RESUMEN
OBJECTIVE: Here we determine the salivary levels of leukotriene B4 (LTB4 ) and its relation with salivary mucin and alveolar bone level. BACKGROUND: LTB4 is a membrane-derived lipid mediator formed from arachidonic acid. It is among the most potent stimulants of polymorphonuclear leukocytes providing the first host defense against infections. Leukotrienes also induce bone resorption. Because LTB4 is present in the oral cavity the aim of the present study was to explore the role of LTB4 in patients with periodontal disease. METHODS: Eighty-one subjects were clinically examined and distributed into four groups, namely, clinically healthy, mild, moderate and severe periodontitis, according to periodontal status, classified into values of clinical attachment level and probing pocket depth. Unstimulated saliva was collected for 5 min. Salivary LTB4 was determined by an immune assay method, mucin was determined by a colorimetric method and radiographic assessment used to determine alveolar bone level. RESULTS: Patients with mild periodontitis showed a decrease in salivary LTB4 levels while patients with severe periodontitis showed increased LTB4 levels. A significant positive correlation was observed between salivary LTB4 and clinical attachment level, salivary mucin concentration or alveolar bone level. CONCLUSION: The close relation between salivary LTB4 and mucin levels suggested that LTB4 might be involved in the defense mechanism of the oral cavity. The correlation of LTB4 with the alveolar bone level indicates that they are one of the mediators responsible for bone resorption.
Asunto(s)
Pérdida de Hueso Alveolar/clasificación , Leucotrieno B4/análisis , Mucinas/análisis , Periodontitis/clasificación , Saliva/química , Proteínas y Péptidos Salivales/análisis , Adulto , Pérdida de Hueso Alveolar/diagnóstico por imagen , Pérdida de Hueso Alveolar/inmunología , Proceso Alveolar/diagnóstico por imagen , Colorimetría/métodos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neutrófilos/inmunología , Pérdida de la Inserción Periodontal/clasificación , Pérdida de la Inserción Periodontal/inmunología , Índice Periodontal , Bolsa Periodontal/clasificación , Bolsa Periodontal/inmunología , Periodontitis/inmunología , Periodoncio/inmunología , Periodoncio/metabolismo , Radiografía de Mordida Lateral/métodos , Saliva/inmunologíaRESUMEN
OBJECTIVE: Here we determine the relationship between salivary levels of mucin and amylase and the clinical parameters of periodontal disease before and after periodontal treatment. SUBJECTS: Ninety two subjects were clinically examined and distributed into four groups namely clinically healthy, mild, moderate and severe periodontitis, according to the periodontal status, classified according the values of clinical attachment level (CAL) and probing pocket depth (PPD). Unstimulated saliva was collected for 5 min. Salivary proteins, amylase and mucin were determined by colorimetric methods. RESULTS: A significant positive correlation (P < 0.0001) was observed between salivary mucin, amylase or protein and PPD or CAL before periodontal treatment while flow rate showed a negative correlation. Mucin and amylase output also showed a positive correlation with PPD or CAL. After treatment, the improvement of clinical parameters was accompanied by a diminution of salivary mucin, amylase or protein concentration and output in moderate and severe group. CONCLUSIONS: The increment of mucin and amylase output in relation to periodontal status indicates that salivary glands respond to the disease by increasing the protective potential of saliva when necessary and return to the normal rate of secretion after the resolution of the inflammatory process.