RESUMEN
Lactococcus garvieae is a Gram-positive, catalase negative coccus arranged in pairs or short chains, well-known as a fish pathogen. We report a case of Infective Endocarditis (IE) by L. garvieae in a native valve from a 68-year-old male with unknown history of contact with raw fish and an extensive history of heart disease. This case highlights the reliability of MALDI-TOF MS compared to conventional methods in the identification of rare microorganisms like this.
Asunto(s)
Emigrantes e Inmigrantes , Malaria/diagnóstico , Viaje , Adolescente , Adulto , Anciano , Animales , Niño , Preescolar , Femenino , Hospitales , Humanos , Malaria/terapia , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
MALDI-TOF MS has been evaluated to detect carbapenemases activity and pathogen identification directly from positive blood cultures. 21 non-carbapenemase producers and 19 carbapenemase producers Enterobacteriaceae and Pseudomonas aeruginosa strains were included in the study. This technique is simple and detects carbapenemases in 4.5h with high sensitivity and specificity.
Asunto(s)
Bacteriemia/diagnóstico , Proteínas Bacterianas/análisis , Técnicas Bacteriológicas/métodos , Sangre/microbiología , Enterobacteriaceae/enzimología , Pseudomonas aeruginosa/enzimología , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , beta-Lactamasas/análisis , Bacteriemia/microbiología , Enterobacteriaceae/aislamiento & purificación , Humanos , Pseudomonas aeruginosa/aislamiento & purificación , Factores de TiempoRESUMEN
UNLABELLED: We have evaluated a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method for the identification of carbapenemases and for distinguishing metallo-ß-lactamases (MBLs). A total of 49 noncarbapenemase-producing and 14 carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa clinical strains, previously characterized by PCR, were included in the study. With MALDI-TOF MS, the presence of carbapenemases was confirmed by the detection of ertapenem hydrolysis (lost of molecular peaks: 476·5 Da, 498·5 Da, 520·5 Da and presence of degradation products) in the mixture of the bacteria with the antibiotic, and classification was achieved by selective inhibition of carbapenemase activity (the ertapenem molecular peak was maintained) with ethylenediaminetetraacetic acid (EDTA). We obtained a good concordance among the results of PCR and MALDI-TOF MS. This method appears to be simple, fast and reliable for distinguishing in few hours different classes of carbapenemases, which can be very useful for epidemiological studies or to establish a specific antimicrobial therapy. SIGNIFICANCE AND IMPACT OF THE STUDY: MALDI-TOF mass spectrometry is increasingly present in microbiology laboratories due to its increasing use for bacterial identification. This study describes a method for detection of carbapenemase activity using MALDI-TOF, which is similar to the reference method: the detection of imipenem hydrolysis using UV spectrometry.
Asunto(s)
Proteínas Bacterianas/análisis , Enterobacteriaceae/enzimología , Pseudomonas aeruginosa/enzimología , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , beta-Lactamasas/análisis , Antibacterianos/metabolismo , Proteínas Bacterianas/metabolismo , Ertapenem , Humanos , beta-Lactamasas/metabolismo , beta-Lactamas/metabolismoRESUMEN
A prospective study in 11 consecutive children with gastroduodenal disease is presented. Endoscopic study and gastric biopsies were performed for histologic and bacteriologic research of H. pylori. Endoscopic studies in 7 of the patients showed lesions of antritis that were assessed by histopathologic study. H. pylori was isolated in 4 of them. The presence of H. pylori in the stomach of children with chronic gastritis is confirmed. It is remarked the importance of gastric biopsy, at least when the endoscopic study shows the presence of antral gastritis.