering stages, were collected and compared with two single extraction schemes, DTPA (Zn DTPAt1 and Zn DTPAt2 ) and Mehlich-1 (Zn Mt1 and Zn Mt2 ) for Zn available contents. Zn fractionation was carried out with t 2 soil subsamples for the testing of the following fractions: exchangeable Zn (Zn Exc ), Zn bound to carbonates (Zn Carb ), Zn bound to organic matter (Zn OM ), Zn bound to oxides (Zn Oxi ) and residual Zn (Zn res ). Zn applied to soil increased the Zn concentration in labile fractions in decreasing order as follows: Zn Exc > Zn OM > Zn Carb . There was no difference between the lesser or unavailable fractions, Zn Oxi and Zn res, when there was no correlation between the rates either with total accumulated Zn in plants (Zna total ), or the contents extracted by DTPA or Mehlich-1. Total cumulative Zn content in rice and soybean affected by the ZnCl 2 rates applied were positively correlated with Zn content extracted by both solutions. Both extractant solutions presented positive correlation between available contents of Zn with Zn bound to labile fractions.

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The interaction of Zn with soil compartments influences its bioavailability and uptake by plants. In this study, rice and soybean were cultivated under greenhouse conditions with the aim of evaluating Zn bioavailability and fractionation in a clayey-textured Typic Hapludox as a function of Zn rates (4 or 8 mg kg 1 Zn). The experiment was conducted until grain filling. Two soil subsamples (t 1 and t 2 ) that referred to the seeding and flowering stages, were collected and compared with two single extraction schemes, DTPA (Zn DTPAt1 and Zn DTPAt2 ) and Mehlich-1 (Zn Mt1 and Zn Mt2 ) for Zn available contents. Zn fractionation was carried out with t 2 soil subsamples for the testing of the following fractions: exchangeable Zn (Zn Exc ), Zn bound to carbonates (Zn Carb ), Zn bound to organic matter (Zn OM ), Zn bound to oxides (Zn Oxi ) and residual Zn (Zn res ). Zn applied to soil increased the Zn concentration in labile fractions in decreasing order as follows: Zn Exc > Zn OM > Zn Carb . There was no difference between the lesser or unavailable fractions, Zn Oxi and Zn res, when there was no correlation between the rates either with total accumulated Zn in plants (Zna total ), or the contents extracted by DTPA or Mehlich-1. Total cumulative Zn content in rice and soybean affected by the ZnCl 2 rates applied were positively correlated with Zn content extracted by both solutions. Both extractant solutions presented positive correlation between available contents of Zn with Zn bound to labile fractions.(AU)

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Fertilization of Eucalyptus plantations using sewage sludge on unfertile tropical soils represents an alternative to using mineral N and P fertilizers. A 44-month field experiment was conducted to study the effects of increasing application of sludge, and its interactions with mineral N and P fertilizers, on wood volume. Four rates of sludge (0, 8, 15 and 23 Mg ha-1, dry base), N (0, 47, 95 and 142 kg ha-1) and P (0, 28, 56 and 84 kg ha-1 of P2O5) were combined in a 4 × 4 × 4 factorial scheme in a totally randomized block design. Response surface and age-shift modeling was used to establish an initial recommendation for mineral fertilization of the Eucalyptus plantations treated with sludge and to analyze the implications of increased growth on the duration of the forest cycle in a tropical climate. The results showed that from 8 to 44 months after planting, the sludge application (with or without N and P) yielded a statistically larger wood volume (P < 0.05), compared to application of N and P fertilizers only. The response surface modeling showed the following outcomes: i) application of sludge based on N criterion reduced the need for N and P fertilizers by 100%; and ii) an increase in wood volume by 7% could be achieved, compared to NPK fertilizers only, if 2/3 of the recommended P was applied. The cultivation time to produce 150 m3 ha-1 of wood volume was 45 months for the control and was reduced by two, three, four, or five months, respectively, through application of recommended P, sludge dose, sludge plus one third of P, and sludge plus two thirds of P. On the whole, sewage sludge could represent an excellent unconventional N and P fertilizer source for wood production on unfertile tropical soils.

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Although silicon (Si) fertilization in rice (Oryza sativa) plants have already been studied, most of the Brazilian studies have focused on the acidity correction effects of sources and the application rate, but not on Si supply. Moreover, beneficial effects are rarely linked to other Si-accumulation plants such as pearl millet (Pennisetum glaucum), which is extensively grown in low soluble Si of Cerrado soils. The objective of this study was to evaluate the Si sources and application rates on the yield and quality of two commonly cultivated grain crops (rice and pearl millet) in Cerrado soils. The experiments were conducted on two crops (rice and pearl millet) and two soil types (Rhodic Haplustox-LV and Quartzipsamment-RQ) in a completely randomized factorial scheme with four replicates, four Si rates (0; 200; 400, and 800 kg ha-1 Si); and three sources (calcium and magnesium silicate, wollastonite, and silicic acid). All plots received the same quantities of Ca and Mg to equilibrate these levels in both soils. Ca and Mg silicate and wollastonite produced linear increases in soluble Si (0.5 mol L-1 acetic acid), in LV, RQ, and in Si uptake by rice and pearl millet. Increases in shoot dry weight were observed in rice and pearl millet from maximum rates of 542, 550 and 480 kg ha-1 Si in RQ, respectively. Ca and Mg silicate levels were higher than wollastonite in the dry weight of both plants.

Embora já tenha sido estudada a adubação silicatada em arroz, a maioria dos estudos brasileiros tem focado nos efeitos da correção da acidez de fontes e doses aplicadas e não no fornecimento de Si. Aliado a isso, os efeitos benéficos são raramente associados a outras plantas acumuladoras como o milheto, que é intensamente cultivado nos solos com baixo Si solúvel da região do Cerrado. O objetivo desse trabalho foi avaliar fontes e doses de silício na produção e qualidade de duas culturas comumente cultivadas (arroz e milheto) em solos de cerrado. Os experimentos foram conduzidos em duas culturas (arroz e milheto) e dois solos (Neossolo Quartzarênico-RQ e Latossolo Vermelho- Amarelo-LV) em um delineamento inteiramente casualizado composto de quatro doses de silício equivalentes a 0; 200; 400 e 800 kg ha-1Si, três fontes (silicato de cálcio e magnésio-S; wollastonita-W; ácido silícico-AS) e quatro repetições. Os tratamentos receberam a mesma quantidade de Ca e Mg para equilibrar as quantidades desses nutrientes dos solos. O silicato e a wollastonita aumentaram linearmente o Si solúvel em ácido acético 0,5 mol L-1 no LV e no RQ e a absorção pela parte aérea do arroz e milheto. Silicato de cálcio e magnésio e a wollastonita aumentaram linearmente o Si solúvel no solo e a absorção de Si pelo arroz e milheto. Máxima produção de grãos e massa seca da parte aérea do arroz e milheto foram obtidas com doses de 542, 550 e 480 kg ha-1 no RQ, respectivamente. As doses de Si proporcionaram redução do número de grãos chochos e aumento na absorção de Si pela parte aérea. O silicato proporcionou maior produção de massa seca comparada a wollastonita nas duas culturas.

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Large areas of land are nowadays contaminated by heavy metals and, it is therefore, important to monitor their levels in soils. Vegetables act as transfer mechanisms of such contaminants from soils to higher levels in the food chain. In this study, we aimed to evaluate the effectiveness of chemical extractants by the L-value method for Cd phytoavailability using the 109Cd radionuclide. In a greenhouse experiment, rocket plants (Eruca sativa L.) were cultivated in pots with samples from Typic Hapludox and Typic Quartzipsamment soils. Cadmium concentrations ranging from 0 to 16 mg kg-1 were added to a 200 mL solution containing 148 kBq 109Cd. The available Cd in the soil was extracted by DTPA, Mehlich-1, Mehlich-3, and a mixture of organic acids (acetic, citric, lactic, and oxalic acids). Cd concentrations were determined by atomic absorption spectrophotometry, and 109Cd radionuclide activity was measured by low-level -counting. The dry matter yield was not influenced by Cd rates, but the Cd content and accumulation in shoots had a positive linear correlation. Generally, Cd was extracted in higher quantities by Mehlich-1 followed by DTPA, Mehlich-3, and organic acids. A linear correlation was found between the chemical extractants and Cd accumulation in shoots for both soils. According to the L Ratio, the extractants based on strong acids and chelating agents presented low efficiency regarding Cd phytoavailability. The organic acids, which presented values close to the L-value, may provide a promising method for evaluating environmental contaminants.

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RESUMEN

Large areas of land are nowadays contaminated by heavy metals and, it is therefore, important to monitor their levels in soils. Vegetables act as transfer mechanisms of such contaminants from soils to higher levels in the food chain. In this study, we aimed to evaluate the effectiveness of chemical extractants by the L-value method for Cd phytoavailability using the 109Cd radionuclide. In a greenhouse experiment, rocket plants (Eruca sativa L.) were cultivated in pots with samples from Typic Hapludox and Typic Quartzipsamment soils. Cadmium concentrations ranging from 0 to 16 mg kg-1 were added to a 200 mL solution containing 148 kBq 109Cd. The available Cd in the soil was extracted by DTPA, Mehlich-1, Mehlich-3, and a mixture of organic acids (acetic, citric, lactic, and oxalic acids). Cd concentrations were determined by atomic absorption spectrophotometry, and 109Cd radionuclide activity was measured by low-level -counting. The dry matter yield was not influenced by Cd rates, but the Cd content and accumulation in shoots had a positive linear correlation. Generally, Cd was extracted in higher quantities by Mehlich-1 followed by DTPA, Mehlich-3, and organic acids. A linear correlation was found between the chemical extractants and Cd accumulation in shoots for both soils. According to the L Ratio, the extractants based on strong acids and chelating agents presented low efficiency regarding Cd phytoavailability. The organic acids, which presented values close to the L-value, may provide a promising method for evaluating environmental contaminants.(AU)

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Ingestion of vegetables containing heavy metals is one of the main ways in which these elements enter the human body. Once entered, heavy metals are deposited in bone and fat tissues, overlapping noble minerals. Slowly released into the body, heavy metals can cause an array of diseases. This study aimed to investigate the concentrations of cadmium, nickel, lead, cobalt and chromium in the most frequently consumed foodstuff in the São Paulo State, Brazil and to compare the heavy metal contents with the permissible limits established by the Brazilian legislation. A value of intake of heavy metals in human diets was also calculated to estimate the risk to human health. Vegetable samples were collected at the São Paulo General Warehousing and Centers Company, and the heavy metal content was determined by atomic absorption spectrophotometry. All sampled vegetables presented average concentrations of Cd and Ni lower than the permissible limits established by the Brazilian legislation. Pb and Cr exceeded the limits in 44 % of the analyzed samples. The Brazilian legislation does not establish a permissible limit for Co contents. Regarding the consumption habit of the population in the São Paulo State, the daily ingestion of heavy metals was below the oral dose of reference, therefore, consumption of these vegetables can be considered safe and without risk to human health.

RESUMEN

Ingestion of vegetables containing heavy metals is one of the main ways in which these elements enter the human body. Once entered, heavy metals are deposited in bone and fat tissues, overlapping noble minerals. Slowly released into the body, heavy metals can cause an array of diseases. This study aimed to investigate the concentrations of cadmium, nickel, lead, cobalt and chromium in the most frequently consumed foodstuff in the São Paulo State, Brazil and to compare the heavy metal contents with the permissible limits established by the Brazilian legislation. A value of intake of heavy metals in human diets was also calculated to estimate the risk to human health. Vegetable samples were collected at the São Paulo General Warehousing and Centers Company, and the heavy metal content was determined by atomic absorption spectrophotometry. All sampled vegetables presented average concentrations of Cd and Ni lower than the permissible limits established by the Brazilian legislation. Pb and Cr exceeded the limits in 44 % of the analyzed samples. The Brazilian legislation does not establish a permissible limit for Co contents. Regarding the consumption habit of the population in the São Paulo State, the daily ingestion of heavy metals was below the oral dose of reference, therefore, consumption of these vegetables can be considered safe and without risk to human health.

RESUMEN

Legumes as green manure are alternative sources of nitrogen (N) for crops and can supplement or even replace mineral nitrogen fertilization due to their potential for biological nitrogen fixation (BNF). The utilization of nitrogen by sugarcane (Saccharum spp.) fertilized with sunn hemp (Crotalaria juncea L.) and ammonium sulfate (AS) was evaluated using the 15N tracer technique. N was added at the rate of 196 and 70 kg ha-1 as 15Nlabeled sunn hemp green manure (SH) and as ammonium sulfate (AS), respectively. Treatments were: (i) Control; (ii) AS15N; (iii) SH15N + AS; (iv) SH15N; and (v) AS15N + SH. Sugarcane was cultivated for five years and was harvested three times. 15N recovery was evaluated in the two first harvests. In the sum of the three harvests, the highest stalk yields were obtained with a combination of green manure and inorganic N fertilizer; however, in the second cutting the yields were higher where SH was used than in plots with AS. The recovery of N by the first two consecutive harvests accounted for 19 to 21% of the N applied as leguminous green manure and 46 to 49% of the N applied as AS. The amounts of inorganic N, derived from both N sources, present in the 0-0.4 m layer of soil in the first season after N application and were below 1 kg ha-1.

Leguminosas, como adubo verde, são fontes alternativas de nitrogênio para as culturas e podem complementar ou mesmo substituir a adubação mineral nitrogenada, devido ao seu potencial de fixação biológica de nitrogênio (FBN). A utilização do nitrogênio pela cana-de-açúcar (Saccharum spp.) fertilizada com crotalária (Crotalaria juncea L.) e sulfato de amônio (SA) foi avaliada utilizando a técnica de traçador 15N. As quantidades equivalentes a 196 e 70 kg de N por hectare foram adicionados como adubo verde crotalária júncea (CJ) e como o sulfato de amônio (SA), respectivamente, nos seguintes tratamentos: (i) controle; (ii) SA15N; (iii) CJ15N + SA; (iv) CJ15N; e (v) SA15N + CJ. A cana-de-açúcar foi cultivada por cinco anos e colhida três vezes. A recuperação do 15N foi avaliada nas duas primeiras colheitas. Na soma das três safras, os maiores rendimentos de colmos foram obtidos com uma combinação de adubos verdes e fertilizantes N inorgânicos, mas, no segundo corte rendimentos superiores foram observados nos tratamentos com CJ em comparação com os observados com SA. A recuperação de N nas primeiras duas safras consecutivas representou 19 a 21% do N aplicado como adubo verde e 46 a 49% do N aplicado como SA. As quantidades de N inorgânico derivado das fontes marcadas, presentes na camada 0-0,4 m do solo na primeira safra após a aplicação do N foram inferiores a 1 kg ha-1.

RESUMEN

Legumes as green manure are alternative sources of nitrogen (N) for crops and can supplement or even replace mineral nitrogen fertilization due to their potential for biological nitrogen fixation (BNF). The utilization of nitrogen by sugarcane (Saccharum spp.) fertilized with sunn hemp (Crotalaria juncea L.) and ammonium sulfate (AS) was evaluated using the 15N tracer technique. N was added at the rate of 196 and 70 kg ha-1 as 15Nlabeled sunn hemp green manure (SH) and as ammonium sulfate (AS), respectively. Treatments were: (i) Control; (ii) AS15N; (iii) SH15N + AS; (iv) SH15N; and (v) AS15N + SH. Sugarcane was cultivated for five years and was harvested three times. 15N recovery was evaluated in the two first harvests. In the sum of the three harvests, the highest stalk yields were obtained with a combination of green manure and inorganic N fertilizer; however, in the second cutting the yields were higher where SH was used than in plots with AS. The recovery of N by the first two consecutive harvests accounted for 19 to 21% of the N applied as leguminous green manure and 46 to 49% of the N applied as AS. The amounts of inorganic N, derived from both N sources, present in the 0-0.4 m layer of soil in the first season after N application and were below 1 kg ha-1.

Leguminosas, como adubo verde, são fontes alternativas de nitrogênio para as culturas e podem complementar ou mesmo substituir a adubação mineral nitrogenada, devido ao seu potencial de fixação biológica de nitrogênio (FBN). A utilização do nitrogênio pela cana-de-açúcar (Saccharum spp.) fertilizada com crotalária (Crotalaria juncea L.) e sulfato de amônio (SA) foi avaliada utilizando a técnica de traçador 15N. As quantidades equivalentes a 196 e 70 kg de N por hectare foram adicionados como adubo verde crotalária júncea (CJ) e como o sulfato de amônio (SA), respectivamente, nos seguintes tratamentos: (i) controle; (ii) SA15N; (iii) CJ15N + SA; (iv) CJ15N; e (v) SA15N + CJ. A cana-de-açúcar foi cultivada por cinco anos e colhida três vezes. A recuperação do 15N foi avaliada nas duas primeiras colheitas. Na soma das três safras, os maiores rendimentos de colmos foram obtidos com uma combinação de adubos verdes e fertilizantes N inorgânicos, mas, no segundo corte rendimentos superiores foram observados nos tratamentos com CJ em comparação com os observados com SA. A recuperação de N nas primeiras duas safras consecutivas representou 19 a 21% do N aplicado como adubo verde e 46 a 49% do N aplicado como SA. As quantidades de N inorgânico derivado das fontes marcadas, presentes na camada 0-0,4 m do solo na primeira safra após a aplicação do N foram inferiores a 1 kg ha-1.

RESUMEN

A cana-de-açúcar (Saccharum spp.) vem sendo cultivada no Brasil para produção de açúcar e agroenergia. Em seu sistema de produção, após um ciclo de 4 a 8 anos, é possível a rotação com plantas de cobertura, antes do seu replantio, para melhoria do solo e geração de renda. Estudou-se a caracterização e produtividade de biomassa de leguminosas (como adubos-verdes) e girassol (Helianthus annuus L.), a ocorrência natural de micorrizas, o teor de açúcar e a produtividade em colmos da cana-de-açúcar IAC 87-3396 e a viabilidade econômica desse sistema com cultivo após as opções de rotação, com quantificação da produtividade durante três cortes consecutivos. O amendoim (Arachis hypogaea L.) cv. IAC-Caiapó, girassol cv. IAC-Uruguai e mucuna-preta (Mucuna aterrimum Piper and Tracy) foram as culturas que apresentaram maior percentagem de colonização por fungos micorrízicos. O girassol foi a planta de cobertura que mais extraiu nutrientes do solo, seguido por amendoim (Arachis hipogaea L.) cv. IAC-Tatu e feijão-mungo (Vigna radiata L. Wilczek). A colonização por fungos micorrízicos mostrou correlação positiva com a altura de plantas de cana no primeiro corte (p = 0,01 e R = 0,52), mas não se correlacionou com a produtividade de colmos ou açúcar. No primeiro corte, o girassol foi a cultura de rotação que ocasionou o maior aumento de produtividade, da ordem de 46% em colmos e de 50% na quantidade de açúcar, em comparação com a testemunha. Com exceção dos amendoins, todas as culturas em rotação aumentaram a renda líquida do sistema na média de três cortes de cana-de-açúcar.

Sugarcane (Saccharum spp.) is an important crop for sugar production and agro-energy purposes in Brazil. In the sugarcane production system after a 4- to 8-year cycle crop rotation may be used before replanting sugarcane to improve soil conditions and give an extra income. This study had the objective of characterizing the biomass and the natural colonization of arbuscular mycorrhizal fungi (AMF) of leguminous green manure and sunflower (Helianthus annuus L.) in rotation with sugarcane. Their effect on stalk and sugar yield of sugarcane cv. IAC 87-3396 grown subsequently was also studied. Cane yield was harvested in three subsequent cuttings. Peanut cv. IAC-Caiapó, sunflower cv. IAC-Uruguai and velvet bean (Mucuna aterrimum Piper and Tracy) were the rotational crops that resulted in the greater percentage of AMF. Sunflower was the specie that most extracted nutrients from the soil, followed by peanut cv. IAC-Tatu and mung bean (Vigna radiata L. Wilczek). The colonization with AMF had a positive correlation with sugarcane plant height, at the first cut (p = 0.01 and R = 0.52) but not with the stalk or cane yields. Sunflower was the rotational crop that brought about the greatest yield increase of the subsequent sugarcane crop: 46% increase in stalk yield and 50% in sugar yield compared with the control. Except for both peanut varieties, all rotational crops caused an increase in net income of the cropping system in the average of three sugarcane harvests.

RESUMEN

A cana-de-açúcar (Saccharum spp.) vem sendo cultivada no Brasil para produção de açúcar e agroenergia. Em seu sistema de produção, após um ciclo de 4 a 8 anos, é possível a rotação com plantas de cobertura, antes do seu replantio, para melhoria do solo e geração de renda. Estudou-se a caracterização e produtividade de biomassa de leguminosas (como adubos-verdes) e girassol (Helianthus annuus L.), a ocorrência natural de micorrizas, o teor de açúcar e a produtividade em colmos da cana-de-açúcar IAC 87-3396 e a viabilidade econômica desse sistema com cultivo após as opções de rotação, com quantificação da produtividade durante três cortes consecutivos. O amendoim (Arachis hypogaea L.) cv. IAC-Caiapó, girassol cv. IAC-Uruguai e mucuna-preta (Mucuna aterrimum Piper and Tracy) foram as culturas que apresentaram maior percentagem de colonização por fungos micorrízicos. O girassol foi a planta de cobertura que mais extraiu nutrientes do solo, seguido por amendoim (Arachis hipogaea L.) cv. IAC-Tatu e feijão-mungo (Vigna radiata L. Wilczek). A colonização por fungos micorrízicos mostrou correlação positiva com a altura de plantas de cana no primeiro corte (p = 0,01 e R = 0,52), mas não se correlacionou com a produtividade de colmos ou açúcar. No primeiro corte, o girassol foi a cultura de rotação que ocasionou o maior aumento de produtividade, da ordem de 46% em colmos e de 50% na quantidade de açúcar, em comparação com a testemunha. Com exceção dos amendoins, todas as culturas em rotação aumentaram a renda líquida do sistema na média de três cortes de cana-de-açúcar.

Sugarcane (Saccharum spp.) is an important crop for sugar production and agro-energy purposes in Brazil. In the sugarcane production system after a 4- to 8-year cycle crop rotation may be used before replanting sugarcane to improve soil conditions and give an extra income. This study had the objective of characterizing the biomass and the natural colonization of arbuscular mycorrhizal fungi (AMF) of leguminous green manure and sunflower (Helianthus annuus L.) in rotation with sugarcane. Their effect on stalk and sugar yield of sugarcane cv. IAC 87-3396 grown subsequently was also studied. Cane yield was harvested in three subsequent cuttings. Peanut cv. IAC-Caiapó, sunflower cv. IAC-Uruguai and velvet bean (Mucuna aterrimum Piper and Tracy) were the rotational crops that resulted in the greater percentage of AMF. Sunflower was the specie that most extracted nutrients from the soil, followed by peanut cv. IAC-Tatu and mung bean (Vigna radiata L. Wilczek). The colonization with AMF had a positive correlation with sugarcane plant height, at the first cut (p = 0.01 and R = 0.52) but not with the stalk or cane yields. Sunflower was the rotational crop that brought about the greatest yield increase of the subsequent sugarcane crop: 46% increase in stalk yield and 50% in sugar yield compared with the control. Except for both peanut varieties, all rotational crops caused an increase in net income of the cropping system in the average of three sugarcane harvests.

RESUMEN

En el cultivo de papa en invernadero, la nutrición mineral es uno de los factores que más contribuye para alcanzar elevado rendimiento y calidad del producto. El conocimiento de la cantidad de nutrientes acumulada en la planta en cada fase de crecimiento suministra información que ayuda en el establecimiento de fertilizaciones más balanceadas. El objetivo de este trabajo fue determinar las curvas de absorción y acumulación de macronutrientes en plantas de papa para la producción de tubérculos-semilla de papa en solución nutritiva. El experimento fue ejecutado en condiciones de invernadero, utilizando material in vitro de categoría pre-básica de la variedad ‘Atlantic’. El inicio de las colectas fue a partir de los 14 días después del transplante (DDT), con frecuencia semanal y se prolongó hasta los 70 DDT. El diseño experimental empleado fue el de bloques completos al azar con nueve tratamientos referentes a las épocas de colectas de las plantas y cuatro repeticiones. La mayor demanda de nutrientes en la parte aérea ocurrió en el periodo comprendido entre los 28 y 56 DDT, mientras que en los tubérculos fue a partir de los 49 DDT. La secuencia de acumulación máxima de los macronutrientes fue K>N>S>Ca>P>Mg.

In greenhouse potato cultivation, mineral nutrition is one of the main factors contributing to high yields and better product quality. Knowledge about the amount of nutrients accumulated in the plants at each growing phase provides important information that helps the establishment of a more balanced fertilizer application. The objective of this research was to determine the time course of macronutrients uptake and accumulation in potato plants for seed-tuber production, grown in nutrient solution. The experiment was carried out in a greenhouse, using in vitro material from the pre-basic category of the ‘Atlantic’ variety. The plants were collected weekly from 14 days after transplanting (DAT) until 70 DAT. The experimental design was a completely randomized block with 9 treatments to sampling times and four replicates. The highest nutrient requirement in the plant shoot occurred at the periods between 28 and 56 DAT, while in the tubers it was after 49 DAT. The maximum accumulation sequence of macronutrients was K>N>S>Ca>P>Mg.

No cultivo da batateira em casa-de-vegetação, a nutrição mineral é um dos fatores que mais contribui para atingir elevada produtividade e qualidade do produto. O conhecimento da quantidade de nutrientes acumulada na planta em cada fase de crescimento fornece informação importante que ajuda no estabelecimento de adubações mais balanceadas. O objetivo de este trabalho foi determinar as curvas de absorção e acúmulo de macronutrientes em plantas de batata para a produção de tubérculos-semente de batata, em solução nutritiva. O experimento foi executado em condições de casa-de-vegetação, utilizando material in vitro, da categoria pre-básica do cultivar ‘Atlantic’. O inicio das coletas foi a partir dos 14 dias após do transplante (DAT), com freqüência semanal y se prolongou até os 70 DAT. O delineamento experimental empregado foi o de blocos completos ao acaso, com quatro repetições e nove tratamentos, referentes às épocas de coletas das plantas. A maior demanda de nutrientes na parte aérea ocorreu no período compreendido entre 28 e 56 DAT, enquanto nos tubérculos foi a partir dos 49 DAT. A seqüência de acúmulo máximo dos macronutrientes foi K>N>S>Ca>P>Mg.

RESUMEN

The availability of phosphorus (P) from " Patos de Minas" phosphate rock (PR) can be improved if it is applied mixed with a water-soluble P source. The objective of this study was to evaluate 32P as a tracer to quantify the effect of the ratio of mixtures of triple superphosphate (TSP) with PR and the rates of application on P availability from PR. Two experiments were conducted in a greenhouse utilizing corn (Zea mays L.) plants as test crop. In the first experiment, the P sources were applied at the rate of 90 mg P kg-1 soil either separately or as compacted mixtures in several TSP:PR ratios (100:0, 80:20, 60:40, 50:50, 40:60, 20:80 and 0:100 calculated on the basis of the total P content). In the second experiment, the TSP was applied alone or as 50:50 compacted mixtures with PR applied at four P rates (15, 30, 60 and 90 mg P kg-1) while the sole PR treatment was applied at the 90 mg kg-1 P rate . The mixture of PR with TSP improved the P recovery from PR in the corn plant and this effect increased proportionally to the TSP amounts in the mixture. When compared with the plant P recovery from TSP (10.52%), PR-P recovery (2.57%) was much lower even when mixed together in the ratio of 80% TSP: 20% PR. There was no difference in PR-P utilization by the corn plants with increasing P rates in the mixture (1:1 proportion). Therefore, PR-P availability is affected by the proportions of the mixtures with water soluble P, but not by P rates.

A disponibilidade de fósforo do fosfato natural de Patos de Minas (FN) pode ser melhorada se aplicado junto com uma fonte de P solúvel em água. O objetivo desse estudo foi usar o 32P como traçador para quantificar o efeito das doses e das proporções das misturas de superfosfato triplo (SFT) com FN no aumento da disponibilidade de P do FN. Dois experimentos foram desenvolvidos em casa-de-vegetação com plantas de milho (Zea mays L.) como cultura teste. No primeiro experimento as fontes de fósforo, na dose de 90 mg kg-1 de P, foram aplicadas sozinhas ou em misturas compactadas e em várias proporções de SFT com FN (80:20, 60:40, 50:50, 40:60 e 20:80) calculadas com base no teor de P total, enquanto que no segundo, o superfosfato triplo foi aplicado tanto sozinho como em misturas compactadas com o fosfato natural de Patos e em quatro doses de P (15, 30, 60 e 90 mg kg-1) na proporção de 50:50 e o FN sozinho na dose de 90 mg P kg-1. A mistura do FN com o SFT melhorou o aproveitamento do P do FN pelo milho e esse efeito foi crescente com o aumento da proporção do SFT na mistura. Se comparado com o aproveitamento do P do SFT (10,52%) pelas plantas o aproveitamento do P do FN (2,57%) foi baixo, mesmo na proporção de 80% SFT: 20% FN. Não houve diferença no aproveitamento do P do PR entre as doses da mistura na proporção de 1:1. Portanto, a disponibilidade de P do FN é afetada pela proporção das misturas com a fonte solúvel de P, mas não pelas doses deste nutriente.

RESUMEN

Due to their nitrogen fixation potential, legumes represent an alternative for supplying nutrients, substituting or complementing mineral fertilization in cropping systems involving green manuring. The objective of this study was to evaluate the N balance in a soil-plant system involving green manures [sunn hemp (Crotalaria juncea L.) and velvet bean (Mucuna aterrima Piper & Tracy)], both labeled with 15N. They were incorporated into two soils of contrasting textural classes: a clayey Eutrudox and a sandy-clayey Paleudalf, both cultivated with corn. The research was carried out in a greenhouse, using pots containing 6 kg of air dried soil, to which the equivalent to 13 Mg ha-1 dry matter of above-ground mass plus 2.7 or 2.2 Mg ha-1 of velvet bean and sunn hemp roots were incorporated, respectively, with 15N labeling of either shoots or roots. One hundred days after emergence of the corn, the velvet bean residues provided higher accumulation of N in the soil, higher absorption by corn plants and accumulation in the shoot. The green manure decomposition was more intense in the medium-textured Paleudalf. The highest nitrogen losses were also observed in this soil.

Em função de seu potencial de fixação de nitrogênio, as leguminosas representam uma alternativa ao suprimento, substituição ou complementação da adubação mineral em sistemas de cultivos envolvendo adubação verde. O objetivo deste estudo foi avaliar o balanço do N no sistema solo planta com adubos verdes crotalária júncea (Crotalaria juncea L.) e mucuna-preta (Mucuna aterrima Piper & Tracy), marcadas com 15N, incorporadas em dois solos de diferentes classes texturais: Latossolo Vermelho eutroférrico textura argilosa, A moderado (LVef) e Argissolo Vermelho-Amarelo distrófico textura arenosa/média, A moderado (PVAd), e cultivados com milho. O trabalho foi desenvolvido em casa-de-vegetação, em vasos contendo 6 kg de terra aos quais foi incorporado o equivalente a 13 Mg ha-1 de massa seca da parte aérea e 2,7 ou 2,2 Mg ha-1 de raízes de mucuna-preta e de crotalária júncea, respectivamente. A marcação com 15N foi efetuada ou nas raízes ou na parte aérea. Cem dias após emergência do milho, a incorporação de mucuna-preta aos solos proporcionou maior acúmulo de nitrogênio no solo, maior absorção do elemento pelas plantas de milho e sua acumulação na parte aérea. A decomposição da parte aérea e raízes dos adubos verdes foi mais intensa no solo de textura média (PVAd). Neste solo, também, foram observadas as maiores perdas de nitrogênio.

RESUMEN

Due to their nitrogen fixation potential, legumes represent an alternative for supplying nutrients, substituting or complementing mineral fertilization in cropping systems involving green manuring. The objective of this study was to evaluate the N balance in a soil-plant system involving green manures [sunn hemp (Crotalaria juncea L.) and velvet bean (Mucuna aterrima Piper & Tracy)], both labeled with 15N. They were incorporated into two soils of contrasting textural classes: a clayey Eutrudox and a sandy-clayey Paleudalf, both cultivated with corn. The research was carried out in a greenhouse, using pots containing 6 kg of air dried soil, to which the equivalent to 13 Mg ha-1 dry matter of above-ground mass plus 2.7 or 2.2 Mg ha-1 of velvet bean and sunn hemp roots were incorporated, respectively, with 15N labeling of either shoots or roots. One hundred days after emergence of the corn, the velvet bean residues provided higher accumulation of N in the soil, higher absorption by corn plants and accumulation in the shoot. The green manure decomposition was more intense in the medium-textured Paleudalf. The highest nitrogen losses were also observed in this soil.

Em função de seu potencial de fixação de nitrogênio, as leguminosas representam uma alternativa ao suprimento, substituição ou complementação da adubação mineral em sistemas de cultivos envolvendo adubação verde. O objetivo deste estudo foi avaliar o balanço do N no sistema solo planta com adubos verdes crotalária júncea (Crotalaria juncea L.) e mucuna-preta (Mucuna aterrima Piper & Tracy), marcadas com 15N, incorporadas em dois solos de diferentes classes texturais: Latossolo Vermelho eutroférrico textura argilosa, A moderado (LVef) e Argissolo Vermelho-Amarelo distrófico textura arenosa/média, A moderado (PVAd), e cultivados com milho. O trabalho foi desenvolvido em casa-de-vegetação, em vasos contendo 6 kg de terra aos quais foi incorporado o equivalente a 13 Mg ha-1 de massa seca da parte aérea e 2,7 ou 2,2 Mg ha-1 de raízes de mucuna-preta e de crotalária júncea, respectivamente. A marcação com 15N foi efetuada ou nas raízes ou na parte aérea. Cem dias após emergência do milho, a incorporação de mucuna-preta aos solos proporcionou maior acúmulo de nitrogênio no solo, maior absorção do elemento pelas plantas de milho e sua acumulação na parte aérea. A decomposição da parte aérea e raízes dos adubos verdes foi mais intensa no solo de textura média (PVAd). Neste solo, também, foram observadas as maiores perdas de nitrogênio.

RESUMEN

The availability of phosphorus (P) from " Patos de Minas" phosphate rock (PR) can be improved if it is applied mixed with a water-soluble P source. The objective of this study was to evaluate 32P as a tracer to quantify the effect of the ratio of mixtures of triple superphosphate (TSP) with PR and the rates of application on P availability from PR. Two experiments were conducted in a greenhouse utilizing corn (Zea mays L.) plants as test crop. In the first experiment, the P sources were applied at the rate of 90 mg P kg-1 soil either separately or as compacted mixtures in several TSP:PR ratios (100:0, 80:20, 60:40, 50:50, 40:60, 20:80 and 0:100 calculated on the basis of the total P content). In the second experiment, the TSP was applied alone or as 50:50 compacted mixtures with PR applied at four P rates (15, 30, 60 and 90 mg P kg-1) while the sole PR treatment was applied at the 90 mg kg-1 P rate . The mixture of PR with TSP improved the P recovery from PR in the corn plant and this effect increased proportionally to the TSP amounts in the mixture. When compared with the plant P recovery from TSP (10.52%), PR-P recovery (2.57%) was much lower even when mixed together in the ratio of 80% TSP: 20% PR. There was no difference in PR-P utilization by the corn plants with increasing P rates in the mixture (1:1 proportion). Therefore, PR-P availability is affected by the proportions of the mixtures with water soluble P, but not by P rates.

A disponibilidade de fósforo do fosfato natural de Patos de Minas (FN) pode ser melhorada se aplicado junto com uma fonte de P solúvel em água. O objetivo desse estudo foi usar o 32P como traçador para quantificar o efeito das doses e das proporções das misturas de superfosfato triplo (SFT) com FN no aumento da disponibilidade de P do FN. Dois experimentos foram desenvolvidos em casa-de-vegetação com plantas de milho (Zea mays L.) como cultura teste. No primeiro experimento as fontes de fósforo, na dose de 90 mg kg-1 de P, foram aplicadas sozinhas ou em misturas compactadas e em várias proporções de SFT com FN (80:20, 60:40, 50:50, 40:60 e 20:80) calculadas com base no teor de P total, enquanto que no segundo, o superfosfato triplo foi aplicado tanto sozinho como em misturas compactadas com o fosfato natural de Patos e em quatro doses de P (15, 30, 60 e 90 mg kg-1) na proporção de 50:50 e o FN sozinho na dose de 90 mg P kg-1. A mistura do FN com o SFT melhorou o aproveitamento do P do FN pelo milho e esse efeito foi crescente com o aumento da proporção do SFT na mistura. Se comparado com o aproveitamento do P do SFT (10,52%) pelas plantas o aproveitamento do P do FN (2,57%) foi baixo, mesmo na proporção de 80% SFT: 20% FN. Não houve diferença no aproveitamento do P do PR entre as doses da mistura na proporção de 1:1. Portanto, a disponibilidade de P do FN é afetada pela proporção das misturas com a fonte solúvel de P, mas não pelas doses deste nutriente.