RESUMEN
The influence of probiotic strains of Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animalis VKL, Lactobacillus delbrueckii subsp. bulgaricus IMV B-7281 and Bifidobacterium animalis VKB, each strain separately, to the range of the urogenital tract microflora in physiological norm and in cases of experimental intravaginal staphylococcosis of mice induced by Staphylococcus aureus 8325-4. It was found that all these strains had different efficiency in Staphylococcus aureus 8325-4 growth suppression in the vagina of infected mice and affect the spectrum of microorganisms. Lactobacillus casei IMV B-7280 strain had effective antistaphylococcal effect and reduced the number of coliform bacteria and fungi in the vagina in cases of intravaginal staphylococcosis of mice. Lactobacillus casei IMV B-7280 strain is promising to create probiotic drugs effective in treating intravaginal staphylococcosis.
Asunto(s)
Probióticos/uso terapéutico , Infecciones Estafilocócicas/tratamiento farmacológico , Vagina/microbiología , Vaginosis Bacteriana/tratamiento farmacológico , Administración Intravaginal , Animales , Antibiosis , Recuento de Colonia Microbiana , Modelos Animales de Enfermedad , Femenino , Ratones , Ratones Endogámicos BALB C , Probióticos/administración & dosificación , Probióticos/farmacología , Infecciones Estafilocócicas/microbiología , Vagina/efectos de los fármacos , Vaginosis Bacteriana/microbiologíaRESUMEN
Antistaphylococcal action of Lactobacillus delbrueckii subsp. bulgaricus IMV B-7281, L. acidophilus LMV B-7279 and Bifidobacterium animalis VKB as well as interleukin-2 (IL-2) has been determined on the model of experimental staphylococcal infection in mice. It has been established that peroral administration of certain probiotic strains of lacto- and bifidobacteria or composition L. acidophilus LMV B-7279--L. delbrueckii subsp. bulgaricus IMV B-7281 with IL-2 to mice favored the reducing of terms of staphylococcus persistence in the kidneys of experimental mice. The studied schemes are promising for treatment of patients with surgical infection.
Asunto(s)
Bifidobacterium/fisiología , Interleucina-2/farmacología , Lactobacillus/fisiología , Probióticos/farmacología , Infecciones Estafilocócicas/tratamiento farmacológico , Staphylococcus aureus/efectos de los fármacos , Animales , Recuento de Colonia Microbiana , Terapia Combinada , Inyecciones Intraperitoneales , Riñón/efectos de los fármacos , Riñón/microbiología , Ratones , Ratones Endogámicos BALB C , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/crecimiento & desarrolloRESUMEN
The effect of intravaginal injection of Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animalis VKL and Bifidobacterium animalis VKB strains in various compositions on the range of microflora of the urogenital tract on the model of experimental intravaginal staphylococcosis of mice was determined. It was established that under the influence of various compositions of these strains changes in microflora spectrum occurred: the number of representatives of normoflora increased and the number of pathogenic microorganisms, including staphylococci, streptococci, coliform bacteria and fungi, significantly decreased. It was determined that strains of lacto- and bifidobacteria, that were studied, are prospective components of future probiotic drugs efficient in treating staphylococcosis.
Asunto(s)
Bifidobacterium/fisiología , Lactobacillus/fisiología , Probióticos/farmacología , Infecciones Estafilocócicas/prevención & control , Staphylococcus aureus/efectos de los fármacos , Vagina/microbiología , Administración Intravaginal , Animales , Antibiosis , Recuento de Colonia Microbiana , Modelos Animales de Enfermedad , Femenino , Humanos , Ratones , Ratones Endogámicos BALB C , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/crecimiento & desarrollo , Staphylococcus aureus/patogenicidad , Vagina/efectos de los fármacosRESUMEN
The effect of intravaginal injection of Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animalis VKL and Bifidobacterium animalis VKB strains in various compositions on the range of microflora of the urogenital tract on the model of experimental intravaginal staphylococcosis of mice was determined. It was established that under the influence of various compositions of these strains changes in microflora spectrum occurred the number of representatives of normoflora increased and the number of pathogenic microorganisms, including staphylococci, streptococci, coliform bacteria and fungi, significantly decreased. It was determined that strains of lacto- and bifidobacteria, that were studied, are prospective components of future probiotic drugs efficient in treating staphylococcosis.
Asunto(s)
Bifidobacterium/fisiología , Lacticaseibacillus casei/fisiología , Lactobacillus acidophilus/fisiología , Probióticos/uso terapéutico , Infecciones Estafilocócicas/terapia , Vagina/efectos de los fármacos , Administración Intravaginal , Animales , Antibiosis , Recuento de Colonia Microbiana , Modelos Animales de Enfermedad , Enterobacteriaceae/efectos de los fármacos , Enterobacteriaceae/crecimiento & desarrollo , Femenino , Hongos/efectos de los fármacos , Hongos/crecimiento & desarrollo , Humanos , Ratones , Ratones Endogámicos BALB C , Probióticos/administración & dosificación , Infecciones Estafilocócicas/microbiología , Staphylococcus/efectos de los fármacos , Staphylococcus/crecimiento & desarrollo , Streptococcus/efectos de los fármacos , Streptococcus/crecimiento & desarrollo , Vagina/microbiologíaRESUMEN
The immunomodulatory properties of Lactobacillus delbrueckii subsp. bulgaricus IMVB-7281, Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animales VKL and B. animales VKB strains on the models of experimental staphylococcosis infection in mice were determined. It was found that after the mice, infected with staphylococcus, were treated by some probiotic strains of lacto- and bifidobacteria, a normalization of functional activity of phagoctic cells system and increase of the endogenous interferon production were observed. L. delbrueckii subsp. bulgaricus IMVB-7281, L. casei IMVB-7280, L. acidophilus IMV B-7279, B. animales VKB and B. animales VKL are promising for the development of probiotics, effective against staphylococci and for the immunity correction.
Asunto(s)
Bifidobacterium/fisiología , Lacticaseibacillus casei/fisiología , Lactobacillus acidophilus/fisiología , Macrófagos Peritoneales/efectos de los fármacos , Probióticos/uso terapéutico , Infecciones Estafilocócicas/inmunología , Administración Oral , Animales , Antibiosis , Recuento de Células , Femenino , Inmunomodulación/efectos de los fármacos , Inyecciones Intraperitoneales , Interferones/biosíntesis , Interferones/inmunología , Células Asesinas Naturales/efectos de los fármacos , Células Asesinas Naturales/inmunología , Macrófagos Peritoneales/inmunología , Ratones , Ratones Endogámicos BALB C , Peritoneo/efectos de los fármacos , Peritoneo/inmunología , Peritoneo/microbiología , Probióticos/administración & dosificación , Bazo/efectos de los fármacos , Bazo/inmunología , Bazo/microbiología , Infecciones Estafilocócicas/microbiología , Infecciones Estafilocócicas/terapia , Staphylococcus/efectos de los fármacosRESUMEN
The results of research on changes in the level of apoptosis among the spleen cells during the development of intravaginal staphylococcal infection in experimental models of different genetic mice lines are given in the paper. Intravaginal injection of Staphylococcus aureus resulted in an increased number of cells with morphological characteristics of apoptosis in underbred and line BALB/c mice, while it decreased in the ICR line mice. The level of the apoptotic cells in the underbred and line BALB/c mice was accompanied by some level of microorganisms that were seeded out of kidneys--the higher was the number of microorganisms, the more apoptotic cells were indicated.