RESUMEN
In this study, the biosurfactants (Bio-SFs) producing bacteria are screened from the selected alkaline lake of Ethiopia, and the potential bacterial strain and their produced Bio-SFs are further characterized. In an initial screening, 25 bacterial isolates were isolated, and among those, the bacterial isolate assigned as CS1 was identified as the most potent producer of Bio-SFs using a subsequent characterization process. The CS1 strain was identified as Serratia sp. via biochemical and molecular methods. An emulsion index (E24) of 69.06 ± 0.11% was obtained for CS1 after 5 days of incubation time at 30 °C. The CS1-extracted Bio-SFs were characterized by Fourier transform infrared (FTIR), and it indicated that the type of biosurfactant produced was a glycolipid. The stability of the crude Bio-SFs was characterized, and the optimal conditions were found to be 80 °C, pH 8, and 3% NaCl, respectively. The extracted Bio-SFs were compatible with tested commercial detergents, and its efficiency increased from 12.2 ± 0.1% to 67.1 ± 0.17% and 70.43 ± 0.11% when combined with commercially available detergent brands in Ethiopia such as Taza and Largo, respectively. This study suggests that the isolated S. marcescens CS1 strain has the potential to produce Bio-SFs that are viable competence to replace the use of synthetic chemicals in the production of commercial detergents.
Asunto(s)
Detergentes , Tensoactivos , Emulsiones , Monitoreo del Ambiente , Etiopía , Glucolípidos , Lagos , Serratia , Cloruro de Sodio , Tensoactivos/químicaRESUMEN
Objective: To compare the phytochemical and antimicrobial activity of Ethiopian Kale leaves infusions, investigate the antioxidant activity and profile the major phytochemicals existing in the better solvent system. Methods: Ethiopian Kale leaves were collected from Addis Ababa, Ethiopia, and extracted using different solvents. The qualitative phytochemical analysis, antibacterial assays, and Fourier Transform Infrared (FTIR) analysis are executed for all extracts. Antioxidant assay and Gas Chromatography-Mass Spectrometry (GC-MS) analysis are carried out for the solvent system, which showed better activity in preliminary studies. Results: The qualitative phytochemical analysis exposed the presence of different classes of phytoconstituents in most of the tested extracts. The broad spectrum of antibacterial activity (7-15 mm) was noted against the tested bacterial species. The functional groups of the extracts are reported by FTIR analysis. The antioxidant ability of ethanol extract was found to be (62.92 ± 0.34)% for 2,2-diphenyl-1-picrylhydrazyl (DPPH*) assay and (71.12 ± 0.41)% for 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. More than 17 major phytocompounds in ethanol extract were profiled by GC-MS analysis. Conclusion: The ethanol extract of Ethiopian Kale leaves contain a good source of phytochemicals and it can be a significant source for various functional applications.