RESUMEN
Papain, a phytotherapeutic agent, has been used in the treatment of eschars and as a debriding chemical agent to remove damaged or necrotic tissue of pressure ulcers and gangrene. Its benefits in these treatments are deemed effective, since more than 5000 patients, at the public university hospital at Rio de Janeiro, Brazil, have undergone papain treatment and presented satisfactory results. Despite its extensive use, there is little information about toxic and mutagenic properties of papain. This work evaluated the toxic and mutagenic potential of papain and its potential antioxidant activity against induced-H(2)O(2) oxidative stress in Escherichia coli strains. Cytotoxicity assay, Growth inhibition test, WP2-Mutoxitest and Plasmid-DNA treatment, and agarose gel electrophoresis were used to investigate if papain would present any toxic or mutagenic potential as well as if papain would display antioxidant properties. Papain exhibited negative results for all tests. This agent presented an activity protecting cells against H(2)O(2)-induced mutagenesis.
RESUMEN
Stannous chloride (SnCl(2)) and UVA induce DNA lesions through ROS. The aim of this work was to study the toxicity induced by UVA preillumination, followed by SnCl(2) treatment. E. coli BER mutants were used to identify genes which could play a role in DNA lesion repair generated by these agents. The survival assays showed (i) The nfo mutant was the most sensitive to SnCl(2); (ii) lethal synergistic effect was observed after UVA pre-illumination, plus SnCl(2) incubation, the nfo mutant being the most sensitive; (iii) wild type and nfo mutants, transformed with pBW21 plasmid (nfo(+)) had their survival increased following treatments. The alkaline agarose gel electrophoresis assays pointed that (i) UVA induced DNA breaks and fpg mutant was the most sensitive; (ii) SnCl(2)-induced DNA strand breaks were higher than those from UVA and nfo mutant had the slowest repair kinetics; (iii) UVA + SnCl(2) promoted an increase in DNA breaks than SnCl(2) and, again, nfo mutant displayed the slowest repair kinetics. In summary, Nfo protects E. coli cells against damage induced by SnCl(2) and UVA + SnCl(2).
Asunto(s)
Daño del ADN/fisiología , Reparación del ADN/efectos de los fármacos , Reparación del ADN/efectos de la radiación , ADN/efectos de los fármacos , ADN/efectos de la radiación , Desoxirribonucleasa IV (Fago T4-Inducido)/metabolismo , Proteínas de Escherichia coli/metabolismo , Escherichia coli , Compuestos de Estaño/administración & dosificación , Escherichia coli/efectos de los fármacos , Escherichia coli/enzimología , Escherichia coli/efectos de la radiación , Especificidad de la Especie , Rayos UltravioletaRESUMEN
Aloe vera is a tropical plant, known in Brazil as babosa and several reputable suppliers produce a stabilized aloe gel for topic use. Since people use Aloe vera topically, they could be exposed to solar ultraviolet light in addition and it might cause a cross damage effect between these agents. The aim of this work was to investigate the biological effects of Aloe vera pulp extract, associated or not to UVA radiation, on Escherichia coli-deficient repair mutants and plasmid DNA, in order to test its genotoxic potential. Data obtained from analysis of survival fractions, bacterial transformation and agarose gel electrophoresis suggest that Aloe vera has genotoxic properties, but it seems not to be able to damage the cell membrane.