RESUMEN
Insect-borne diseases remain to this day a major source of illness and death worldwide. The resistance to chemical insecticides among mosquito species has been considered as a setback in vector control. Mosquito control programs, botanical origin may have the potential to eliminate eggs and larvae. So, the larvicidal and ovicidal activities of crude benzene, hexane, ethyl acetate, chloroform and methanol extracts of Terminalia chebula were assayed for their toxicity against three important vector mosquitoes, viz., Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. The larval mortality was observed after 24 h of exposure. All extracts showed moderate larvicidal effects; however, the highest larval mortality was found in the methanol extract of T. chebula against the larvae of A. stephensi, A. aegypti, and C. quinquefasciatus with the LC50 values were 87.13, 93.24 and 111.98 ppm, respectively. Mean percent hatchability of the ovicidal activity was observed 48 h post treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. All the five solvent extracts showed moderate ovicidal activity; however, the maximum egg mortality (zero hatchability) was observed in the methanol extract of T. chebula at 200 and 250 ppm against A. stephensi, A. aegypti and C. quinquefasciatus showed 100% mortality at 300 ppm. No mortality was recorded in the control. The finding of the present investigation revealed that the leaf extract of Terminalia chebula possesses remarkable larvicidal and ovicidal activity against medically important vector mosquitoes and make this plant product promising as an alternative to synthetic insecticide in mosquito control programs.
RESUMEN
To identify the larvicidal activities of silver nanoparticles synthesised with Avicennia marina leaf extract against the larvae of Aedes aegypti and Anopheleus stephensi, in vitro larvicidal activities such as LC50 and LC90 were assessed. Further, characterisation such as UV and FTIR analysis were carried out for the synthesised silver nanoparticles. The LC50 value of the synthesised silver nanoparticles was identified as 4.374 and 7.406 mg/L for An. stephensi and Ae. aegypti larvae respectively. Further, the LC90 values are also identified as 4.928 and 9.865 mg/L for An. stephensi and Ae. aegypti species respectively. The synthesised silver nanoparticles have maximum absorption at 420 nm with the average size of 60-95 nm. The FTIR data showed prominent peaks in (3940.57, 3929.00, 3803.63, 3712.97, 2918.30, 2231.64, 1610.50, 1377.17, 1257.59, 1041.59, 1041.56, 775.38, 667.37 and 503.21) different ranges. The biosynthesis of silver nanoparticles with leaf aqueous extract of A. marina provides potential source for the larvicidal activity against mosquito borne diseases. The present study proved the mosquitocidal properties of silver nanoparticles synthesised from mangroves of Vellar estuary. This is an ideal eco-friendly approach for the vector control programs.