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This research paper focuses on exploring the possibility of delivering macro, micro and trace elements using seed encapsulation through nano-fibres that are known to improve the nutrient use efficiencies while reducing the loss of nutrients. The nano-fibres were developed using an electrospinning machine by subjecting the polymer solution (10% polyvinyl alcohol PVA) loaded with recommended quantities of nutrients under optimal solution (pH, concentration, viscosity) and process (voltage, flow rate, tip-to-collector distance) parameters. The nano-fibres were characterized using SEM, TEM, FT-IR, XRD, TGA and Impedance spectra besides nutrient release pattern by ICP-MS. The data have clearly shown that nano-fibres retained nutrients and released slowly up to 35 days. After the characterization, green gram (Vigna radiata L) seeds were encapsulated with nano-fibres loaded with multi-nutrients and each seed was coated with approximately 20-25 mg of nano-fibres, dibbled into the soil and the physiological, nutritional, growth and yield responses were assessed. Seeds encapsulated with nano-fibres fortified with nutrients (NF) had registered significantly higher crop emergence percentage (C 62%; NF 99.8%), root length (C 12.3; NF 27.1 cm), shoot length (C 28.7; NF 47.7 cm), dry matter production (C 16.2; NF 27.5 g) and grain yield (C 621.6; NF 796.3 kg ha-1). All the parameters measured in nano-fibre encapsulated seeds fortified with 100% of recommended dose of nutrients (NF) were higher than uncoated control (C) seeds but comparable with 100 % conventional fertilizer applied ones (RDF). Such phenomenal increase in growth and yield parameters associated with the extensive surface area of nano-fibres that is capable of retaining and releasing nutrients in a regulated pattern and assist in improving the pulses productivity by achieving balance crop nutrition which alleviating multi-nutrient deficiencies.

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Microplastic particles ubiquitously found in the environment are ingested by a huge variety of organisms. Subsequently, microplastic particles can translocate from the gastrointestinal tract into the tissues likely by cellular internalization. The reason for cellular internalization is unknown, since this has only been shown for specifically surface-functionalized particles. We show that environmentally exposed microplastic particles were internalized significantly more often than pristine microplastic particles into macrophages. We identified biomolecules forming an eco-corona on the surface of microplastic particles, suggesting that environmental exposure promotes the cellular internalization of microplastics. Our findings further indicate that cellular internalization is a key route by which microplastic particles translocate into tissues, where they may cause toxicological effects that have implications for the environment and human health.

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The demand for high-quality graphene for electronic applications is increasing due to its high carrier mobility and electrical conductivity. In this connection, printing technology is a reliable method towards the fabrication of conductive, disposable graphene-based electrode for low-cost sensor application. Herein, we aimed to report the synthesis of high-quality graphene nanosheets obtained by electrochemical exfoliation of biomass-derived from corn cob. The conductive ink was prepared from this exfoliated graphene and was utilized for the preparation of paper-based graphene electrode towards double stranded DNA (dsDNA) sensor application. This paper, based graphene electrode opens the possibility of direct electrochemical analysis of analyte without any sample preparation. In this study, two irreversible oxide peaks were obtained from paper-based printed graphene electrode, corresponds to oxidation of guanine (G) and adenine (A) of dsDNA in the linear range of 0.2 pg mL-1 to 5 pg mL-1 with the detection limit of 0.68 pg mL-1 and the sensitivity of 0.00656 mA pg-1 cm-2. Further, a small-scale printable circuit is fabricated using this graphene shows good conductivity of 1.145x103(S/m).

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The anisotropic thermal expansion properties of an organic semiconducting single crystal constituted by 4-hydroxycyanobenzene (4HCB) have been probed by XRD in the range 120-300 K. The anisotropic thermal expansion coefficients for the three crystallographic axes and for the crystal volume have been determined. A careful analysis of the crystal structure revealed that the two different H-bonds stemming from the two independent, differently oriented 4HCB molecules composing the unit cell have different rearrangement patterns upon temperature variations, in terms of both bond length and bond angle. Linearly Polarized Mid InfraRed (LP-MIR) measurements carried out in the same temperature range, focused on the O-H bond spectral region, confirm this finding. The same LP-MIR measurements, on the basis of a semi-empirical relation and of geometrical considerations and assumptions, allowed calculation of the -CNH-O- hydrogen bond length along the a and b axes of the crystal. In turn, the so-calculated -CNH-O- bond lengths were used to derive the thermal expansion coefficients along the corresponding crystal axes, as well as the volumetric one, using just the LP-MIR data. Reasonable to good agreement with the same values obtained from XRD measurements was obtained. This proof-of-principle opens interesting perspectives about the possible development of a rapid, low cost and industry-friendly assessment of the thermal expansion properties of organic semiconducting single crystals (OSSCs) involving hydrogen bonds.

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CONTEXT: The psychosocial stress has long been regarded as a significant pre-disposing factor for periodontal disease. The association between the periodontal disease and the neuroendocrine hormones has been observed. Chromogranin A (CgA) is supposed to link the activity of the neuroendocrine system to local and systemic immune functions and to be related to periodontitis. AIMS: The aim of this study was to determine the CgA levels in saliva and plasma in periodontal health and disease and to assess their potential relationship to periodontitis. SETTINGS AND DESIGNS: In this case-control study, the association between periodontal disease and stress marker has been assessed. MATERIALS AND METHODS: SIXTY SUBJECTS WERE CHOSEN FOR THIS STUDY: With case group comprising of 30 subjects with chronic periodontitis and control group comprising of 30 healthy subjects. Salivary and plasma CgA levels were determined by ELISA technique. Clinical parameters included were plaque index, papillary bleeding index and clinical attachment loss and probing depth. Correlation analysis was calculated by independent sample t-test. RESULTS: Significantly higher CgA levels were found in saliva and plasma of patients with chronic periodontitis compared with healthy individuals (P < 0.05). No significant difference were observed between salivary and plasma CgA levels. CONCLUSIONS: The elevated level CgA in the plasma and saliva of subjects with stress induced chronic periodontitis has yielded insights into biological plausible association between the psychosocial stress and chronic periodontitis. Thus, our results suggest that CgA is a useful biomarker for evaluating at least in part the etiopathogenesis of periodontitis.

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The wear and mechanical properties of GUR 1020 (Perplas IMP 2000-2) Ultra High Molecular Weight Polyethylene (UHMWPE) subjected to gamma-irradiation in an atmosphere of acetylene, were evaluated for a range of processing conditions of irradiation, annealing and ageing. The results were compared with those obtained for the virgin UHMWPE material and material processed using conventional gamma-irradiation in nitrogen. Cross-linking produced by irradiation in acetylene, followed by subsequent annealing was found to be significantly more effective in improving the mechanical and wear properties of UHMWPE compared to when the material was irradiated in nitrogen. Gel fraction analysis on its own, while being able to detect the degree of cross-linking, was found to be insufficient in determining the effectiveness of the cross-links and the resulting mechanical properties of the UHMWPE material. The results suggest that gamma-irradiation in an atmosphere of acetylene may provide significant advantages over conventional UHMWPE processing and irradiation cross-linking techniques.

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