RESUMEN
Widespread lead (Pb) contamination of agricultural soils is a global issue stemming from human activities. The remediation of Pb-contaminated soils used for agricultural purposes is critically important to safeguard food crop safety. Despite the modulating effects of sulfur (S) on plant responses to toxic heavy metals, the ecological, physiological, and molecular mechanisms driving such modulation in the Pb hyperaccumulator Arabis alpina L. remain unclear. Here, we investigated the effects of five S concentrations (0, 50, 100, 150, and 200 mg kg-1) on A. alpina grown in Pb-contaminated soil from a lead-zinc mining area. Under S50 (i.e., 50 mg kg-1) and S100 treatments, the Pb concentration in both shoots and roots of A. alpina significantly decreased compared to the control (S0). Specifically, the S50 treatment significantly enhanced Pb accumulation, plant biomass, and plant height, indicating that low S applications facilitate Pb accumulation from the soil and alleviate Pb toxicity. Additionally, S50, S100, and S150 treatments significantly improved photosynthetic rate, stomatal conductance, and intercellular CO2 concentration in A. alpina. Transcriptomic analysis showed that S50 and S100 treatments increased the expression of the LHCA, LHCB, psa, and psb genes, which had a significant impact on photosynthetic efficiency. S50 and S100 boosted glutathione (GSH) levels in A. alpina roots, and the increased expression of GST gene enhanced tolerance to environmental stress. In summary, these results suggest that an appropriate supply of S (S50 and S100) not only alleviates Pb toxicity by enhancing plant biomass, height, photosynthetic features, and sulfur metabolites but also stimulates Pb accumulation in the hyperaccumulator A. alpina. Our study elucidated the specific concentrations of sulfur that optimally enhance both Pb accumulation and stress tolerance in the hyperaccumulator A. alpina, providing novel insights into the practical application of sulfur in phytoremediation strategies and advancing our understanding of the underlying molecular mechanisms.
Asunto(s)
Arabis , Biodegradación Ambiental , Plomo , Contaminantes del Suelo , Azufre , Plomo/toxicidad , Plomo/metabolismo , Contaminantes del Suelo/toxicidad , Contaminantes del Suelo/metabolismo , Arabis/metabolismo , Arabis/efectos de los fármacos , Fotosíntesis/efectos de los fármacos , Suelo/química , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , MineríaRESUMEN
In order to explore the utilization of Eucalyptus sawdust (C) and develop its remediation potential in cadmium and arsenic contaminated soil, Eucalyptus sawdust were modified by FeCl3 and NaOH coprecipitation (MC). Characterization technology and pot experiment were used to explore the adsorption mechanism of cadmium and arsenic by MC and the effect of soil remediation. The results showed that iron oxide was loaded on the surface of Eucalyptus sawdust and destroyed the semi fiber structure. The adsorption mechanisms of cadmium and arsenic included electrostatic attraction, precipitation, complexation, redox. The soil pH value reduced by 0.12-0.18 units with 0.25%-1% ratio of application rates of MC to soil weight treatment; The contents of available cadmium and arsenic were reduced by 18%-25% and 12%-18%; MC could promote the transformation of Cd and As from highly active formation to low active formation and had a good application prospect for Cd and As compound pollution remediation.