RESUMEN
Transporter-mediated drug-drug interactions (DDIs) are a major cause of drug toxicities. Using published genome-wide association studies (GWAS) of the human metabolome, we identified 20 metabolites associated with genetic variants in organic anion transporter, OATP1B1 (P < 5 × 10-8 ). Of these, 12 metabolites were significantly higher in plasma samples from volunteers dosed with the OATP1B1 inhibitor, cyclosporine (CSA) vs. placebo (q-value < 0.2). Conjugated bile acids and fatty acid dicarboxylates were among the metabolites discovered using both GWAS and CSA administration. In vitro studies confirmed tetradecanedioate (TDA) and hexadecanedioate (HDA) were novel substrates of OATP1B1 as well as OAT1 and OAT3. This study highlights the use of multiple datasets for the discovery of endogenous metabolites that represent potential in vivo biomarkers for transporter-mediated DDIs. Future studies are needed to determine whether these metabolites can serve as qualified biomarkers for organic anion transporters. Quantitative relationships between metabolite levels and modulation of transporters should be established.
Asunto(s)
Ácidos y Sales Biliares/sangre , Ácidos Dicarboxílicos/sangre , Ácidos Grasos/sangre , Estudio de Asociación del Genoma Completo , Transportador 1 de Anión Orgánico Específico del Hígado/genética , Transportador 1 de Anión Orgánico Específico del Hígado/metabolismo , Metabolómica , Biomarcadores/metabolismo , Ciclosporina/farmacología , Interacciones Farmacológicas/genética , Células HEK293 , Humanos , Transportador 1 de Anión Orgánico Específico del Hígado/antagonistas & inhibidores , Miristatos/metabolismo , Proteína 1 de Transporte de Anión Orgánico/metabolismo , Transportadores de Anión Orgánico Sodio-Independiente/metabolismo , Ácidos Palmíticos/metabolismo , Pravastatina/farmacologíaRESUMEN
Nitronyl nitroxides (NN) effectively decompose free radicals (. As brain endothelium, forming the blood-brain barrier (BBB), is both the main source and the target of reactive species during cerebral oxidative stress, we studied the effect of NN on brain endothelial cells injured by the mediator of oxidative stress H(2)O(2) (. H(2)O(2) caused hydroxyl radical generation, lipid peroxidation, membrane dysfunction, membrane leak and cell death, concentration dependently. Due to 0.5 mM H(2)O(2), oxy-radical-induced membrane phospholipid peroxidation (malondialdehyde) increased to 0.61+/-0.04 nmol/mg protein vs control (0.32+/-0.03, p<0.05), cells lost cytosolic proteins into the medium and viability decreased to 28+/-2% of control (p<0.05). Permeability through the endothelial monolayer (measure for the tightness of the BBB) rose to 250+/-40% after 0.15 mM H(2)O(2) (p<0.001). Addition of 10 microM of the NN 5,5-dimethyl-2,4-diphenyl-4-methoxy-2-imidazoline-3-oxide-1-oxyl (NN-2), 1 mM phenylbutyl nitrone (PBN), or 10 microM of the lazaroid U83836E improved cell viability during incubation with 0.5 mM H(2)O(2) to 57+/-1%, 49+/-2%, and 42+/-3% (p<0.05, vs drug-free H(2)O(2) group). The permeability enhancement by 0.15 mM H(2)O(2) was reduced to 171+/-21%, 170+/-25%, and 118+/-32% (p<0.05 vs drug-free H(2)O(2) group). Generally, the assumption is supported that during cerebral oxidative stress the protection should also be directed to the cells of the BBB, which can be provided by antioxidative approaches. NN represent a new group of antioxdatively acting cytoprotectiva improving the survival and function of the endothelium against oxidative stress.
Asunto(s)
Barrera Hematoencefálica/fisiología , Cromanos/farmacología , Endotelio Vascular/efectos de los fármacos , Fármacos Neuroprotectores/farmacología , Óxido Nítrico/análisis , Estrés Oxidativo/efectos de los fármacos , Piperazinas/farmacología , Animales , Barrera Hematoencefálica/efectos de los fármacos , Encéfalo/citología , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Óxidos N-Cíclicos/farmacología , Relación Dosis-Respuesta a Droga , Espectroscopía de Resonancia por Spin del Electrón , Endotelio Vascular/fisiología , Fluoresceína , Depuradores de Radicales Libres/farmacología , Peróxido de Hidrógeno/metabolismo , Peróxido de Hidrógeno/farmacología , L-Lactato Deshidrogenasa/efectos de los fármacos , Malondialdehído/metabolismo , Óxido Nítrico/química , Óxido Nítrico/metabolismo , Óxidos de Nitrógeno/análisis , Óxidos de Nitrógeno/farmacología , RatasRESUMEN
The function of the blood-brain barrier (BBB) can be impaired by free radicals. Since free radicals have only a limited diffusion capacity, we tested the possibility whether one of the major secondary lipid peroxidation product - 4-hydroxynonenal, is able to influence the permeability of the BBB. Therefore, we established an in vitro BBB model and tested its capacity to degrade 4-hydroxynonenal. Although, endothelial cells and astrocytes, possess the ability to degrade 4-hydroxynonenal the aldehyde is able to increase the permeability of the BBB. Since aldehydic lipid peroxidation products are metabolized via conjugation with glutathione we proofed that a decrease in glutathione is also able to increase the permeability of the BBB. We concluded that 4-hydroxynonenal is able to impair the BBB function via the decrease of reduced glutathione.
Asunto(s)
Aldehídos/farmacocinética , Barrera Hematoencefálica/fisiología , Encéfalo/metabolismo , Permeabilidad de la Membrana Celular/fisiología , Inhibidores de Cisteína Proteinasa/farmacología , Endotelio Vascular/metabolismo , Estrés Oxidativo/fisiología , Animales , Astrocitos/efectos de los fármacos , Astrocitos/metabolismo , Barrera Hematoencefálica/efectos de los fármacos , Encéfalo/fisiopatología , Línea Celular Transformada/efectos de los fármacos , Línea Celular Transformada/metabolismo , Permeabilidad de la Membrana Celular/efectos de los fármacos , Endotelio Vascular/efectos de los fármacos , Radicales Libres/metabolismo , Glutatión/metabolismo , Peroxidación de Lípido/fisiología , Modelos Biológicos , Oxidación-Reducción/efectos de los fármacos , RatasRESUMEN
An organotypic culture system of the early postnatal rat retina was developed to study microglial activation within a tissue environment. One day after tissue preparation, microglial cells of the ganglion cell/nerve fiber layer revealed features of activation. Cells acquired an ameboid morphology as revealed by Bandeiraea simplicifolia lectin staining. Proliferation-as revealed by Ki67 immunocytochemistry-resulted in higher cell densities. In the supernatant, tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and monocyte chemoattractant factor-1 (MCP-1) were detected by using specific enzyme-linked immunosorbent assay systems, activated microglia being the most likely source of their release. After 6 days in vitro (div), microglial cells regained their resting morphology, and cell counts returned to control levels. Concomitantly, the release activity decreased to undetectable levels. When slices were treated at this later stage of cultivation (>6 div) with bacterial lipopolysaccharide (LPS; 100 ng/ml for 24 hours), microglial cells became activated, as revealed by a change in morphology. In parallel, the LPS treatment also resulted in high levels of TNF-alpha, IL-6, and MCP-1 in the culture medium. Both the release from the tissue and the morphological changes of the microglia were reversible. Seventy-two hours after LPS removal, only microglia with ramified morphology were found, and release activities returned to baseline. These data suggest that the organotypic culture of the retina is a useful model for studying microglial activation from its resting form.
Asunto(s)
Células Cultivadas/citología , Microglía/citología , Modelos Biológicos , Ratas Wistar/anatomía & histología , Retina/citología , Animales , Animales Recién Nacidos/anatomía & histología , Animales Recién Nacidos/crecimiento & desarrollo , Animales Recién Nacidos/metabolismo , Astrocitos/citología , Astrocitos/metabolismo , Capilares/citología , Capilares/metabolismo , Diferenciación Celular/efectos de los fármacos , Diferenciación Celular/fisiología , División Celular/efectos de los fármacos , División Celular/fisiología , Tamaño de la Célula/efectos de los fármacos , Tamaño de la Célula/fisiología , Células Cultivadas/efectos de los fármacos , Células Cultivadas/metabolismo , Quimiocinas/metabolismo , Citocinas/metabolismo , Antígeno Ki-67/metabolismo , Lipopolisacáridos/farmacología , Microglía/efectos de los fármacos , Microglía/metabolismo , Técnicas de Cultivo de Órganos , Ratas , Ratas Wistar/crecimiento & desarrollo , Ratas Wistar/metabolismo , Retina/efectos de los fármacos , Retina/metabolismo , Células Ganglionares de la Retina/citología , Células Ganglionares de la Retina/metabolismo , Factores de TiempoRESUMEN
Astrocytes (AC) induce blood-brain barrier (BBB) properties in brain endothelial cells (EC). As antioxidative activity (AOA) is assumed to be a BBB characteristic, we tested whether AC improve AOA of EC. Monocultivated AC showed higher AOA [manganese superoxide dismutase (SOD), catalase (Cat), glutathione peroxidase (GPx)] than EC. Cocultivation elevated AOA in EC (MnSOD, CuZnSOD, Cat, GPx), and AC (MnSOD, CuZnSOD, GPx). Hypoxia increased radical-induced membrane lipid peroxidation in monocultivated, but not in cocultivated EC. Thus, EC/AC cocultivation intensifies AOA in both cell types, protects the EC, and therefore, the BBB against oxidative stress. The high AOA is regarded as an essential property of the BBB, which is induced by AC.
Asunto(s)
Astrocitos/fisiología , Barrera Hematoencefálica , Endotelio Vascular/fisiología , Animales , Capilares/citología , Catalasa/metabolismo , Línea Celular , Endotelio Vascular/citología , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Peroxidación de Lípido , Malondialdehído/metabolismo , Ratas , Especies Reactivas de Oxígeno/metabolismo , Superóxido Dismutasa/metabolismoRESUMEN
There is contradictory information on the relevance of nitric oxide (NO) and cGMP for the function of brain capillary endothelial cells (BCEC) forming the blood-brain barrier (BBB). Therefore, NO/cGMP-mediated signal transduction was investigated in cell cultures of BCEC and of astrocytes (AC) inducing BBB properties in BCEC. Constitutive, Ca2+-activated isoforms of NO synthase (NOS) were found in BCEC (endothelial NOS: eNOS) and in AC (neuronal NOS: nNOS), leading to increased NO release after incubation with the Ca2+-ionophore A23187. Both cell types expressed inducible NOS (iNOS) after incubation with cytokines. Soluble guanylate cyclase (sGC) was detected in both cell types. NO-dependent cGMP formation were observed in BCEC and, less pronounced, in AC. Furthermore, both cell types formed cGMP independently of NO via stimulation of particulate guanylate cyclase (pGC). cGMP-dependent protein kinase (PKG) type Ibeta, but not type II, was expressed in BCEC and AC. In BCEC, vasodilator-stimulated phosphoprotein (VASP) was detected, an established substrate of PKG and associated with microfilaments and cell-cell contacts. Phosphorylation of VASP was intensified by increased intracellular cGMP concentrations. The results indicate that BCEC and, to a smaller degree, AC can form NO and cGMP in response to different stimuli. In BCEC, NO/cGMP-dependent phosphorylation of VASP is demonstrated, thus providing a possibility of influencing cell-cell contacts.
Asunto(s)
Barrera Hematoencefálica/fisiología , Moléculas de Adhesión Celular/metabolismo , GMP Cíclico/metabolismo , Endotelio Vascular/enzimología , Óxido Nítrico/metabolismo , Fosfoproteínas/metabolismo , Animales , Astrocitos/química , Astrocitos/citología , Astrocitos/enzimología , Proteínas Sanguíneas/metabolismo , Capilares/química , Capilares/citología , Capilares/enzimología , Moléculas de Adhesión Celular/análisis , Comunicación Celular/fisiología , Células Cultivadas , GMP Cíclico/análisis , Proteína Quinasa Dependiente de GMP Cíclico Tipo I , Proteínas Quinasas Dependientes de GMP Cíclico/genética , Proteínas Quinasas Dependientes de GMP Cíclico/metabolismo , Endotelio Vascular/química , Endotelio Vascular/citología , Regulación Enzimológica de la Expresión Génica , Guanilato Ciclasa/genética , Guanilato Ciclasa/metabolismo , Proteínas de Microfilamentos , Nitratos/análisis , Óxido Nítrico Sintasa/genética , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico Sintasa de Tipo II , Nitritos/análisis , Fosfoproteínas/análisis , Fosforilación , ARN Mensajero/análisis , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Transducción de Señal/fisiologíaRESUMEN
Little is known about pharmacological interventions with thiophosphates or lazaroids in endothelial cells injured by hypoxia/reoxygenation with respect to membrane lipid peroxidation (LPO) caused by reactive oxygen species. Therefore, a cell line of bovine aortic endothelial cells was studied after 120-min hypoxia followed by 30-min reoxygenation, resulting in moderate and predominantly reversible injury (energy depression/cytosolic Ca2+-accumulation during hypoxia, which almost normalized during reoxygenation; membrane blebs, an increasing amount of lysosomes, vacuolization, lipofuscin formation, alterations in mitochondria size, some lyzed cells). 18.9 +/- 4.3% of the cells died. Radical-induced LPO measured as malondialdehyde continuously increased to 2.18 +/- 0.17 nmol/mg of protein after reoxygenation vs control (0.41 +/- 0.13, P < 0.05). Simultaneously, the content of 4-hydroxynonenal, a novel indicator of LPO, increased from 0.02 +/- 0.01 to 0.11 +/- 0.02 nmol/mg of protein (P < 0.01). The results support the assumption that reoxygenation injury is accompanied by an increase in membrane LPO, causing structural and functional disturbances in the monolayer. The thiophosphate WR 2721 [S-2-(3-aminopropylamino) ethylphosphorothioic acid] and the lazaroid U83836E [(-)-2-[[4-(2,6-di-1-pyrrolidinyl-4-pyrimidinyl)-1-piperazinyl] methyl]-3,4-dihydro-2,5,7,8-tetramethyl-2H-1-benzopyran-6-ol (dihydrochloride)] were effective scavengers of .OH, being more efficient than trolox C (6-hydroxy-2,5,7,8-tetramethylchroman-2-carbon acid) used as standard (EC50: 12, 5 and 15 microM, respectively, measured by electron spin resonance spectroscopy). One mM WR 2721, 10 microM U83836E, and 5 microM trolox C reduced formation of malondialdehyde during hypoxia/reoxygenation to 53 +/- 7, 51 +/- 10 and 48 +/- 6%, respectively (P < 0.05 each, versus control). In general, WR 2721 and U83836E prevent radical-induced membrane LPO in a model of endothelial cells injured by hypoxia/reoxygenation. The use of these two agents is a new approach to protect the endothelium against oxidative stress.
Asunto(s)
Amifostina/farmacología , Cromanos/farmacología , Citoprotección , Endotelio Vascular/efectos de los fármacos , Depuradores de Radicales Libres/farmacología , Peroxidación de Lípido/efectos de los fármacos , Piperazinas/farmacología , Animales , Antioxidantes/farmacología , Aorta/citología , Aorta/efectos de los fármacos , Bovinos , Hipoxia de la Célula/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Endotelio Vascular/citología , Oxígeno/farmacologíaRESUMEN
A cell culture model of blood-brain barrier (BBB, coculture of rat brain endothelial cells with rat astrocytes) was used to investigate the effect of nitric oxide (.NO) on the damage of the BBB induced by hypoxia/reoxygenation (H/R). Permeability coefficient of fluorescein across the endothelium was used as a marker of BBB tightness. The permeability coefficient increased 5.2 times after H/R indicating strong disruption of the BBB. The presence of the .NO donor S-nitroso-N-acetylpenicillamine (SNAP, 30 microM), authentic .NO (6 microM) or superoxide dismutase (50 units/ml) during H/R attenuated H/R-induced increase in permeability. 30 microM SNAP or 6 microM .NO did not influence the function of BBB during normoxia, however, severe disruption was observed using 150 microM of SNAP and more than 24 microM of .NO. After H/R of endothelial cells, the content of malondialdehyde (MDA) increased 2.3 times indicating radical-induced peroxidation of membrane lipids. 30 microM SNAP or 6 microM authentic .NO completely prevented MDA formation. The results show that .NO may effectively scavenge reactive oxygen species formed during H/R of brain capillary endothelial cells, affording protection of BBB at the molecular and functional level.
Asunto(s)
Barrera Hematoencefálica/fisiología , Hipoxia/metabolismo , Óxido Nítrico/fisiología , Animales , Barrera Hematoencefálica/efectos de los fármacos , Encéfalo/citología , Permeabilidad de la Membrana Celular , Células Cultivadas , Endotelio/citología , Fluoresceína/farmacocinética , Guanilato Ciclasa/genética , Peroxidación de Lípido , Malondialdehído/metabolismo , Óxido Nítrico/farmacología , Compuestos Nitrosos/farmacología , Ratas , Especies Reactivas de Oxígeno/metabolismo , Daño por Reperfusión , Transducción de Señal , Superóxido Dismutasa/farmacologíaRESUMEN
In this work we studied the mechanism of nitric oxide (NO) release underlying the vasorelaxant and antiaggregant effect of 3,4-dihydrodiazete 1,2-dioxides (DD). Six derivatives were included in the investigations, namely, 3-bromo- and 3-chloro-3,4,4-trimethyl-DD (1a,b), 3-bromo- and 3-chloro-4-methyl-3,4-hexamethylene-DD (2a,b), 3,3,4,4-tetramethyl-DD (3), and 3-methyl-3,4-hexamethylene-DD (4), and their reactivity toward thiols was analyzed. The 3-bromo- and 3-chloro-DD derivatives were found to react with thiols; this reaction can lead to NO formation, DD 2a being the most reactive compound. 2-(Hydroxyamino)-2-methylbutan-3-one oxime (5a) and 2-hydroxy-2-methylbutan-3-one oxime (6) were the main products isolated from the reaction of 1a with cysteine. Reaction rates of DD with thiols were dependent upon pH and concentration of the reagents. Maximum rates of NO release corresponded to thiol concentrations in the range of 1 mM. Consistent with reaction kinetics data and products isolated, a reaction mechanism was proposed. Addition of 2a to bovine aortic endothelial cells led to strong NO release indicating a reaction with endogenous thiols. In rat mesenterial arteries, the vasorelaxant action of 2a was only slightly influenced by addition of thiol to the incubation medium. For the most reactive DD derivatives, cytotoxic effects were observed at concentrations roughly 2 orders of magnitude higher than those inducing vasorelaxation.
Asunto(s)
Óxido Nítrico/química , Compuestos de Sulfhidrilo/química , Vasodilatadores/síntesis química , Animales , Óxidos N-Cíclicos/síntesis química , Óxidos N-Cíclicos/química , Óxidos N-Cíclicos/farmacología , Arterias Mesentéricas , Ratas , Vasodilatación , Vasodilatadores/química , Vasodilatadores/farmacologíaAsunto(s)
Astrocitos/efectos de los fármacos , Citocinas/farmacología , Peroxidación de Lípido/efectos de los fármacos , Óxido Nítrico/metabolismo , Animales , Astrocitos/patología , Barrera Hematoencefálica/efectos de los fármacos , Hipoxia de la Célula , Células Cultivadas , Inducción Enzimática/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , Interleucina-1/farmacología , Óxido Nítrico Sintasa/biosíntesis , Penicilamina/análogos & derivados , Penicilamina/farmacología , S-Nitroso-N-Acetilpenicilamina , Sustancias Reactivas al Ácido Tiobarbitúrico/análisis , Factor de Necrosis Tumoral alfa/farmacologíaRESUMEN
Spin trapping compounds are used frequently to detect free radicals released by cells. Their cytotoxicity has to be considered in order to prevent perturbations of normal cell growth and viability. Eleven spin traps (eight nitrones and three nitroso traps) have been tested for their effects on bovine aortic endothelial cells (toxicity range, 50% survival rate). The lowest cytotoxicity was found for 5,5-dimethylpyrroline-1-oxide and 2,2,4-trimethyl-2H-imidazole-1-oxide whereas nitrosobenzene and 2-methyl-2-nitrosopropane exerted the strongest cytotoxic effects. In addition, three nitronyl nitroxides were tested. Their cytotoxicity was found to be dependent on substitution, and the toxic concentration of a lipophilic derivative was found to be more than two orders lower as compared to a hydrophilic derivative. The results of this study indicate that most spin traps can be used in cell cultures at customary (i.e. millimolar) concentrations; caution is recommended when nitroso spin traps are applied to cells.
Asunto(s)
Supervivencia Celular/efectos de los fármacos , Óxidos N-Cíclicos/toxicidad , Endotelio Vascular/efectos de los fármacos , Óxidos de Nitrógeno/toxicidad , Compuestos Nitrosos/toxicidad , Marcadores de Spin , Animales , Aorta , Bovinos , Células Cultivadas , Endotelio Vascular/citología , Endotelio Vascular/patología , Estructura Molecular , Relación Estructura-ActividadRESUMEN
Nitronyl nitroxides (NN), a class of compounds which react with nitric oxide forming imino nitroxides, were applied in different systems for the detection of nitric oxide. Addition of a NN to planar monolayers of bovine aortic endothelial cells (BAEC) activated by Ca2+ ionophore A23187 immediately resulted in a strong decrease of the ozone-mediated .NO chemiluminescence. Simultaneously, a rapid diminution of the electron spin resonance (ESR) signal intensity of the NN (without detectable formation of the corresponding imino nitroxide) was observed; superoxide dismutase partially inhibited this decrease in the NN concentration. Model experiments using hypoxanthine/xanthine oxidase in aqueous solution and KO2 in dimethylsulfoxide as sources of O2.- revealed that there is a rapid reduction of nitronyl nitroxides by superoxide. The second order rate constant for the reaction of the water soluble NN with O2.- was determined to be 8.8 x 10(5) M-1s-1, which is more than two orders of magnitude higher than the value reported previously for reaction with .NO (Woldman et al., BBRC 202, 195-203, 1994). Reduction of the nitronyl nitroxide was also observed in the presence of glutathione, ascorbic acid or rabbit liver microsomes. Incorporation of both nitronyl and imino nitroxides into liposomes strongly decreased reduction by superoxide and other reductants, however, in the presence of microsomes, there was no protective effect by liposomal encapsulation of NN. The results indicate that in biological systems (in addition to other reducing agents) the presence of superoxide can prevent the detection of nitric oxide using nitronyl nitroxides.
Asunto(s)
Óxidos N-Cíclicos/química , Imidazoles/química , Óxido Nítrico/análisis , Superóxidos/química , Animales , Calcimicina/farmacología , Bovinos , Células Cultivadas , Espectroscopía de Resonancia por Spin del Electrón , Endotelio Vascular/efectos de los fármacos , Endotelio Vascular/metabolismo , Cinética , Liposomas/química , Mediciones Luminiscentes , Microsomas Hepáticos/química , Microsomas Hepáticos/metabolismo , NAD/metabolismo , Óxido Nítrico/química , Óxido Nítrico/metabolismo , Conejos , Superóxido Dismutasa/metabolismoRESUMEN
We measured the accumulation of 4-hydroxynonenal (HNE), a major lipid peroxidation product during hypoxia/reoxygenation of brain capillary endothelial cells (BCEC). The concentration of HNE after 2 h of hypoxia was 0.23 nmol/mg protein and rose up to 0.28 nmol/mg protein after 30 min of reoxygenation. That reflects a 1.5-fold increase, whereas aortic endothelial cells (AEC) increased the HNE level 5-fold, compared to the control. Therefore, the ability of BCEC to degrade exogenously added HNE was tested. The HNE consumption in BCEC achieved a rate of about 600 nmol.min-1.mg protein-1, about two times higher than in AEC. The higher ability of BCEC to degrade HNE is probably the reason of the 2-fold higher IC50 value against the aldehyde. Therefore, we concluded that the high ability of BCEC to degrade HNE is a substantial part of the secondary antioxidative defense of the brain.
Asunto(s)
Aldehídos/metabolismo , Encéfalo/metabolismo , Circulación Cerebrovascular/fisiología , Hipoxia/fisiopatología , Oxígeno/fisiología , Animales , Capilares/metabolismo , Bovinos , Células Cultivadas , Endotelio/metabolismoRESUMEN
The present study investigated the influence of MK-801 (N-methyl-D-aspartate receptor antagonist) and U83836E (antioxidative aminosteroid) on the permeability of sodium fluorescein through a cell barrier during hypoxia (2 h 95% N2/5% CO2). The barrier consisted of porcine brain capillary endothelial cells and of cerebral rat astrocytes cultivated on two sides of a filter. After hypoxia, the permeation of fluorescein was significantly increased (10.2 +/- 1.5 x 10(-3) cm/min, P < 0.001) compared to the normoxic control (2 h 95% O2/5% CO2, 1.8 +/- 0.6 x 10(-3) cm/min). The hypoxia-enhanced permeation was significantly (P < 0.05) reduced by 10 microM MK-801 (2.0 +/- 0.5 x 10(-3) cm/min) and 10 microM U83836E (3.1 +/- 1.3 x 10(-3) cm/min). The results demonstrate, for the first time in a cell culture system, that hypoxia impairs brain endothelial barrier function, and that this enhanced permeability can be influenced pharmacologically. It is concluded that two distinct pathogenic mechanisms are involved in hypoxic cerebral endothelial cell injury, and that cerebroprotection afforded by these agents may result, in part, from reductions in edema secondary to improved blood-brain barrier function.
Asunto(s)
Barrera Hematoencefálica/efectos de los fármacos , Circulación Cerebrovascular/efectos de los fármacos , Cromanos/farmacología , Maleato de Dizocilpina/farmacología , Endotelio Vascular/efectos de los fármacos , Hipoxia/metabolismo , Piperazinas/farmacología , Animales , Astrocitos/fisiología , Encéfalo/citología , Capilares , Células Cultivadas , Endotelio Vascular/metabolismo , Endotelio Vascular/patología , Fluoresceína , Fluoresceínas , Depuradores de Radicales Libres/farmacología , Hipoxia/patología , Ratas , Ratas Wistar , PorcinosRESUMEN
Reactive oxygen species are thought to be important for a variety of pathological processes in the brain. Endothelial cells have been proposed as both a significant source of oxidants and targets of oxidative damage. Therefore, lipid peroxidation (LPO) was investigated and compared to biochemical and morphological alterations in cultured pig brain capillary endothelial cells after hypoxia (120 min. 95% N2/5% CO2) and reoxygenation (30 min. 95% O2/5% CO2). The content of thiobarbituric acid reactive substances (TBARS) representing radical-induced LPO was 2.50 +/- 0.46 after hypoxia and 5.92 +/- 0.54 nmol/mg protein after reoxygenation (p < 0.05 each, vs. normoxic control 1.79 +/- 0.21). During hypoxia, ATP content decreased to 7.9 +/- 1.6 nmol/mg protein; lactate dehydrogenase activity in the incubation solution increased to 0.17 +/- 0.03 U/mg protein; (p < 0.05 vs. control 15.7 +/- 3.1 and 0.09 +/- 0.02, respectively). After hypoxia, morphological changes in lysosomes, multivesicular bodies and vacuoles were observed in contrast to normoxic cells. During reoxygenation, the ATP values were normalized; electron micrographs showed increasing amounts of lysosomes, multivesicular bodies, vacuoles, blebs and lipofuscin granula and lyzed cells. Comparing the biochemical and morphological observations, a sequence of disturbances occurred, in which energy depletion was accompanied and followed, respectively, by membrane destruction, cellular disintegration and an increase in LPO products. These results support the assumption that the damage of brain endothelial cells caused by hypoxia and reoxygenation is accompanied by peroxidation of membrane lipids.