RESUMEN

Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.

Asunto(s)

Anemia de Células Falciformes/patología , Eritrocitos/patología , Citometría de Flujo/métodos , Citometría de Flujo/instrumentación , Hemoglobina Falciforme/análisis , Humanos