RESUMEN
Breast carcinoma ranks as the second most common cancer among women worldwide. Despite significant therapeutic advancements, approximately 25% of breast carcinoma cases have resistance to current treatment modalities, posing a significant challenge for patient management. This study aimed to investigate the role of Sam68 mRNA and its protein in promoting oncogenesis and breast cancer progression. Sam68 protein levels were assessed in tissue samples using an Enzyme-Linked Immunosorbent Assay kit from Sun Long Biotech. Whole RNA was isolated from malignant breast tissue samples obtained from patients. The RNA concentration was determined using an Eppendorf photometer, yielding an average concentration of 62.1±10.07 ng/µl. The purity of the isolated RNA was evaluated by measuring the A260/A280 ratio (1.9±0.07) and the A260/A230 ratio (1.7±0.3). The results indicated a significant upregulation of Sam68 mRNA expression in breast cancer tissues, supporting the findings from previous studies and indicating the correlation between altered Sam68 expression and the development of breast carcinoma, highlighting the potential significance of Sam68 in the pathogenesis of the disease. Estimating Sam68 in the blood may serve as a potential biomarker for assessing the malignant grade and metastatic spread of breast carcinoma cells.
Asunto(s)
Neoplasias de la Mama , Humanos , Femenino , Neoplasias de la Mama/genética , Neoplasias de la Mama/patología , Línea Celular Tumoral , Proteínas Adaptadoras Transductoras de Señales/genética , Proteínas Adaptadoras Transductoras de Señales/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARNRESUMEN
OBJECTIVE: The aim: We investigate IGF2BP2 gene polymorphisms (rs6777038 and rs6444082) association with T2DM of Iraqi sample. PATIENTS AND METHODS: Materials and methods: The study involves 800 participants that divided to a healthy control group (400) and T2DM patients (400). Fasting blood sugar (FBS), triglycerides (Tgs), high-density lipoprotein cholesterol (HDL-Ch), total cholesterol (T-Ch), low-density lipoprotein cholesterol (LDL-Ch), and fasting insulin measured for both participant groups. IGF2BP2 gene has been genotyped for polymorphisms, rs6777038 and rs6444082 using the PCR-RFLP technique. RESULTS: Results: Logistic regression analysis testing for rs6777038 revealed that the genotype and allele frequency differ significantly (p=0.009) between T2DM and control group. In codominant model, TT genotype carriers had higher risks for diabetes than control also in the recessive model TT genotype significantly had higher risk for diabetes than control group. The other models of rs6777038 failed to reveal significant differences. The rs6777038 genotypes as codominant model showed significant differences with phenotypic characters of BMI, insulin and HOMA-IR. As well as, this SNP as dominant model showed significant differences with fasting insulin and HOMA-IR. However, rs6444082 genotypes only as dominant model reveal significant variation with HOMA-IR. CONCLUSION: Conclusions: This study confirmed the variant rs6777038 of IGF2BP2 possibly associated with T2DM risks and some anthropometric parameters such as lower fasting insulin, HOMA-IR and BMI in Iraqi T2DM participants.