RESUMEN
Guanosine triphosphate (GTP)-binding proteins are involved in controlling a wide range of fundamental cellular processes. In vitro studies have indicated a role for GTP during Drosophila P element transposition. Here we show that P element transposase contains a non-canonical GTP-binding domain that is critical for its ability to mediate transposition in Drosophila cells. Moreover, a single amino acid substitution could switch the nucleotide binding-specificity of transposase from GTP to xanthosine triphosphate (XTP). Importantly, this mutant protein could no longer function effectively in transposition in vivo but required addition of exogenous xanthine or xanthosine for reactivation. These results suggest that transposition may be controlled by physiological GTP levels and demonstrate that a single mutation can switch the nucleotide specificity for a complex cellular process in vivo.
Asunto(s)
ADN Nucleotidiltransferasas/metabolismo , Elementos Transponibles de ADN , Drosophila/enzimología , Guanosina Trifosfato/metabolismo , Ribonucleótidos/metabolismo , Animales , Línea Celular , Secuencia de Consenso , ADN Nucleotidiltransferasas/química , ADN Nucleotidiltransferasas/genética , Drosophila/genética , Electroforesis en Gel de Poliacrilamida , Immunoblotting , Mutación , Plásmidos/genética , Unión Proteica , Ribonucleósidos/farmacología , Transposasas , Xantina , Xantinas/farmacologíaRESUMEN
Drosophila P elements are mobile DNA elements that encode an 87-kDa transposase enzyme and transpositional repressor proteins. One of these repressor proteins is the 207-amino-acid KP protein which is encoded by a naturally occurring P element with an internal deletion. To study the molecular mechanisms by which KP represses transposition, the protein was expressed, purified, and characterized. We show that the KP protein binds to multiple sites on the ends of P-element DNA, unlike the full-length transposase protein. These sites include the high-affinity transposase binding site, an 11-bp transpositional enhancer, and, at the highest concentrations tested, the terminal 31-hp inverted repeats. The DNA binding domain was localized to the N-terminal 98 amino acids and contains a CCHC sequence, a potential metal binding motif. We also demonstrate that the KP repressor protein can dimerize and contains two protein-protein interaction regions and that this dimerization is essential for high-affinity DNA binding.
Asunto(s)
Elementos Transponibles de ADN , ADN/metabolismo , Drosophila melanogaster/fisiología , Proteínas Represoras/metabolismo , Secuencia de Aminoácidos , Animales , Sitios de Unión , ADN/aislamiento & purificación , ADN Nucleotidiltransferasas/biosíntesis , ADN Nucleotidiltransferasas/genética , Sondas de ADN , Dimerización , Leucina Zippers , Metales/metabolismo , Mutagénesis Sitio-Dirigida , Reacción en Cadena de la Polimerasa , Proteínas Represoras/biosíntesis , Proteínas Represoras/química , Proteínas Represoras/genética , Proteínas Represoras/aislamiento & purificación , TransposasasRESUMEN
Precursor RNA transcribed from the yeast mitochondrial gene coding for the large ribosomal RNA contains a group I intron that can excise itself in vitro. Apart from group I specific sequence elements the intron also contains a gene encoding a DNA endonuclease involved in intron dispersal. A precursor RNA derivative from which this gene has been removed self-splices efficiently, but due to activation of cryptic opening sites located in the 5' exon, the 3' part of this exon is sometimes co-excised with the intron. Upon further reaction, this enlarged intron molecules give rise to interlocked circles, comprising small circles derived from 5' exon parts and large circles of the intron. Sequence comparison between cryptic opening sites and authentic splice sites reveals in most cases homology with the 3' exon part that is capable of interacting with the Internal Guide Sequence. The role of the IGS was further substantiated by replacing the cryptic opening sites with well defined sequences of authentic splice sites: one corresponding to the 3' splice site and its mutant derivatives, the other to a fragment containing the natural 5'-3' exon junction. Precursor RNAs derived from these constructs give rise to interlocked circles, and mutation studies confirm that the 3' exon nucleotides flanking a 3' splice site are essential for their formation. The results underline the crucial role of the IGS in interlocked circle formation which behaves similarly as in the normal self-splicing reactions. It has been proposed that the two short helices formed by basepairing of the IGS with the 5' and 3' exon can co-axially stack on top of each other forming a quasi continuous RNA double helix or pseudoknot. We present a model explaining how transesterification reactions of a mutant precursor RNA in such a pseudoknot can lead to interlocked circles. The experiments support the notion that a similar structure is also operative in splicing of wild type precursor RNA.
Asunto(s)
Intrones , Precursores del ARN/metabolismo , Secuencia de Bases , Clonación Molecular , ADN , Electroforesis en Gel Bidimensional , Esterificación , Datos de Secuencia Molecular , Precursores del ARN/química , Empalme del ARN , Relación Estructura-ActividadRESUMEN
Initiation of adenovirus DNA replication in vitro minimally requires the viral TP-DNA template and the precursor terminal protein-DNA polymerase heterodimer (pTP-pol). Optimal initiation occurs in the presence of the cellular transcription factors NFI and Oct-1 and the viral DNA binding protein (DBP). We have studied the influence of these three stimulatory proteins on the kinetics of formation of the pTP-dCMP initiation complex. NFI increases the Vmax of the reaction but does not affect the apparent Km for dC-TP. This indicates that NFI acts by enlarging the amount of active initiation complex in agreement with its stabilizing effect on binding of pTP-pol to the template. Similar kinetic effects were observed for Oct-1. Since Oct-1 does not stabilize binding of pTP-pol to the origin this suggests that Oct-1 increases the rate of pTP-dCMP formation. DBP stimulates the initiation reaction in two ways. First, it moderately increases the Vmax at suboptimal NFI concentrations, which is related to its enhancing effect on binding of NFI to the origin. Second, a much larger stimulation was caused by DBP itself based on a reduction of the Km for dCTP, which was independent of the concentration of pTP-pol or NFI. The Km for dCTP during initiation is lower than during elongation.
Asunto(s)
Adenoviridae/genética , Proteínas Potenciadoras de Unión a CCAAT , Replicación del ADN , ADN Viral/metabolismo , Proteínas de Unión al ADN/metabolismo , Factores de Transcripción/metabolismo , Adenoviridae/metabolismo , Secuencia de Aminoácidos , Citidina Trifosfato/metabolismo , ADN Viral/biosíntesis , Factor C1 de la Célula Huésped , Cinética , Datos de Secuencia Molecular , Factores de Transcripción NFI , Factor 1 de Transcripción de Unión a OctámerosRESUMEN
POU domain proteins constitute a family of eukaryotic transcription factors that exert critical functions during development. They contain a conserved 160 amino acids DNA binding domain, the POU domain. Genetic data have demonstrated that some POU domain proteins are essential for the proliferation of specific cell types, suggesting a possible role in DNA replication. In addition, the ubiquitous POU transcription factor Oct-1 or its isolated POU domain enhances adenovirus DNA replication. Here we compared the DNA binding specificities of POU domain proteins from different subclasses. They exhibit overlapping, yet distinct binding site preferences. Furthermore, purified Pit-1, Oct-1, Oct-2, Oct-6, Oct-4 and zebrafish POU[C] could all stimulate adenovirus DNA replication in a reconstituted in vitro system. Thus, activation appears to depend on a property common to most POU domain proteins. Adenovirus DNA replication is also stimulated by the transcription factor NFI/CTF. In contrast to NFI, the POU domain did not enhance binding of precursor terminal protein-DNA polymerase to the origin nor did it stabilize the preinitiation complex. These results suggest that the POU domain acts on a rate limiting step after formation of the preinitiation complex.
Asunto(s)
Adenoviridae/genética , Replicación del ADN , Proteínas de Unión al ADN/metabolismo , Factores de Transcripción/metabolismo , Secuencia de Bases , Sitios de Unión , ADN Viral/biosíntesis , ADN Viral/metabolismo , Proteínas de Unión al ADN/clasificación , Datos de Secuencia Molecular , Factores del Dominio POU , Factores de Transcripción/clasificaciónRESUMEN
Nuclear factor I (NFI) or its isolated DNA-binding domain (NFI-BD) enhances initiation of adenovirus DNA replication up to 50-fold at low concentrations of the precursor terminal protein-DNA polymerase (pTP-pol) complex. Both in solution and when bound to DNA, NFI-BD can form a complex with pTP-pol. To investigate the mechanism of enhancement by NFI, we determined the stability of a functional preinitiation complex formed in vitro between pTP-pol and the origin. Challenge experiments with a distinguishable template containing an identical origin revealed that in the absence of NFI, this preinitiation complex was very sensitive to competition for pTP-pol. Addition of NFI-BD increased the half-life of the complex at least 10-fold and led to the formation of a template-committed preinitiation complex. In agreement with this, binding of pTP-pol to origin DNA in band-shift assays was enhanced by NFI. By DNase I footprinting we show that the specificity of binding as well as induction of structural changes in origin DNA by pTP-pol are increased by NFI. These results indicate that NFI, by binding and positioning pTP-pol, stabilizes the complex between pTP-pol and the core origin, and thus enhances initiation of DNA replication.
Asunto(s)
Adenovirus Humanos/genética , Proteínas Potenciadoras de Unión a CCAAT , Replicación del ADN , ADN Viral/genética , Proteínas de Unión al ADN/metabolismo , Factores de Transcripción/metabolismo , Proteínas de Unión al ADN/genética , ADN Polimerasa Dirigida por ADN/genética , ADN Polimerasa Dirigida por ADN/metabolismo , Desoxirribonucleasa I , Productos del Gen pol/genética , Productos del Gen pol/metabolismo , Células HeLa , Humanos , Immunoblotting , Cinética , Modelos Genéticos , Factores de Transcripción NFI , Proteínas Nucleares , Moldes Genéticos , Virus Vaccinia/genética , Proteína 1 de Unión a la Caja YRESUMEN
Initiation of adenovirus DNA replication is strongly enhanced by two transcription factors, nuclear factor I (NFI) and nuclear factor III (NFIII/oct-1). These proteins bind to two closely spaced recognition sequences in the origin. We produced NFI and NFIII/oct-1, as well as their biologically active, replication-competent DNA-binding domains (NFI-BD and the POU domain), in a vaccinia virus expression system and purified these polypeptides to apparent homogeneity. By DNase I footprinting and gel retardation, we show that the two proteins, as well as their purified DNA-binding domains, bind independently and without cooperative effects to their recognition sequences. By using a reconstituted system consisting of the purified viral proteins (precursor terminal protein-DNA polymerase complex (pTP-pol) and DNA-binding protein, we show that NFIII/oct-1 or the POU domain stimulates DNA replication in the absence of NFI or NFI-BD and vice versa. When added together, the enhancing effect of the two transcription factors was independent and nonsynergistic. Interestingly, stimulation by NFI or NFI-BD was strongly dependent on the concentration of the pTP-pol complex. At low pTP-pol concentrations, NFI or NFI-BD stimulated up to 50-fold, while at high concentrations, the stimulation was less than twofold, indicating that the need for NFI can be overcome by high pTP-pol concentrations. In contrast, stimulation by NFIII/oct-1 or the POU domain was much less dependent on the pTP-pol concentration. These data support a model in which NFI enhances initiation through an interaction with pTP-pol. Glutaraldehyde cross-linking experiments indicate contacts between pTP-pol and NFI but not NFIII/oct-1. The site of interaction is located in the NFI-BD domain.
Asunto(s)
Adenovirus Humanos/genética , Proteínas Potenciadoras de Unión a CCAAT , Proteínas de Unión al ADN/fisiología , Regulación Viral de la Expresión Génica , Proteínas Nucleares/fisiología , Factores de Transcripción/fisiología , Replicación Viral , Secuencia de Bases , Sitios de Unión , Replicación del ADN , ADN Viral/metabolismo , ADN Polimerasa Dirigida por ADN/metabolismo , Células HeLa , Factor C1 de la Célula Huésped , Técnicas In Vitro , Sustancias Macromoleculares , Datos de Secuencia Molecular , Factores de Transcripción NFI , Factor 1 de Transcripción de Unión a Octámeros , Secuencias Reguladoras de Ácidos NucleicosRESUMEN
The acyclic adenosine analogue (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [S]-HPMPA) is a potent and selective inhibitor of adenovirus (Ad) replication in cell culture. We studied the mechanism of inhibition using a reconstituted in vitro DNA replication system. The diphosphoryl derivative (S)-HPMPApp, but not (S)-HPMPA, inhibited the DNA replication of origin containing fragments strongly. The inhibitory effect was exerted at the level of elongation, while initiation was resistant to the drug. Remarkably, the elongation of short strands was only slightly impaired, while inhibition was maximal upon synthesis of long DNA fragments. (S)-HPMPApp appeared to be competitive with dATP, suggesting that the Ad DNA polymerase is the prime target for the drug. We purified the Ad DNA polymerase in complex to the precursor terminal protein to homogeneity from cells infected with overproducing recombinant vaccinia viruses. Employing gapped DNA or poly(dT).oligo(dA) templates, only a weak inhibition was observed. However, inhibition was strongly enhanced in the presence of the adenovirus DNA binding protein (DBP). We interpret this to mean that the increased processivity of the polymerization reaction in the presence of DBP leads to increased drug sensitivity.
Asunto(s)
Adenina/análogos & derivados , Adenovirus Humanos/genética , Antivirales/farmacología , Replicación del ADN/efectos de los fármacos , ADN Viral/biosíntesis , Proteínas de Unión al ADN/metabolismo , ADN Polimerasa Dirigida por ADN/aislamiento & purificación , Organofosfonatos , Compuestos Organofosforados/farmacología , Adenina/metabolismo , Adenina/farmacología , Adenovirus Humanos/efectos de los fármacos , Adenovirus Humanos/enzimología , ADN Viral/efectos de los fármacos , ADN Polimerasa Dirigida por ADN/metabolismo , Células HeLa/enzimología , Humanos , Cinética , Inhibidores de la Síntesis del Ácido Nucleico , Compuestos Organofosforados/metabolismo , Unión Proteica , Moldes Genéticos , Virus Vaccinia/genéticaRESUMEN
Two-dimensional polyacrylamide gel electrophoresis can be used to identify structural forms of RNA such as linear RNA, circular RNA, interlocked circles and lariats. The procedure is based upon the characteristic migration behaviour of the degradation products derived from the intact structures present already before the start of the experiment or formed during or after electrophoresis in the first dimension. After autoradiography to detect the positions of the radiolabeled RNA molecules, circles broken during electrophoresis of the first dimension give rise to horizontal lines touching the diagonal formed by linear RNAs at a point corresponding to the length of the RNA circle from which it was derived. Products derived from interlocked RNA circles by breakage after completion of the first dimension appear on a vertical line underneath the intact complex and consist of free RNA circles and their linear derivatives. Broken lariats give rise to two lines depending on the location of the break. Lariats with broken tails are present on a line to a position that corresponds to the length of their tail and that runs parallel to the diagonal formed by linear products. Lariats with a broken eye form a line running from the position of the intact product to the diagonal formed by the linear RNAs.