RESUMEN

Under suboptimal environmental conditions such as low temperatures, many bacteria have an extended lag phase, altered cell structures, and composition such as a less fluid (more rigid) and leaky cytoplasmic membrane. As a result, cells may die, enter into a starvation mode of metabolism or a physiologically viable but non-culturable (VBNC) state. In the latter state, the amount of gene expression per cell is virtually undetectable. In this article, gene expression under (suboptimal) low temperature conditions in non-psychrophilic environmental bacteria is examined. The pros and cons of some of the molecular methodologies for gene expression analysis are also discussed.

Asunto(s)

Bacterias/genética , Regulación Bacteriana de la Expresión Génica , Genes Bacterianos , Secuencia de Aminoácidos , Regiones Antárticas , Proteínas Anticongelantes/metabolismo , Proteínas Bacterianas/genética , Frío , Electroforesis en Gel Bidimensional/métodos , Congelación , Viabilidad Microbiana/genética , Datos de Secuencia Molecular , Análisis de Secuencia por Matrices de Oligonucleótidos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Homología de Secuencia de Aminoácido