RESUMEN
In an effort to study changes in cerebral blood flow (CBF), intracranial pressure (ICP) and intracranial compliance (ICC) simultaneously, we have developed a chronic model in rats using a pial window crown with two ports. This model can also be used to study vasoreactivity of pial vessels. Female Sprague-Dawley rats weighing between 225-250 g underwent placement of cranial chamber with dual ports under pentobarbital anesthesia. To test the utility of this technique 45 groups of rats were studied. Group 1 consisted of control animals. Group 2 consisted of rats undergoing 15 min of global cerebral ischemia. Rats in group 3 were evaluated for changes in vessel diameter and ICP after adenosine injection. In group 4 leukocyte/endothelial interactions were evaluated. These groups demonstrate the ability of this model to monitor CBF, ICP, ICC and pial vessel architecture in chronic rat experiments.