RESUMEN
The expression of adhesion molecules on the vascular endothelium of healthy and inflamed human dental pulp was studied immunohistochemically using antibodies for selectin family and immunoglobulin super-family members. In healthy dental pulp, some vessels reacted very weakly with major histocompatibility complex (MHC) class II, and E- and P-selectin, and many vessels reacted with platelet-endothelial cell adhesion molecule-1 (PECAM-1), but no vessels reacted with intercellular adhesion molecule-1 (ICAM-1), ICAM-3 and vascular cell adhesion molecule-1 (VCAM-1). In inflamed pulp, a large number of vessels reacted strongly with MHC class II, E- and P-selectin, PECAM-1, ICAM-1, ICAM-3, and VCAM-1. This indicates that the vascular endothelium of the inflamed human dental pulp expresses diverse adhesion molecules for leukocyte emigration from the blood stream into tissue. Dental pulp is surrounded by dentin and isolated from other tissue, like an in vitro system, so dental pulp is an attractive model for studying the roles of adhesion molecules on the vascular endothelium in transendothelial migration of leukocytes.
Asunto(s)
Antígenos CD , Antígenos de Diferenciación , Moléculas de Adhesión Celular/análisis , Movimiento Celular/fisiología , Pulpa Dental/química , Endotelio Vascular/química , Leucocitos/citología , Adolescente , Adulto , Anticuerpos , Moléculas de Adhesión Celular/inmunología , Pulpa Dental/irrigación sanguínea , Pulpa Dental/citología , Selectina E/análisis , Selectina E/inmunología , Endotelio Vascular/inmunología , Antígenos de Histocompatibilidad Clase II/análisis , Antígenos de Histocompatibilidad Clase II/inmunología , Humanos , Inmunoglobulinas/análisis , Inmunoglobulinas/inmunología , Molécula 1 de Adhesión Intercelular/análisis , Molécula 1 de Adhesión Intercelular/inmunología , Diente Molar/química , Selectina-P/análisis , Selectina-P/inmunología , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/análisis , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/inmunología , Pulpitis/inmunología , Molécula 1 de Adhesión Celular Vascular/análisis , Molécula 1 de Adhesión Celular Vascular/inmunologíaRESUMEN
Light microscopic identification of lymphatic vessels of the human small intestine was studied immuno-histochemically with a monoclonal antibody specific for human thoracic duct (mAb-D) and rabbit anti-human laminin antibody (anti-L). Two types of vessels were observed with anti-L; one strongly reacted with regularly outlined vessels and the other weakly reacted with irregularly outlined vessels and thin walls. Immunoreactivity to mAb-D was strong in the weakly reacting vessels, and the strongly reacting vessels did not react to mAb-D. Immunoreactivity of lymphatic vessels to anti-L has been reported to be weaker than that of the blood vessels; only irregularly outlined vessels which were weakly immunostained with anti-L reacted to mAb-D and no cross-reaction to mAb-D was observed in the regularly outlined vessels. This indicates that the vessels strongly reacting with anti-L are blood vessels and the vessels immunostained with mAb-D must be lymphatics. The study shows that it is possible to identify lymphatic vessels light microscopically by mAb-D. This method will be useful in studying the fine distribution of lymphatic vessels in normal tissue and also in pathological tissue such as metastasis of malignant tumors.