RESUMEN
In the present study, the polymer obtained from Cydonia oblonga Miller seeds (quince seed mucilage (QSM)) in combination with polycaprolactone (PCL) was used for producing hybrid electrospun scaffolds as three-dimensional (3D) cell culture platforms. Various PCL/QSM ratios were tested to obtain a uniform product with an appropriate mean fiber diameter for cell growth. The chemical structures of the scaffolds were studied by FT-IR spectroscopy and their crystallinity was investigated by XRD. Fiber morphology studies by SEM revealed that the fiber mean diameters decrease upon increasing the QSM component of the electrospun compound. The PCL/QSM ratio of 60/40 exhibited desirable uniform bead-free nanofibers. The hydrophilicity of the scaffolds were explored by contact angle measurements, which indicated that more hydrophilic fibers are produced as the PCL/QSM ratio is decreased. Porosity and strength of the scaffolds were also studied, which showed the key role of PCL in mechanical strength of the nanofibers. The results of cell culture experiments using epithelial Vero cells on the scaffolds displayed their high capacity in cell growth and proliferation. It was revealed that the electrospun PCL/QSM based scaffolds with 3D structures and 75-150 nm mean fiber diameters are able to maximize adhesion and growth of Vero cells.
Asunto(s)
Nanofibras/química , Mucílago de Planta/química , Poliésteres/química , Rosaceae/química , Ingeniería de Tejidos , Andamios del Tejido/química , Animales , Técnicas de Cultivo de Célula , Proliferación Celular , Chlorocebus aethiops , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Porosidad , Semillas/química , Espectroscopía Infrarroja por Transformada de Fourier , Células VeroRESUMEN
BACKGROUND: Functional knee braces are extensively used for partially and completely torn anterior cruciate ligament (ACL) patients and those who have undergone ACL graft reconstruction, in order to support the healing ACL, improve the joint's functional stability, and restore the normal joint kinematics. RESEARCH QUESTION: Does wearing braces alter the arthrokinematics of the ACL deficient knees during lung exercise? METHODS: For ten male unilateral ACL deficient subjects, 3D knee models were reconstructed from CT images, acquired in rest position. Sagittal plane fluoroscopy was then performed throughout a complete cycle of lunge in braced and non-braced conditions. The 3D kinematics of the knees were obtained using a 2D-3D registration method in which six anatomical bony landmarks on the fluoroscopic images were matched with those on the 3D models. RESULTS: No significant difference was found between the tibial anterior-posterior translations and abduction-adduction motions of the braced and non-braced knees. A significant decrease, however, was observed after bracing in the tibial internal rotation at 45° flexion during eccentric (non-braced: 5.9° (±6.7°) vs. braced: 2.4° (±7.0°); pâ¯=â¯0.045), and at 30° flexion during concentric (non-braced: 2.3° (±6.9°) vs. braced: -1.6° (±8.1°); pâ¯=â¯0.001) phases of the lunge cycle. SIGNIFICANCE: The immediate effect of knee bracing is limited to controlling the tibial rotation of the ACL deficient individuals during the lunge exercise. Hence, care should be taken in prescribing the lunge exercise for rehabilitation of ACL injured patients with high anterior-posterior knee instability, even when wearing knee braces.
Asunto(s)
Lesiones del Ligamento Cruzado Anterior/fisiopatología , Lesiones del Ligamento Cruzado Anterior/terapia , Ligamento Cruzado Anterior/fisiopatología , Fenómenos Biomecánicos/fisiología , Tirantes , Ejercicio Físico/fisiología , Rango del Movimiento Articular/fisiología , Adulto , Reconstrucción del Ligamento Cruzado Anterior , Femenino , Humanos , Interpretación de Imagen Asistida por Computador , Inestabilidad de la Articulación/fisiopatología , Articulación de la Rodilla/fisiopatología , Masculino , Persona de Mediana Edad , Cuidados Posoperatorios , Tomografía Computarizada por Rayos X , Adulto JovenRESUMEN
In this study, a polymer obtained from the basil seed mucilage (BSM) in combination with polycaprolactone (PCL), was used in the 2D scaffold production process for cell culture. First, combinations of two polymers with different ratios and concentrations were prepared and electrospun. Among these samples, a sample with a BSM/PCL ratio of 2/3 was used to perform different tests due to its fibre uniformity and appropriate diameter. The Fourier transform infrared spectrometer test was carried out to chemically analyse the scaffold, the X-ray diffraction test to determine the crystallinity of the scaffold, and the contact angle test to determine the hydrophilicity of the scaffold. The strength, porosity, and degradation percentage of the scaffold were also studied. With appropriate conditions of the scaffold for cell culture determined, Vero epithelial cells were cultured on the scaffold. Results obtained from cell culture indicated that the adhesion of the scaffold was suitable for the appropriate growth cells.