RESUMEN

Cationic lanthanide complexes are generally able to spontaneously internalize into living cells. Following our previous works based on a diMe-cyclen framework, a second generation of cationic water-soluble lanthanide complexes based on a constrained cross-bridged cyclam macrocycle functionalized with donor-π-conjugated picolinate antennas was prepared with europium(III) and ytterbium(III). Their spectroscopic properties were thoroughly investigated in various solvents and rationalized with the help of DFT calculations. A significant improvement was observed in the case of the Eu3+ complex, while the Yb3+ analogue conserved photophysical properties in aqueous solvent. Two-photon (2P) microscopy imaging experiments on living T24 human cancer cells confirmed the spontaneous internalization of the probes and images with good signal-to-noise ratio were obtained in the classic NIR-to-visible configuration with the Eu3+ luminescent bioprobe and in the NIR-to-NIR with the Yb3+ one.

Asunto(s)

Complejos de Coordinación/química , Elementos de la Serie de los Lantanoides/química , Sustancias Luminiscentes/química , Compuestos Macrocíclicos/química , Imagen Óptica , Fotones , Cationes/química , Línea Celular Tumoral , Complejos de Coordinación/síntesis química , Teoría Funcional de la Densidad , Humanos , Sustancias Luminiscentes/síntesis química , Compuestos Macrocíclicos/síntesis química , Estructura Molecular , Espectroscopía Infrarroja Corta