Asunto(s)
Actinas/fisiología , Citoesqueleto/fisiología , Genes ras , Oncogenes , Proteínas Serina-Treonina Quinasas/fisiología , Actinas/ultraestructura , Animales , Tamaño de la Célula/fisiología , Transformación Celular Neoplásica , Fibroblastos/citología , Fibroblastos/fisiología , Péptidos y Proteínas de Señalización Intracelular , Ratones , Proteínas Serina-Treonina Quinasas/genética , Transfección , Quinasas Asociadas a rhoRESUMEN
The conventional experimental techniques showed that ajmaline derived from Rouwolfia serpentina Benth. biomass grown in tissue culture was effective in arresting canine ventricular arrhythmias induced by coronary occlusion, strophanthin and epinephrine, displaying antiarrhythmic effects in aconitine-induced intoxication of rats and substantially elevating the threshold of ventricular fibrillations in cats. The antiarrhythmic activity of ajmaline produced by the biotechnological technique was identical to that of ajmaline derived from the natural root of Rauwolfia serpentina Benth, which allows one to recommend that the agent in question should be clinically tested as an antiarrhythmic.
Asunto(s)
Ajmalina/farmacología , Arritmias Cardíacas/tratamiento farmacológico , Plantas Medicinales , Rauwolfia , Ajmalina/aislamiento & purificación , Animales , Gatos , Técnicas de Cultivo , Perros , Ratones , Ratas , Ratas EndogámicasRESUMEN
The construction and application of a fractional factorial design of the type N = 2(5-2) for the preparation of effervescent acetylsalicylic acid tablets by a new technique was demonstrated. This method involved direct compression of the tablet ingredients, dipping the tablets in a volatile mixed solvent system and subsequent removal of the solvent. With the help of a derived regression equation, the factors considered most likely to have a beneficial effect on tablet hardness were: the compression force, the percent of acetylsalicylic acid content/tablet, the particle size of tablet ingredients and the solvent ratio. The time of dipping was found to have an insignificant effect on tablet hardness. By following the path of the steepest ascent, effervescent acetylsalicylic acid tablets of remarkable hardness, friability and dissolution time were obtained. The optimum conditions for preparing these tablets were determined.