RESUMEN

Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H(2)(18)O and 18O(2), and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.

Asunto(s)

Mononucleótido de Flavina/análogos & derivados , Mononucleótido de Flavina/biosíntesis , Mononucleótido de Flavina/metabolismo , Oxidorreductasas N-Desmetilantes/metabolismo , Sustitución de Aminoácidos , Clonación Molecular , Escherichia coli/enzimología , Escherichia coli/genética , Methylophilus methylotrophus/enzimología , Mutagénesis Sitio-Dirigida , Proteínas Recombinantes/metabolismo , Especificidad por Sustrato