RESUMEN
Bioactive glass is an attractive biomaterial that has shown excellent osteogenic and angiogenic effects for oral bone repairing procedures. However, anti-biofilm potential related to such biomaterial has not been completely validated, mainly against multi-species biofilms involved in early tissue infections. The aim of the present study was to evaluate the anti-biofilm effect of 58 S bioactive glass embedding calcium bromide compounds at different concentrations. Bioactive glass free or containing 5, or 10 wt % CaBr2 was synthesized by alkali sol-gel method and then characterized by physco-chemical analyses and scanning electron microscopy (SEM). Then, samples were tested by microbiological assays using optical density, real time q-PCR, and SEM. Bioactive glass particles showed accurate chemical composition and an angular shape with a bimodal size distribution ranging from 0.6 to 110 µm. The mean particle size was around 29 µm. Anti-biofilm effect was recorded for 5 wt % CaBr2 -doped bioactive glass against S. mitis, V. parvula, P. gingivais, S. gordoni, A. viscosus, F, nucleatum, P. gingivais. F. nucleatum, and P. gingivalis. Such species are involved in the biofilm structure related to infections on hard and soft tissues in the oral cavity. The incorporation of calcium bromide into bioactive glass can be a strategy to enhance the anti-biofilm potential of bioactive glasses for bone healing and infection treatment. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1994-2003, 2017.
Asunto(s)
Bacterias/crecimiento & desarrollo , Fenómenos Fisiológicos Bacterianos , Biopelículas/crecimiento & desarrollo , Bromuros/química , Vidrio/química , Boca/microbiologíaRESUMEN
Bioactive glasses (BGs) are promising materials for bone repair due to their desirable properties such as osteoconductivity, biodegradability, angiogenic potential, and antibacterial activity. Ionic dissolution products from bioactive glasses increase the medium pH inhibiting surrounding bacteria proliferation. The activity of BGs against biofilm formation has been enhanced by incorporating organic antibacterial compounds. The aim of this review was to summarize evidence in literature which assesses the efficacy of antibacterial and anti-biofilm compounds embedded in bioactive glasses to prevent peri-implant infection during bone healing. A PubMed bibliographical research was carried out including articles published in the last 20 years. Most previous studies evaluated antibacterial efficiency in planktonic cultures but did not investigate biofilm inhibition, underestimating biofilm clinical relevance. Multifactorial features such as biocompatibility of embedded compounds, receptor site characteristics, and drug delivery efficiency have been found to influence the bioactive glass capability of acting both as an anti-biofilm agent and as a bone repairing biomaterial. Accordingly, further in vitro and in vivo studies are required to select the most promising anti-biofilm agents which should be incorporated into bioactive glasses to counteract biofilm proliferation, without inducing toxic effects on human cells, and with the added functionality of promoting bone regeneration. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 672-679, 2017.
Asunto(s)
Antiinfecciosos , Biopelículas/efectos de los fármacos , Regeneración Ósea/efectos de los fármacos , Sustitutos de Huesos , Cerámica , Materiales Biocompatibles Revestidos , Animales , Antiinfecciosos/química , Antiinfecciosos/uso terapéutico , Biopelículas/crecimiento & desarrollo , Sustitutos de Huesos/química , Sustitutos de Huesos/uso terapéutico , Cerámica/química , Cerámica/uso terapéutico , Materiales Biocompatibles Revestidos/química , Materiales Biocompatibles Revestidos/uso terapéutico , HumanosRESUMEN
In this work, TiO2 ceramic scaffolds were fabricated by the replica method using polyurethane (PU) sponges. Suspensions with high solid content were used to achieve scaffolds with improved mechanical behavior. TiO2 ceramic suspensions were optimized by rheological studies using different additives. It was found that the composition with 0.5 wt % Darvan enhanced the covering of the sponge struts. PU sponges of 45 to 80 ppi (pore per inch) were well coated without clogging pores. A thermal treatment with varying holding times, temperatures and heating rates was adjusted. The influence of different pore sizes on mechanical strength was evaluated. It was possible to obtain TiO2 scaffolds with 90% porosity and high pore interconnectivity, having compressive strength exceeding 0.6 MPa. TiO2 scaffolds were filled up with a 58S bioactive glass suspension to impart bioactive character to the scaffolds. These hybrid structures presented mechanical strengthening of about 26-213% depending on their sponge porosity. The prediction for cells viability via zeta potential measures indicated that this hybrid material is very promising for scaffold application with -19 to -25 mV between pH of 7.35-7.45. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 590-600, 2017.