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1.
Opt Express ; 18(12): 12185-90, 2010 Jun 07.
Artículo en Inglés | MEDLINE | ID: mdl-20588342

RESUMEN

Surface plasmon-coupled emission from shaped PMMA films doped with randomly oriented fluorescence molecules was investigated. Experimental results show that for different shapes, such as triangle or circular structures, the SPCE ring displays different intensity patterns. For a given shape, it was observed that the relative position and polarization of an incident laser spot on the shaped PMMA can be used to adjust the fluorescence intensity distribution of the SPCE ring. The proposed method enables controlling the fluorescence emission in azimuthal direction in addition to the radial angle controlled by common SPCE, which will further enhances the fluorescence collection efficiency and has applications in fluorescence sensing, imaging and so on.

2.
Opt Lett ; 34(7): 971-3, 2009 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-19340188

RESUMEN

Surface plasmons excited by a focused femtosecond radially polarized beam on a metal surface form a standing wave pattern with a sharp peak that can be used as a "virtual probe" for surface plasmon microscopy. The rotational symmetry of radially polarized light effectively provides the TM polarization required for coupling to the surface plasmons while the short pulse nature of the probe allows for nonlinear processes to be studied.


Asunto(s)
Microscopía Fluorescente/instrumentación , Microscopía Fluorescente/métodos , Microscopía de Polarización/instrumentación , Resonancia por Plasmón de Superficie/instrumentación , Resonancia por Plasmón de Superficie/métodos , Simulación por Computador , Diseño de Equipo , Oro/química , Luz , Metales/química , Microscopía de Polarización/métodos , Modelos Estadísticos , Fotones , Hojas de la Planta , Refractometría/instrumentación , Propiedades de Superficie , Factores de Tiempo
3.
Opt Express ; 16(25): 20734-41, 2008 Dec 08.
Artículo en Inglés | MEDLINE | ID: mdl-19065212

RESUMEN

We demonstrate the proof-of-concept for surface plasmon resonance sensing and imaging via a virtual probe at the cell-substrate interface of a biological cell in aqueous media. The technique is based on the optical excitation by focused radially polarized beams of localized surface plasmons, which forms a virtual probe on the metal substrate. The intensity distribution at the back focal plane of the objective lens enables quantitative measurements to be made of the cell-substrate contact. The acquired data is then visualized in the form of a local refractive index map.


Asunto(s)
Membrana Celular/ultraestructura , Interpretación de Imagen Asistida por Computador/métodos , Microscopía Confocal/métodos , Refractometría/métodos , Resonancia por Plasmón de Superficie/métodos
4.
Appl Opt ; 46(30): 7544-51, 2007 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-17952194

RESUMEN

A laser beam with circular polarization can be converted into either radial or azimuthal polarization by a microfabricated spiral phase plate and a radial (or azimuthal)-type linear analyzer. The resulting polarization is axially symmetric and is able to produce tightly focused light fields beyond the diffraction limit. We describe in detail the theory behind the technique and the experimental verification of the polarization both in the far field and at the focus of a high numerical aperture lens. Vector properties of the beam under strong focusing conditions were observed by comparing the fluorescence images corresponding to the focal intensity distribution for both radial and azimuthal polarizations. The technique discussed here may easily be implemented to a wide range of optical instruments and devices that require the use of tightly focused light beams.


Asunto(s)
Óptica y Fotónica , Algoritmos , Simulación por Computador , Diseño de Equipo , Luz , Microscopía , Modelos Estadísticos , Modelos Teóricos , Fotones , Espectrometría de Fluorescencia/métodos
5.
Appl Opt ; 45(6): 1153-61, 2006 Feb 20.
Artículo en Inglés | MEDLINE | ID: mdl-16523777

RESUMEN

We propose using a solitary kinoform-type spiral phase plate structure to generate an array of vortices located in a single beam. Kinoform-type spiral surfaces allow each wavelength component of the phase modulation value to be wrapped back to its 2 pi equivalent for optical vortices of high charge. This allows the surface-relief profiles of high-charge vortices to be microfabricated with the same physical height as spiral phase plates of unity-charged optical vortices. The m-charged optical vortex obtained interacts with the inherent coherent background, which changes the propagation dynamics of the optical vortex and splits the initial m charge into /m/ unity-charged optical vortices within the same beam. Compared to a hologram, a multistart spiral phase plate is more efficient in the use of available spatial frequencies and beam energy and also is computationally less demanding. Furthermore, using microfabrication techniques will allow for greater achievable tolerances in terms of smaller feature sizes.

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