RESUMEN
We examined the genetic variability in the pig-human tapeworm, Taenia solium, by sequencing the genes for cytochrome oxidase I, internal transcribed spacer 1, and a diagnostic antigen, Ts14, from individual cysts isolated from Peru, Colombia, Mexico, India, China, and the Philippines. For these genes, the rate of nucleotide variation was minimal. Isolates from these countries can be distinguished based on one to eight nucleotide differences in the 396 nucleotide cytochrome oxidase I (COI) sequence. However, all of the 15 isolates from within Peru had identical COI sequences. The Ts14 sequences from India and China were identical and differed from the Peru sequence by three nucleotides in 333. These data indicate that there is minimal genetic variability within the species T. solium. Minimal variability was also seen in the ITS1 sequence, but this variation was observed within the individual. Twenty-two cloned sequences from six isolates sorted into 13 unique sequences. The variability observed within the sequences from individual cysts was as great as the variability between the isolates.
Asunto(s)
Antígenos Helmínticos/genética , ADN de Helmintos/genética , ADN Espaciador Ribosómico/genética , Complejo IV de Transporte de Electrones/genética , Enfermedades de los Porcinos/parasitología , Taenia/genética , Teniasis/veterinaria , Animales , Secuencia de Bases , China , Colombia , ADN de Helmintos/aislamiento & purificación , Variación Genética , Humanos , India , México , Datos de Secuencia Molecular , Perú , Filipinas , Filogenia , Homología de Secuencia de Ácido Nucleico , Porcinos , Taenia/clasificación , Teniasis/parasitologíaRESUMEN
A cintilografia esplênica seletiva pode ser o exame de eleiçäo quando há a necessidade de se estudar especificamente o baço. Para isso, descrevemos um método simplificado para marcaçäo in vitro de hemácias com tecnécio-99m com posterior desnaturaçäo térmica. Através dessa técnica é possível obter imagem cintilográfica esplênica com um número mais reduzido de manipulaçöes, quando comparada a métodos similares