RESUMEN
The effect of Nw-nitro-L-arginine on embryonic implantation and cGMP carbonyl group concentration was assessed at the rat implantation site (IS) and non-implantation site (NIS). The intraluminal administration of 25 microg (2.3 mM) of Nw-nitro-L-arginine inhibited implantation in 34.7% and embryo survival (100%), while in addition, decreasing cGMP concentration both at the site (1664.2 +/- 333.8 pmoles/mg of protein for the control and 1321 +/- 384.3 for those treated), as well as at the NIS (1203.7 +/- 200 to 780.2 +/- 168.5). Carbonyl group concentration was considerably less at the implantation site treated with Nw-nitro-L-arginine than in the control (0.062 +/- 0.012 nmoles/mg of protein and 0.45 +/- 0.1, respectively). Nonetheless, the NIS was not significantly different (0.12 +/- 0.04 and 0.15 +/- 0.05). Our results show that a nitric oxide (NO) dependent system parallel to the formation of cGMP and protein peroxidation products is important at the blastocyst implantation site in order for the endometrium to acquire the necessary properties for an adequate receptivity.
Asunto(s)
GMP Cíclico/metabolismo , Implantación del Embrión/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , Óxido Nítrico Sintasa/antagonistas & inhibidores , Óxido Nítrico/biosíntesis , Preñez/metabolismo , Animales , AMP Cíclico/biosíntesis , Desarrollo Embrionario y Fetal/efectos de los fármacos , Endometrio/efectos de los fármacos , Endometrio/enzimología , Femenino , Indicadores y Reactivos , Nitroarginina/farmacología , Oxidación-Reducción , Peróxidos/metabolismo , Fenilhidrazinas , Embarazo , Proteínas/metabolismo , Ratas , Ratas Sprague-DawleyAsunto(s)
Encéfalo/metabolismo , Ácido gamma-Aminobutírico/fisiología , Canales de Calcio/fisiología , Cloruros/metabolismo , Canales Iónicos/efectos de los fármacos , Canales Iónicos/fisiología , Potenciales de la Membrana , Fármacos Neuroprotectores/farmacología , Progesterona/metabolismo , Receptores de GABA-A/efectos de los fármacos , Receptores de GABA-A/metabolismo , Receptores de GABA-B/efectos de los fármacos , Receptores de GABA-B/metabolismo , Sodio/metabolismo , Agua/metabolismoRESUMEN
Three specifically made computational programs are described. These programs were developed in order to delimitate and analyze the accelerometric signals generated in relation to the pelvic thrusting performed by the male rat during its mounting, intromission, and ejaculation copulatory responses. This methodology has allowed us to perform quantitative analysis, not only about duration and frequency but also about amplitude or vigor of pelvic thrusting trains. We found that during long ejaculation, the extravaginal pelvic movement had a significantly higher amplitude than that of the intravaginal movements.