RESUMEN
Two new limonoids, 12-ethoxynimbolinins G and H (compounds 1 and 2), and one known compound, toosendanin (Chuanliansu) (compound 3), were isolated from the bark of Melia toosendan. Their structures were elucidated by spectroscopic analysis and X-ray techniques. The absolute configuration of toosendanin (3) was established by single-crystal X-ray diffraction. Compounds 1-3 were evaluated for their cytotoxicity against five tumor cell lines.
Asunto(s)
Limoninas/aislamiento & purificación , Melia/química , Corteza de la Planta/química , Extractos Vegetales/química , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Humanos , Estructura Molecular , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Difracción de Rayos XRESUMEN
In the present study, two new limonoids, 1α, 7α-dihydroxyl-3α-acetoxyl-12α-ethoxylnimbolinin (1) and 1α-tigloyloxy-3α-acetoxyl-7α-hydroxyl-12ß-ethoxylnimbolinin (2), together with other four known limonoids (3-6), were isolated from the fruits of Melia toosendan. Their structures were elucidated by means of extensive spectroscopic analyses (NMR and ESI-MS) and comparisons with the data reported in the literature. The isolated compounds were evaluated for their antibacterial activities. Compound 4 exhibited significant antibacterial activity against an oral pathogen, Porphyromonas gingivalis ATCC 33277, with an MIC value of 15.2 µg·mL(-1). Compound 2 was also active against P. gingivalis ATCC 33277, with an MIC value of 31.25 µg·mL(-1). In conlcusion, our resutls indicate that these compounds may provide a basis for future development of novel antibiotics.
Asunto(s)
Antibacterianos/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Limoninas/aislamiento & purificación , Melia/química , Antibacterianos/química , Antibacterianos/farmacología , Medicamentos Herbarios Chinos/química , Frutas/química , Limoninas/química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Porphyromonas gingivalis/efectos de los fármacos , Porphyromonas gingivalis/crecimiento & desarrollo , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
The present study was designed to isolate and evaluate the antibacterial activity of the compounds from the whole plant of Euphorbia helioscopia L.. Various chromatographic techniques were used to isolate and purify the compound. The structure of the compound was elucidated on basis of spectral data ((1)H NMR, (13)C NMR, (1)H-(1)H COSY, HSQC, HMBC, NOESY, IR, and HR-ESI-MS). A new jatrophone-type diterpenoid (14α,15ß-diacetoxy-3ß-benzoyloxy-7ß-nicotinoyloxy-9-oxo-jatropha-5E,11E-diene), named euphoheliosnoid E (1), was isolated from the whole plant of E. helioscopia L. Compound 1 showed significant anti-microbial activity against oral pathogens.
Asunto(s)
Diterpenos/aislamiento & purificación , Euphorbia/química , Niacina/análogos & derivados , Extractos Vegetales/química , Antiinfecciosos/aislamiento & purificación , Antiinfecciosos/farmacología , Diterpenos/química , Diterpenos/farmacología , Estructura Molecular , Enfermedades de la Boca/microbiología , Niacina/química , Niacina/aislamiento & purificación , Niacina/farmacología , Extractos Vegetales/farmacologíaRESUMEN
Four new limonoids (1-4), together with five known limonoids (5-9), were isolated from the fruits of Melia toosendan. Their structures were elucidated based on extensive spectroscopic analyses (1D- and 2D-NMR, HRESIMS, IR, [α](D)). The isolated compounds were evaluated for their neurite outgrowth-promoting activities. Compounds 2 and 6 significantly enhanced NGF-mediated neurite outgrowth in PC12 cells at concentrations ranging from 0.1 to 50.0 µM.
Asunto(s)
Limoninas/farmacología , Melia/química , Factor de Crecimiento Nervioso/metabolismo , Extractos Vegetales/farmacología , Animales , Frutas , Limoninas/química , Limoninas/aislamiento & purificación , Estructura Molecular , Neuritas/efectos de los fármacos , Células PC12 , Extractos Vegetales/química , RatasRESUMEN
To study the chemical constituents of the fruits of Melia toosendan, three limonoids were isolated and purified by repeated silica gel column chromatography and preparative HPLC from the EtOAc extract of M. toosendan. Their structures were determined by their physico-chemical properties and spectroscopic data (1D-NMR, 2D-NMR) as: 24, 25, 26, 27-tetranorapotirucalla-(apoeupha)-1alpha-tigloyloxy-3alpha, 7alpha-dihydroxyl-12alpha-acetoxyl-14, 20, 22-trien-21, 23-epoxy-6, 28-epoxy (1), nimbolinin B (2), and trichilinin D (3), separately. Compound 1 is a new compound, and compound 2 is obtained from this plant for the first time.
Asunto(s)
Limoninas/aislamiento & purificación , Melia/química , Plantas Medicinales/química , Frutas/química , Limoninas/química , Estructura MolecularRESUMEN
Toosendanone A (1), a new euphane (tirucallane)-type triterpene bearing a five-membered ring in the side chain and the first cyclopentanyl protolimonoid, was isolated from the bark of Melia toosendan, along with two new tirucallanes, toosendanic acids A (2) and B (3). The structure and absolute configuration of compound 1 was elucidated by spectroscopic data interpretation and X-ray diffraction analysis. Compounds 1-3 were evaluated for cytotoxicity against a small panel of cancer cell lines.
Asunto(s)
Antineoplásicos Fitogénicos/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Melia/química , Triterpenos/aislamiento & purificación , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Células HL-60 , Células HT29 , Humanos , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Triterpenos/química , Triterpenos/farmacologíaRESUMEN
OBJECTIVE: To study the constituents of the whole herbs of Vernonia cinerea. by bio-activity guided isolation with PC-12 model. METHOD: The constituents were separated by column chromatography and the structures were elucidated by spectroscopic methods. RESULT: Four compounds were identified to be (+)-lirioresinol B (1), stigmasterol (2), stigmasterol-3-O-beta-D-glucoside (3), 4-sulfo-benzocyclobutene (4), and their NGF inducing activity were also investigated. CONCLUSION: Compounds 1, 3, 4 were isolated from this genus for the first time, and compound 4 was identified as a new natural product. Compounds 1, 3, 4 showed cytotoxicity on PC-12, and compounds 2, 3, 4 showed inhibition activity. Compound 4 showed a specific effect on the survival of TrkA fibroblasts, and resulted in the inducing NGF activity.
Asunto(s)
Medicamentos Herbarios Chinos/química , Vernonia/química , Animales , Glucósidos/química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Células PC12 , Compuestos Policíclicos/química , Compuestos Policíclicos/farmacología , Ratas , Estigmasterol/análogos & derivados , Estigmasterol/químicaRESUMEN
A new elemanolide sesquiterpene lactone, named elescaberin (1), together with two known compounds, namely, isodeoxyelephantopin (2) and deoxyelephantopin (3), was isolated from the whole plant of Elephantopus scaber. The structure of 1 was elucidated on the basis of spectroscopic analysis. All three compounds exhibited significant inhibitory activities against human SMMC-7721 liver cancer cells in vitro (IC(50) 8.18-14.08 micromol/l).
Asunto(s)
Antineoplásicos Fitogénicos/aislamiento & purificación , Asteraceae/química , Medicamentos Herbarios Chinos/química , Lactonas/aislamiento & purificación , Sesquiterpenos/aislamiento & purificación , Antineoplásicos Fitogénicos/química , Línea Celular Tumoral , Humanos , Lactonas/química , Estructura Molecular , Sesquiterpenos/química , Sesquiterpenos de Germacrano/aislamiento & purificaciónRESUMEN
In this study five new limonoids, toosendone [24,25,26,27-tetra-nor-6alpha-acetoxy-21,22-epoxy-7alpha-tigloyl-1alpha,3alpha,28-trihydroxyapotirucalla-(apoeupha)-14,20,22-trien-12-one, 1] and 12-ethoxynimbolinins A-D (2-5), together with five known limonoids, 1-acetyltrichilinin (6), 1-cinnamoyltrichilinin (7), trichilinin B (8), 1,7-di-O-acetyl-14,15-deoxyhavanensin (9) and 12-O-methylnimbolinin B (10),were isolated from the fruits of Melia toosendan. Their structures and relative configurations were established based on spectroscopic analysis. Compound 4 exhibited significant antibacterial activity against the oral pathogen, Porphyromonas gingivalis ATCC 33 277, with an MIC value of 15.6 microg/mL. Compounds 7 and 8 were also active against P. gingivalis ATCC 33 277, with MIC values of 31.3 and 31.5 microg/mL respectively.
Asunto(s)
Antibacterianos/farmacología , Limoninas/farmacología , Melia/química , Porphyromonas gingivalis/efectos de los fármacos , Streptococcus mutans/efectos de los fármacos , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Frutas/química , Humanos , Limoninas/química , Limoninas/aislamiento & purificación , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Boca/microbiología , Plantas Medicinales/químicaRESUMEN
There were five new diterpenoids, 18-beta-D-3',4'-diacetoxyxylopyranosyl-ent-kaur-16-ene (1), 18-beta-L-3',5'-diacetoxyarabinofuranosyl-ent-kaur-16-ene (2), 18-beta-D-3',6'-diacetoxyglucopyranosyl-ent-kaur-16-ene (3), ent-isopimar-8(14),15-dien-19-oic acid (4), and 5alpha-hydroxy-ent-rosa-15-en-18-oic acid (5), isolated from the whole herb of Sagittaria pygmaea. Their structures and relative configurations were established based on spectroscopic studies, chemical methods, and X-ray crystallographic analysis. Compound 2 exhibited significant antibacterial activity against the oral pathogens, Streptococcus mutans ATCC 25 175 and Actinomyces viscosus ATCC 27 044, with MIC values against both pathogens of 15.6 microg/mL. Compound 3 was active against only A. viscosus ATCC 27 044 with an MIC value of 62.5 microg/mL. Compounds 4 and 5 were active against S. mutans ATCC 25 175 and A. viscosus ATCC 27 044, with MIC values against both pathogens of 125.0 microg/mL.
Asunto(s)
Aggregatibacter actinomycetemcomitans/efectos de los fármacos , Antibacterianos/farmacología , Bacterias Grampositivas/efectos de los fármacos , Fitoterapia , Extractos Vegetales/farmacología , Sagittaria , Actinomyces viscosus/efectos de los fármacos , Antibacterianos/administración & dosificación , Antibacterianos/química , Antibacterianos/uso terapéutico , Diterpenos/administración & dosificación , Diterpenos/química , Diterpenos/farmacología , Diterpenos/uso terapéutico , Humanos , Pruebas de Sensibilidad Microbiana , Extractos Vegetales/administración & dosificación , Extractos Vegetales/química , Extractos Vegetales/uso terapéutico , Streptococcus mutans/efectos de los fármacosRESUMEN
In order to look for lower toxic and strongly active compounds, the structure of sesquiterpene lactones from Elephantopus scaber was modified. Deoxyelephantopin and scabertopin were isolated from the whole plant of Elephantopus scaber and hydrogenated and epoxidated. Five sesquiterpenoide derivatives were prepared and their structures were identified by IR, NMR and MS spectra analysis. Four sesquiterpenoides are new compounds. Part of the compounds show definite antitumor activity.
Asunto(s)
Antineoplásicos Fitogénicos/química , Asteraceae , Lactonas/química , Sesquiterpenos/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Asteraceae/química , Células HeLa , Humanos , Hidrogenación , Lactonas/aislamiento & purificación , Lactonas/farmacología , Estructura Molecular , Plantas Medicinales/química , Sesquiterpenos/aislamiento & purificación , Sesquiterpenos/farmacología , Terpenos/química , Terpenos/farmacologíaRESUMEN
Seven new ent-rosane diterpenoids, sagittines A-G (1-7), together with one new labdane diterpene, 13-epi-manoyl oxide-19-O-alpha-l-2',5'-diacetoxyarabinofuranoside (8), were isolated from the whole plant of Sagittaria sagittifolia. The structures and relative configurations of 1-8 were characterized using spectroscopic means, chemical methods, and X-ray crystallography. Compounds 1-4 exhibited antibacterial activity against the oral pathogens Streptococcus mutans ATCC 25175 and Actinomyces naeslundiis ATCC 12104, with MIC values between 62.5 and 125 microg/mL. Compound 5 was active against only A. naeslundiis ATCC 12104, with an MIC value of 62.5 microg/mL.
Asunto(s)
Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Arabinosa/análogos & derivados , Diterpenos/aislamiento & purificación , Diterpenos/farmacología , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Plantas Medicinales/química , Sagittaria/química , Actinomyces/efectos de los fármacos , Antibacterianos/química , Arabinosa/química , Arabinosa/aislamiento & purificación , Arabinosa/farmacología , Cristalografía por Rayos X , Diterpenos/química , Medicamentos Herbarios Chinos/química , Pruebas de Sensibilidad Microbiana , Conformación Molecular , Estructura Molecular , Streptococcus mutans/efectos de los fármacosRESUMEN
Three new diterpenoids, yuexiandajisu D (1), E (2) and F were isolated from the roots of Euphorbia ebracteolata, along with eight known diterpenoids, jolkinolide B (4), jolkinolide A, ent-11alpha-hydroxyabieta-8(14),13(15)-dien-16,12alpha-olide (6), ent-(13S)-hydroxyatis-16-ene-3,14-dione, ent-3beta,(13S)-dihydroxyatis-16-en-14-one, ent-3-oxokaurane-16alpha,17-diol, ent-16alpha,17-dihydroxyatisan-3-one and ent-atisane-3beta,16alpha,17-triol. The structures of all compounds were deduced using spectroscopic methods and confirmed for 1 and 2 by single-crystal X-ray diffraction. A biogenetic pathway for the formation of 1 and 2 is proposed briefly. Cytotoxic activities were evaluated against ANA-1, B 16 and Jurkat tumor cells. Jolkinolide B (4) displayed modest activity on ANA-1, B 16 and Jurkat tumor cells with IC50 values 4.46 x 10(-2), 4.48 x 10(-2), 6.47 x 10(-2) microM, and ent-11alpha-hydroxyabieta-8(14),13(15)-dien-16,12alpha-olide (6) showed significant activity against ANA-1 and Jurkat cells with IC50 values 7.12 x 10(-3) and 1.79 x 10(-2) microM. Compound 1 was found to be slightly active against ANA-1 cells with an IC50 value 2.88 x 10(-1)microM. Structure-activity relationships of isolated compounds are also discussed.
Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Euphorbia , Fitoterapia , Extractos Vegetales/farmacología , Animales , Antineoplásicos Fitogénicos/administración & dosificación , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/uso terapéutico , Línea Celular Tumoral , Diterpenos/administración & dosificación , Diterpenos/química , Diterpenos/farmacología , Diterpenos/uso terapéutico , Humanos , Concentración 50 Inhibidora , Ratones , Extractos Vegetales/administración & dosificación , Extractos Vegetales/química , Extractos Vegetales/uso terapéutico , Raíces de Plantas , Relación Estructura-Actividad , Difracción de Rayos XRESUMEN
A new compound, 3'(R)-O-beta-D-glucopyranosyl-3',4'-dihydroxanthyletin (1), and a known compound, prim-O-glucosylcimifugin (2), were isolated from the roots of Peucedanum dissolutum. The structure of 1 was elucidated by spectral evidence and chemical reaction. The NMR signals of carbons and protons of 2 were assigned for the first time by analysis of (1)H-(1)H COSY, HMQC and HMBC spectra.
Asunto(s)
Apiaceae/química , Monosacáridos/química , Xantenos/química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Monosacáridos/aislamiento & purificación , Raíces de Plantas/química , Xantenos/aislamiento & purificaciónRESUMEN
Three new macrocyclic diterpenes, kansuinins F (1), G (2), and H (3), together with four known jatrophane diterpenes, kansuinins D (4), E (5), and A (6) and 3beta,5alpha,7beta,15beta-tetraacetoxy-9alpha-nicotinoyloxyjatropha-6(17)-11E-dien-14-one, were isolated from the roots of Euphorbia kansui. Compounds 1 and 2 were assigned as 6(17)-en-11,12-epoxy-14-one-type jatrophane diterpenes, and compound 3 as a 6(17)-en-11,14-epoxy-12-one jatrophane diterpene. The structures of compounds 1-3 and the relative configurations of compounds 4 and 5 were determined by spectral data analysis. Kansuinin E (5) exhibited a specific survival effect on fibroblasts that expressed TrkA, a high-affinity receptor for nerve growth factor.
Asunto(s)
Diterpenos/aislamiento & purificación , Euphorbia/química , Plantas Medicinales/química , Diterpenos/química , Diterpenos/farmacología , Fibroblastos/metabolismo , Medicina Tradicional China , Estructura Molecular , Factor de Crecimiento Nervioso/efectos de los fármacos , Raíces de Plantas/química , Receptor trkA/efectos de los fármacosRESUMEN
With a history of several thousand years, traditional Chinese medicine has been well documented to be effective in the treatment of various disorders. We have investigated the activities of potential neuroactive compounds in traditional Chinese medicine such as Melia toosendan using an in vitro model system, rat pheochromocytoma PC12 cells. We report here that treatment of PC12 cells with a crude extract of the fruits of M. toosendan reduces cell growth in a dose-dependent manner without detectable cytotoxicity. Upon treatment with M. toosendan, PC12 cells exhibit robust neurite outgrowth, to a greater extent than that observed with nerve growth factor. Results obtained with specific kinase inhibitors and protein kinase A-deficient PC12 cells indicate that the actions of M. toosendan are mediated by the activation of protein kinase A and extracellular signal-regulated kinases.
Asunto(s)
Diferenciación Celular/efectos de los fármacos , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Medicamentos Herbarios Chinos/farmacología , Melia/química , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Animales , Western Blotting/métodos , Células COS/efectos de los fármacos , Diferenciación Celular/fisiología , División Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Chlorocebus aethiops , Medio de Cultivo Libre de Suero/farmacología , Relación Dosis-Respuesta a Droga , Interacciones Farmacológicas , Activación Enzimática/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , L-Lactato Deshidrogenasa , Factor de Crecimiento Nervioso/farmacología , Neuritas/efectos de los fármacos , Células PC12 , Ratas , Receptor trkA/metabolismo , Sales de Tetrazolio , Tiazoles , Factores de TiempoRESUMEN
AIM: In order to compare the calcium antagonist activity between the derivatives of (+)-praeruptorin A with C-3' and C-4' cis-configuration and trans-configuration, and to look for new active compounds, some derivatives with C-3', C-4' trans-configuration of (+)-praeruptorin A were semi-synthsized. METHODS: (+)-Praeruptorin A was isolated from the root of Peucedanum praeruptorum. Basic hydrolysis of (+)-praeruptorin A was carried out. From the alkaline hydrolysis product (2), eight new products (5-12) with C-3', C-4' trans-configuration were semi-synthsized whose C-3' was linked to angeloyloxy and C-4' was linked to various acyloxy, using respective acids as acylating agents, DCC as a dehydrant, DMAP as catalyst. From the alkaline hydrolysis product (4), five new products (13-17) with C-3', C-4' trans-configuration were obtained whose C-3', C-4' is linked to various same acyloxys, using respective acids as acylating agents, DCC as dehydrant, DMAP as catalyst. Also from the alkaline hydrolysis product (4), using respective acyl chlorides as acylating agents, anhydrous dichloromethane containing minor pyridine as a solvent, the improved Schotten-Baumann reactions were carried out, two new products (18, 20) with C-3', C-4' trans-configuration were obtained whose only C-3' linked to acyloxy and two other new products (19, 21) with C-3', C-4' trans-configuration were obtained whose C-3' and C-4' linked two acyloxys. The structures of all the products were elucidated by spectral analyses including IR, 1HNMR and EIMS. The calcium antagonist activity of all of the products were tested by inhibition of the systole of rat artery ring. RESULTS: Seventeen compounds with C-3', C-4' trans-configuration were semi-synthesized from (+)-praeruptorin A for the first time and their calcium antagonist activity were evaluated. CONCLUSION: All of the derivatives were new compounds. Bioactivity assay indicated that some new compounds with C-3', C-4' trans-configuration showed obvious calcium antagonist activity, but they are not as strong as (+)-praeruptorin A. The activity of some products was shown to be similar to that of the derivatives with C-3', C-4' cis-configuration for the first time.
Asunto(s)
Cumarinas/síntesis química , Medicamentos Herbarios Chinos/síntesis química , Animales , Aorta Torácica/efectos de los fármacos , Bloqueadores de los Canales de Calcio/síntesis química , Bloqueadores de los Canales de Calcio/farmacología , Cumarinas/química , Cumarinas/farmacología , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Estructura Molecular , Contracción Muscular/efectos de los fármacos , Músculo Liso Vascular/efectos de los fármacos , Ratas , EstereoisomerismoRESUMEN
Two new triterpenoid saponins, 3beta-O-beta-D-xylopyranosyl (1 --> 3)-alpha-L-rhamnopyranosyl-pyrocincholic acid 28-O-beta-D-glucopyranosyl (1 --> 6)-beta-D-glucopyranosyl ester (1), and 3beta-O-beta-D-xylopyranosyl(l --> 3)-alpha-L-rhamnopyranosyl-cincholic acid 28-O-beta-D-glucopyranosyl ester (2) were isolated from the roots of Adina pilulifera, together with 18 known compounds. The structures of 1 and 2 were determined by spectroscopic methods.
Asunto(s)
Cinchona , Fitoterapia , Extractos Vegetales/química , Saponinas/química , Triterpenos/química , Humanos , Hidrólisis , Espectroscopía de Resonancia Magnética , Raíces de PlantasRESUMEN
OBJECTIVE: To isolate and elucidate the chemical constituents of the whole plant of Vernonia patula, and to provide samples for biological activity screening. METHOD: The chromatography on silica gel was used and the structures were determined by IR, NMR and MS spectra analysis and physicochemical property comparion. RESULT: Four triterpenoids were isolated and identified as bauerenyl acetate(I), friedelin(II), epifriedelanol(III), 20(30)-taraxastene-3 beta, 21 alpha-diol(IV). CONCLUSION: Compounds I and IV were isolated from genus Vernonia for the first time and compounds II and III were isolated from the whole plant of V. patula for the first time. The four compounds show no inhibitory effect on the growing of PC-12 cells.
Asunto(s)
Medicamentos Herbarios Chinos/farmacología , Ácido Oleanólico/análogos & derivados , Plantas Medicinales/química , Triterpenos/aislamiento & purificación , Vernonia/química , Animales , División Celular/efectos de los fármacos , Medicamentos Herbarios Chinos/aislamiento & purificación , Ácido Oleanólico/química , Ácido Oleanólico/aislamiento & purificación , Ácido Oleanólico/farmacología , Células PC12 , Ratas , Triterpenos/química , Triterpenos/farmacologíaRESUMEN
AIM: To study the chemical constituents of the stems of Opuntia vulgaris Mill(Cactaceae). METHODS: The compounds of Opuntia vulgaris were isolated by chromatography of Amberlite Dowex 50 and silica gel, and identified by means of UV, IR, MS, 1D and 2D NMR. RESULTS: Three compounds were isolated and identified as: opuntin B(I), 4-hydroxyproline(II) and tyrosine(III). CONCLUSION: Compound I is a new alkaloid.