RESUMEN
Nicotine transfer via breast milk induces obesity in the adult offspring. We hypothesize that sympathetic nervous system (SNS) activity, brown adipose tissue (BAT) thermogenesis and white adipose tissue (WAT) lipogenesis/adipogenesis are altered in adult rats that were exposed to nicotine exclusively during the breastfeeding period. Lactating Wistar rats were separated into two groups: nicotine (NIC), dams implanted with osmotic minipumps containing 6 mg/kg of nicotine at postnatal day (PN) 2; control, dams were implanted with saline-containing minipumps. Euthanasia occurred at PN120 or PN180. NIC offspring had lower BAT SNS activity and higher BAT lipid content. NIC males showed lower UCP1, ß3-AR and CPT1a, while NIC females showed lower UCP1, TRα1, CPT1a, suggesting lower thermogenesis. NIC males showed higher WAT SNS activity, WAT ß3-AR, adrenal catecholamine, FAS, PPARγ and adipocytes area, while NIC females showed higher ACC, FAS, CEBPß and PPARγ. These findings indicate increased lipogenesis/adipogenesis in both sexes, with a possible compensatory sympathetic activated-lipolysis in males. NIC males had higher hypothalamic pAMPK/AMPK, explaining the lower BAT sympathetic activity. Neonatal nicotine exposure reduces BAT SNS activity and thermogenesis, and, only in males, increases WAT adipogenesis/lipogenesis, despite higher WAT SNS activity. These alterations can be associated with obesogenesis in this programming model.
Asunto(s)
Tejido Adiposo Pardo/efectos de los fármacos , Tejido Adiposo Blanco/efectos de los fármacos , Lactancia , Nicotina/toxicidad , Factores Sexuales , Tejido Adiposo Pardo/fisiología , Tejido Adiposo Blanco/fisiología , Animales , Femenino , Lipogénesis , Masculino , Ratas , Ratas Wistar , TermogénesisRESUMEN
AIMS: Maternal smoking is considered a risk factor for childhood obesity. In a rat model of tobacco exposure during breastfeeding, we previously reported hyperphagia, overweight, increased visceral fat and hyperleptinemia in adult female offspring. Obesity and eating disorders are associated with impairment in the endocannabinoid (EC) and dopaminergic (DA) systems. Considering that women are prone to eating disorders, we hypothesize that adult female Wistar rats that were exposed to cigarette smoke (CS) during the suckling period would develop EC and DA systems deregulation, possibly explaining the eating disorder in this model. MATERIAL AND METHODS: To mimic maternal smoking, from postnatal day 3 to 21, dams and offspring were exposed to a smoking machine, 4×/day/1â¯h (CS group). Control animals were exposed to ambient air. Offspring were evaluated at 26â¯weeks of age. KEY FINDINGS: Concerning the EC system, the CS group had increased expression of diacylglycerol lipase (DAGL) in the lateral hypothalamus (LH) and decreased in the liver. In the visceral adipose tissue, the EC receptor (CB1r) was decreased. Regarding the DA system, the CS group showed higher dopamine transporter (DAT) protein expression in the prefrontal cortex (PFC) and lower DA receptor (D2r) in the arcuate nucleus (ARC). We also assessed the hypothalamic leptin signaling, which was shown to be unchanged. CS offspring showed decreased plasma 17ß-estradiol. SIGNIFICANCE: Neonatal CS exposure induces changes in some biomarkers of the EC and DA systems, which can partially explain the hyperphagia observed in female rats.
Asunto(s)
Neuronas Dopaminérgicas/efectos de los fármacos , Endocannabinoides/metabolismo , Contaminación por Humo de Tabaco/efectos adversos , Animales , Animales Recién Nacidos , Fumar Cigarrillos , Dopamina/metabolismo , Proteínas de Transporte de Dopamina a través de la Membrana Plasmática/efectos de los fármacos , Neuronas Dopaminérgicas/fisiología , Endocannabinoides/fisiología , Femenino , Área Hipotalámica Lateral/efectos de los fármacos , Área Hipotalámica Lateral/metabolismo , Hipotálamo/metabolismo , Lactancia/efectos de los fármacos , Leptina/metabolismo , Lipoproteína Lipasa/efectos de los fármacos , Exposición Materna/efectos adversos , Obesidad/etiología , Obesidad/metabolismo , Ratas , Ratas Wistar , Receptores de Cannabinoides/efectos de los fármacos , Fumar , NicotianaRESUMEN
We study general multifractal properties of tidal gauge and long-wave time series which show a well defined transition between two states, as is the case of sea level when a tsunami arrives. We adopt a method based on discrete wavelets, called wavelet leaders, which has been successfully used in a wide range of applications from image analysis to biomedical signals. First, we analyze an empirical time series of tidal gauge from the tsunami event of 27 February 2010 in Chile. Then, we study a numerical solution of the driven-damped regularized long-wave equation (RLWE) which displays on-off intermittency. Both time series are characterized by a sudden change between two sharply distinct dynamical states. Our analysis suggests a correspondence between the pre- and post-tsunami states (ocean background) and the on state in the RLWE, and also between the tsunami state (disturbed ocean) and the off state in the RLWE. A qualitative similarity in their singularity spectra is observed, and since the RLWE is used to model shallow water dynamics, this result could imply an underlying dynamical similarity.
Asunto(s)
Terremotos/estadística & datos numéricos , Fractales , Modelos Estadísticos , Dinámicas no Lineales , Tsunamis/estadística & datos numéricos , Análisis de Ondículas , Chile , Simulación por ComputadorRESUMEN
The aim of this study was determine whether the introduction of a high-fat diet during the peripubertal phase induces significant changes in body weight control, glucose homeostasis and the parasympathetic tonus compared with the administration of this diet to adult rats. High-fat diet was offered to male Wistar rats at weaning or during adulthood. A group of rats received high-fat diet for 60 days, from weaning to 81-day-old (HF81) or from 60 to 120-day-old (HF120), whereas 2 other groups received a normal-fat diet (i. e., NF81 and NF120). We analyzed adiposity, glucose homeostasis, insulin sensitivity, and vagal nerve activity. High-fat diet increased the accumulation of adipose tissue in all of the rats, but the difference was greater in the rats that were fed the high-fat diet since weaning (p<0.001). The HF rats showed glucose intolerance with high levels of insulin secretion during the glucose tolerance test (p<0.01). Rats that were fed the high-fat diet presented severe insulin resistance, indicated by a low K itt (p<0.01). Interestingly, the HF81 rats exhibited greater insulin resistance compared with the HF120 rats (p<0.05). The recordings of vagus nerve activity showed that the HF rats had higher parasympathetic activity than the NF rats irrespective of age (p<0.01). Our results show that a high-fat diet offered to rats just after weaning or in adulthood both cause impairment of glycemic homeostasis and imbalance in parasympathetic activity. Importantly, the consumption of high-fat diet immediately after weaning has more drastic consequences compared with the consumption of the same diet during adulthood.
Asunto(s)
Envejecimiento/metabolismo , Dieta Alta en Grasa/efectos adversos , Tejido Adiposo/efectos de los fármacos , Envejecimiento/efectos de los fármacos , Animales , Glucemia/efectos de los fármacos , Glucemia/metabolismo , Fenómenos Electrofisiológicos/efectos de los fármacos , Conducta Alimentaria/efectos de los fármacos , Prueba de Tolerancia a la Glucosa , Homeostasis/efectos de los fármacos , Insulina/sangre , Insulina/farmacología , Masculino , Ratas , Ratas Wistar , Nervio Vago/efectos de los fármacos , Nervio Vago/fisiologíaRESUMEN
The link between phase coherence and non-Gaussian statistics is investigated using magnetic field data observed in the solar wind turbulence near the Earth's bow shock. The phase coherence index Cphi, which characterizes the degree of phase correlation (i.e., nonlinear wave-wave interactions) among scales, displays a behavior similar to kurtosis and reflects a departure from Gaussianity in the probability density functions of magnetic field fluctuations. This demonstrates that nonlinear interactions among scales are the origin of intermittency in the magnetic field turbulence.
RESUMEN
To determine the occurrence and epidemiological aspects of intestinal parasites among the Parakanã indigenous people in the Paranatinga settlement (in the eastern Amazon Region), parasitological tests were performed in April 1992 and February 1995. One fresh stool specimen was obtained and immediately processed using the Hoffman and direct methods. Some 126 samples were obtained in April 1992 (from a total population of 215 individuals). Some 80. 2% (101) of those tested were infected with at least one species of intestinal parasite. Hookworms were found in 33.3%, Ascaris lumbricoides 42.8%, Trichuris trichiura 0.8%, and Strongyloides stercoralis 5.6%. Entamoeba histolytica and Giardia lamblia protozoans were found in 65.0% and 46.8% of those tested, respectively. A second parasitological survey was performed on 174 individuals (from a population of 253) in February 1995. 88.5% were infected. Note that prevalence in February 1995 was higher than in April 1992 (p=0.04). It was lower for hookworms, E. histolytica, and G. lamblia, with no S. stercoralis (p<0.05). Despite provision of health care in the Paranatinga community, prevalence of intestinal parasites is still extremely high, suggesting that primary and secondary health care should be increased immediately to increase the efficacy of prevention of intestinal parasites.