RESUMEN
In the present study, an attempt has been made to explore the antifouling potential of bioactive compound isolated from sponge associated bacterium Halobacillus kuroshimensis SNSAB01. The crude extract of SNSAB01 strongly inhibited the growth of fouling bacterial strains with least minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The bioactive compound was characterized through FT-IR, HPLC, GCMS and NMR predicted as 'pyrrolo". From the mass spectral library, structure was elucidated as pyrrolo [1, 2-a] pyrazine-1, 4-dione, hexahydro. The in silico studies provided encouraging docking scores with two interactions by GLN 200 and GLU 304. The extract inhibited 89% diatom adhesion at 350 µg/ml concentration against Amphora sp. An EC50 value of 150 µg/ml for 50% inhibition of byssal thread of Perna viridis and LC50 was found to be 500 µg/ml. The LC50/EC50 ratio of 3.0 indicated nontoxic to nature. The result suggested that pyrrolo[1,2-a]pyrazine-1,4-dione can be used for antifouling coating.
Asunto(s)
Antiinfecciosos/farmacología , Bivalvos/efectos de los fármacos , Diatomeas/efectos de los fármacos , Halobacillus/química , Pirazinas/farmacología , Pirroles/farmacología , Acetilglucosamina/análogos & derivados , Acetilglucosamina/metabolismo , Animales , Antiinfecciosos/química , Antiinfecciosos/aislamiento & purificación , Sitios de Unión , Bivalvos/crecimiento & desarrollo , Mezclas Complejas/química , Diatomeas/crecimiento & desarrollo , Matriz Extracelular/química , Halobacillus/clasificación , Pruebas de Sensibilidad Microbiana , Simulación del Acoplamiento Molecular , Filogenia , Poríferos/microbiología , Pirazinas/química , Pirazinas/aislamiento & purificación , Pirroles/química , Pirroles/aislamiento & purificación , Simbiosis/fisiologíaRESUMEN
Marine ecosystem comprises of microorganisms, plants, invertebrates and vertebrates which were rich source of diverse antimicrobial products, which were structurally unique belonging to a known class of macromolecules like peptides, terpenes, alkaloids and proteins, etc. Natural macromolecules from marine ecological niches are a promising source of antibacterial agents against several drug resistant strains of pathogenic microorganisms; whereas rest of the metabolites were derived from marine flora and fauna while some arise from microbes associated with living organisms. >30,000 natural macromolecules have been identified and reported from marine organisms, however only few macromolecules are being explored and validated. The discovery of marine antibacterial macromolecules plays a significant part in the field of drug discovery and biomedical research. Despite the fact that literatures were documented on the antifungal, antiviral, antimalarial and anticancer properties, this review exclusively highlights the different antibacterial natural macromolecules from marine sources like bacteria, fungi, sponge, algae, bryozoans, tunicates, corals, cnidarians, arthropods and echinoderm along with their mode of action.