RESUMEN
Behavioral disturbances are observed in most patients suffering from diabetes. According to some evidence, pro-inflammatory cytokines have a key role both in diabetes and behavioral disorders, such as anxiety and depression. In this study, the effect of chronic administration of loganin, as a bioflavonoid, was investigated on pro-inflammatory cytokines and depression- and anxiety-like behaviors in streptozotocin-induced diabetes in male Wistar rats. Blood levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were assessed by enzyme-linked immunosorbent assay method. Depression- and anxiety-like behaviors were evaluated by forced swimming test (FST), elevated plus maze (EPM), and open field test (OFT), respectively. Body weight was also measured before the interventions and after the experiments in all groups. Our findings show that loganin-treated animals had significantly lower serum concentrations of IL-6 and TNF-α compared with the diabetic group. In the EPM test, loganin treatment significantly increased the percentage of the open arm time and open arm entries. Moreover, loganin treatment significantly decreased the grooming time and restored distance traveled and center crossing in the OFT. However, it decreased immobility time in the FST. Loganin treatment also significantly restored body weight gain and attenuated blood glucose changes in the diabetic rats. These results indicate that loganin possibly alleviates depression- and anxiety-like behaviors associated with diabetes through lowering the blood glucose and pro-inflammatory cytokine levels. More research is required to show the exact mechanism of antidepressant and anxiolytic effects of loganin in diabetes.
Asunto(s)
Conducta Animal/efectos de los fármacos , Diabetes Mellitus Experimental/psicología , Iridoides/farmacología , Animales , Ansiedad/etiología , Citocinas/sangre , Depresión/etiología , Diabetes Mellitus Experimental/sangre , Interleucina-6/sangre , Masculino , Ratas , Ratas Wistar , Factor de Necrosis Tumoral alfa/sangreRESUMEN
Behavioral disturbances are observed in most patients suffering from diabetes. According to some evidence, pro-inflammatory cytokines have a key role both in diabetes and behavioral disorders, such as anxiety and depression. In this study, the effect of chronic administration of loganin, as a bioflavonoid, was investigated on pro-inflammatory cytokines and depression- and anxiety-like behaviors in streptozotocin-induced diabetes in male Wistar rats. Blood levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were assessed by enzyme-linked immunosorbent assay method. Depression- and anxiety-like behaviors were evaluated by forced swimming test (FST), elevated plus maze (EPM), and open field test (OFT), respectively. Body weight was also measured before the interventions and after the experiments in all groups. Our findings show that loganin-treated animals had significantly lower serum concentrations of IL-6 and TNF-α compared with the diabetic group. In the EPM test, loganin treatment significantly increased the percentage of the open arm time and open arm entries. Moreover, loganin treatment significantly decreased the grooming time and restored distance traveled and center crossing in the OFT. However, it decreased immobility time in the FST. Loganin treatment also significantly restored body weight gain and attenuated blood glucose changes in the diabetic rats. These results indicate that loganin possibly alleviates depression- and anxiety-like behaviors associated with diabetes through lowering the blood glucose and pro-inflammatory cytokine levels. More research is required to show the exact mechanism of antidepressant and anxiolytic effects of loganin in diabetes.
Asunto(s)
Conducta Animal/efectos de los fármacos , Citocinas/sangre , Diabetes Mellitus Experimental , Iridoides/farmacología , Animales , Ansiedad/etiología , Depresión/etiología , Masculino , Ratas , Ratas WistarRESUMEN
Prolonged neuronal depression after spreading depression (SD) is followed by a late cellular and synaptic hyperexcitability. Intra- and extracellular recordings of bioelectrical activities were performed in the rodent hippocampus to investigate the role of γ-aminobutyric acid (GABA)-mediated inhibition in the late hyperexcitable state of SD. The effect of KCl-induced negative DC potential shifts was investigated on extracellularly recorded paired-pulse depression (PPD) and bicuculline-induced afterdischarges as well as intracellularly recorded inhibitory post synaptic potentials (IPSPs) in the hippocampal CA1 area. The results revealed that SD decreased the degree of PPD, enhanced the number and duration of bicuculline-induced afterdischarges, and reduced the amplitude and duration of IPSPs. Application of low concentrations of bicuculline before the induction of SD enhanced the inhibitory effect of SD on IPSPs. Data indicate the contribution of GABA-mediated inhibition to SD-induced delayed hyperexcitability. Modulation of GABA function in the late hyperexcitability phase of SD may play a role in therapeutic management of SD-related neurological disorders.
Asunto(s)
Región CA1 Hipocampal/fisiología , Depresión de Propagación Cortical/fisiología , Inhibición Neural/fisiología , Neuronas/fisiología , Animales , Bicuculina/farmacología , Región CA1 Hipocampal/efectos de los fármacos , Depresión de Propagación Cortical/efectos de los fármacos , Antagonistas de Receptores de GABA-A/farmacología , Potenciales Postsinápticos Inhibidores/efectos de los fármacos , Potenciales Postsinápticos Inhibidores/fisiología , Masculino , Microelectrodos , Inhibición Neural/efectos de los fármacos , Neuronas/efectos de los fármacos , Cloruro de Potasio , Ratas Wistar , Ácido gamma-Aminobutírico/metabolismoRESUMEN
BACKGROUND: Oxidative stress is the main mechanism resulting in cyclosporine-induced nephrotoxicity. Because of its ability to stimulate endogenous melatonin production, isoproterenol is one of the most powerful antioxidant drugs. In this study, we sought to determine the effect of isoproterenol on cyclosporine-induced nephrotoxicity in rats. MATERIALS AND METHODS: Thirty two young male Wistar rats were divided into four groups: of group A were controls that received placebo; group B, received intraperitoneal isoproterenol (20 mg/kg/d) alone; group C, intravenous cyclosporine (15 mg/kg/d) alone; and group D, both drugs simultaneously at the same doses and durations namely cyclosporine 1 week after administration of isoproterenol. Blood samples to measure serum urea, creatinine, and melatonin levels were drawn four times for each group: before injection, at the mid period of treatment, at the end of treatment, and 1 week after the last injections. RESULTS: Isoproterenol increased mean serum melatonin level in groups B and D rats (P < .05). With regard to deteriorated renal function [DRF = (urea + creatinine)/2], administration of cyclosporine with (group D) or without (group C) isoproterenol was associated with decreased renal function (P < .05), although it was more perturbed in the latter instance. Measured DRF at the middle and the end of drug administration periods of A and B (revealed significant differences compared with groups C and D; P < .05). DISCUSSION: Although cyclosporine-induced nephrotoxicity is not completely eliminated by isoproterenol, the latter showed some protective effects.
Asunto(s)
Ciclosporina/toxicidad , Isoproterenol/uso terapéutico , Riñón/patología , Estrés Oxidativo/efectos de los fármacos , Animales , Antioxidantes/metabolismo , Creatinina/sangre , Riñón/efectos de los fármacos , Masculino , Melatonina/sangre , Ratas , Ratas Wistar , Urea/sangreRESUMEN
To investigate the effects of nibbling and gorging dietary regimens on weight and lipid profiles in rat, thirty female Wistar rats, after 10 day acclimatization period, were weighed and randomly assigned into two equal groups. They were fed the same food for 60 days as eight meals at 2 h intervals starting from 6 pm (nibbling group) or as two meals at 9 pm and 6 am (gorging group). The serum lipid levels and weight of animals were determined before and after the intervention. The body weight in two groups increased significantly (p < 0.001) during the period of study but there was no significant (p > 0.05) difference between two groups before and after the intervention. Nibbling regimen caused a reduction in the serum Total Cholesterol (TC), triglyceride and LDL-C levels, whereas these parameters increased during gorging diet. However, none of these changes were significant. There was a significant decrease (p < 0.05) in TC and LDL-C levels in nibbling diet compared to gorging one. According to obtained results, nibbling regimen has better effect on lipid profile than gorging one in rat.