RESUMEN

A water soluble glucan of average molecular weight ∼1.74×10(5)Da was isolated from hot aqueous extract of the fruiting bodies of an ectomycorrhizal edible mushroom Tricholoma crassum (Berk.) Sacc. The structure of this glucan was elucidated on the basis of total hydrolysis, methylation analysis, Smith degradation, and 1D/2D NMR studies. Based on the above experiments the repeating unit of the glucan was established as: [formula see text]. This glucan showed macrophage activation in vitro by NO production in a dose dependent manner and strong splenocyte and thymocyte immunostimulation in mouse cell culture medium. It also exhibited good inhibition activity toward lipid peroxidation.

Asunto(s)

Glucanos/química , Tricholoma/química , Animales , Células Cultivadas , Glucanos/farmacología , Peroxidación de Lípido/efectos de los fármacos , Macrófagos/efectos de los fármacos , Espectroscopía de Resonancia Magnética , Ratones , Timocitos/efectos de los fármacos

RESUMEN

A water soluble glucan (PS-I) was isolated from the hot aqueous extract of the fruit bodies of an ectomycorrhizal edible mushroom, Russula albonigra (Krombh.) Fr. The total hydrolysis, methylation analysis, periodate oxidation, and NMR ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC) studies revealed the presence of the following repeating unit in the polysaccharide: This glucan showed excellent activation of macrophages as well as splenocytes and thymocytes in vitro.

Asunto(s)

Agaricales/química , Glucanos/química , Glucanos/farmacología , Calor , Micorrizas/química , Agua/química , Animales , Secuencia de Carbohidratos , Línea Celular , Cuerpos Fructíferos de los Hongos/química , Glucanos/aislamiento & purificación , Células HeLa , Humanos , Factores Inmunológicos/química , Factores Inmunológicos/aislamiento & purificación , Factores Inmunológicos/farmacología , Macrófagos/efectos de los fármacos , Macrófagos/inmunología , Ratones , Datos de Secuencia Molecular , Solubilidad