RESUMEN
The polysaccharide L-II was isolated and purified from the fruiting body of Lentinus edodes, which consisted of d-glucopyranose and had the molecular weight of 2.03 x 10(5) Da. We evaluated the effects of the polysaccharide L-II on the cellular immune response of Sarcoma 180-bearing mice. Mice were treated with three doses of the polysaccharide L-II (1, 5, and 10 mg/kg body weight) for 10 days. Tumor weight, relative spleen and thymus weight, delayed-type hypersensitivity (DTH) response, phagocytosis of macrophage, splenocytes proliferation were studied. Concentration of tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) in mice serum were measured in control and polysaccharide groups. At the dose of 1, 5 and 10 mg/kg, a significant increase (p<0.05) in relative spleen and thymus weight, DTH, phagocytosis of macrophage was observed, as well as a significant decrease in tumor formation. The concentration of TNF-alpha, IFN-gamma in serum increased significantly in the polysaccharide groups compared with the model control group, but IL-2 not. Moreover, the polysaccharide L-II could increase NO production and catalase activity in macrophages. Results of these studies demonstrated the antitumor activity of the polysaccharide L-II on mice-transplanted sarcoma 180 was mediated by immunomodulation in inducing T-cells and macrophage-dependent immune system responses.
Asunto(s)
Factores Inmunológicos/aislamiento & purificación , Factores Inmunológicos/farmacología , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Hongos Shiitake/química , Animales , Catalasa/metabolismo , Citocinas/biosíntesis , Femenino , Hipersensibilidad Tardía , Factores Inmunológicos/química , Técnicas In Vitro , Activación de Linfocitos/efectos de los fármacos , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/inmunología , Macrófagos Peritoneales/metabolismo , Ratones , Peso Molecular , Óxido Nítrico/biosíntesis , Fagocitosis/efectos de los fármacos , Polisacáridos/química , Sarcoma 180/tratamiento farmacológico , Sarcoma 180/inmunología , Sarcoma 180/metabolismo , Sarcoma 180/patologíaRESUMEN
Object To study the structural characteristics of protein bond polysaccharide Le 3 from the fruitbody of Lentinus edodos (Berk ) Pegler Methods Relative molecular weights were determined by gel permeation chromatography Structural characteristics were expounded by infra red scan, gas chromatography, ? elimination reaction and Sepharose gel electrophoresis The content of total polysaccharide and protein were determined by colorimetry Results Mean molecular weight and molecular number of Le 3 were 13 700 and 12 500 Typical absorption peak of the polysaccharide as shown in infra red spectrum was of ? type glycosidic linkage Le 3 was composed of arabinose, xylose, mannose, galactose, glucose and glucuronic acid The molar ratio of the neutral saccharides was Ara∶Xyl∶Man∶Gal∶Glu=0 31∶0 47∶1 00∶1 15∶8 92 The content of total saccharide and protein were 70 62% and 25 31% The saccharides were not linked to peptides chain through O glycosidic linkage Le 3 contained RNA Conclusion Le 3 was a kind of new type polysaccharide isolated from the fruitbody of L edodes
RESUMEN
Objective:To compare the anti tumor activity and immune enhancing effect of crude(PGF) and purified (PGF 1) polysaccharide from Grifola frondosa. Methods:S 180 bearing mice were used as animal model. The effects on tumor weight and immune function were investigated. Results:PGF and PGF 1 showed inhibitory activity on S 180 growth. They could also increase weight of immune organs and improved the phagocytic function, DTH response, lymphocyte proliferation, antibody formation in splenic cells and content of serum HC IgM markedly. PGF was better than PGF 1. Conclusion:Both PGF and PGF 1 can inhibit S 180 tumor growth and enhance immune function in S 180 bearing mice.
RESUMEN
Objective: To study the inhibition effect on Sarcoma 180 (S 180 ) and immune enhancing effect of rapeseed peptides (RSP) from enzymolysis of rapeseed protein. Methods: S 180 -bearing mice were used as animal model. The changes of tumor weight, lymphocyte proliferation, antibody in splenic cells and hemolysin were investigated. Results: Compared with cyclophosphamide (Cy), RSP inhibited S 180 growth markedly and enhanced immune function .Conclusion: RSP has potential in enhancing immune function and inhibiting tumor growth, but it deserves for further study.
RESUMEN
Objective: To clarify the changes of content and composition of tocopherol in rapeseed oil during the refining process. Methods: The content and composition of tocopherol was determined by HPLC. Results: The content of tocopherol in oil is: crude oil 0.1298%, degummed and neutralized oil 0.1189 %, bleached oil 0.0825%, deodorized oil 0.0686%. Compared to crude oil, the total tocopherol content decreased 47.1% after the whole refining process,and the ?-tocopherol content decreased 33.1%,66.4%,66.7% after degumming and neutralization, bleaching, deodorization, respectively. Conclusion: The content of tocopherol and ?-tocopherol decreases significantly as well as the relative proportion of ?-tocopherol in total tocopherol after refining.These changes must be taken into account during the refining technology optimization, utilization of by-products and evaluation of oxidant stability of rapeseed oil .
RESUMEN
Objective: To study the effect of rapeseed peptides (RSP) from enzymolysis of rapeseed protein on growth of Sarcoma 180 (S180) cell incubated in vitro and its cell membrane. Methods: S180 cell incubated in vitro was used as model. The change of proliferation of S180 cell, fatty acid and sialic acid in membrane and membrane fluidity of S180 cell were investigated. Results: RSP had inhibitiory ability to S180 cell proliferation and could influence its membrane. Conclusion: RSP had potential in inhibiting tumor growth, so was needed to study further.