RESUMEN
The paper presents the genetic characteristics of two strains of spring viraemia of carp virus (SVCV), Kirov/08 and Orenburg/14, isolated in the Kirov and Orenburg oblasts of the Russian Federation, respectively. Nucleotide sequence analysis of a 516-bp fragment of the G genes of Kirov/08 and Orenburg/14 showed 9.5% divergence. Phylogenetic analysis demonstrated a close relationship of these strains to SVCV isolates recovered in Russia, Ukraine, and the Republic of Moldova.
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Carpas , Enfermedades de los Peces , Infecciones por Rhabdoviridae , Rhabdoviridae , Animales , Filogenia , Rhabdoviridae/genética , Infecciones por Rhabdoviridae/veterinariaRESUMEN
With age, the incidence of cardiovascular diseases is steadily increasing worldwide. Living in the harsh climatic and geographical conditions of the Arctic is associated with premature aging, earlier and more frequent development of age-associated diseases. Apparently, these processes are caused by functional changes in large vessels, the state of which has not yet been studied. Aim and methods: to identify correlations of aging and biological age indicators with the parameters of central hemodynamics and arterial stiffness measured using applanation tonometry by the «Sphygmocor¼ device in middle-aged men living in the European North of Russia. For the first time, positive associations of parameters of peripheral and central blood pressure, augmentation index, and negative correlation of the time of return of the reflected wave and pulse pressure amplification with indicators of aging were noted, which indicates an age-related decrease in arterial elasticity. There was also a negative association of the subendocardial viability ratio with the northern experience and biological age. Theoretically considered, the results demonstrate the potential involvement of blood vessels in the pathogenesis of premature aging. The practical significance of the work is related to the need to develop preventive measures aimed at controlling blood pressure, reducing arterial stiffness and cardiovascular risk in middle-aged northerners.
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Rigidez Vascular , Envejecimiento , Presión Sanguínea , Hemodinámica , Humanos , Masculino , Persona de Mediana Edad , Federación de Rusia/epidemiologíaRESUMEN
BACKGROUND: The mechanisms of action and efficacy of cisplatin and paclitaxel at cell population level are well studied and documented, however the localized spatio-temporal effects of the drugs are less well understood. We explore the emergence of spatially preferential drug efficacy resulting from variations in mechanisms of cell-drug interactions. METHODS: 3D spheroids of HeLa-C3 cells were treated with drugs, cisplatin and paclitaxel. This was followed by sectioning and staining of the spheroids to track the spatio-temporal apoptotic effects of the drugs. A mechanistic drug-cell interaction model was developed and simulated to analyse the localized efficacy of these drugs. RESULTS: The outcomes of drug actions on a local cell population was dependant on the interactions between cell repair probability, intracellular drug concentration and cell's mitosis phase. In spheroids treated with cisplatin, drug induced apoptosis is found to be scattered throughout the volume of the spheroids. In contrast, effect of paclitaxel is found to be preferentially localized along the periphery of the spheroids. Combinatorial treatments of cisplatin and paclitaxel result in varying levels of cell apoptosis based on the scheduling strategy. CONCLUSIONS: The preferential action of paclitaxel can be attributed to the cell characteristics of the peripheral population. The model simulations and experimental data show that treatments initiated with paclitaxel are more efficacious due to the cascading of spatial effects of the drugs.
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Antineoplásicos/uso terapéutico , Imagenología Tridimensional/métodos , Neoplasias/diagnóstico por imagen , Neoplasias/tratamiento farmacológico , Esferoides Celulares/metabolismo , Apoptosis , Proliferación Celular , Femenino , Células HeLa , Humanos , TransfecciónRESUMEN
The first report of the new coronavirus infection (COVID-19) appeared at the end of December 2019 and in March,2020 the World Health Organization announced COVID-19 a pandemic. The steady number of newly detected cases increase predetermined the modernization of the global healthcare system, shifting the paradigm of fighting with the COVID-19 pandemic towards maximum resource conservation. The change in the medical care provision for infectious patient's approach led first to a gradual decrease, and then to a complete cessation of planned surgical treatment, outpatient examinations, as well as observation of high-risk patients, which primarily include elderly patients. As a result of this, the key objective of this systematic review was to analyze sources of existing practice of providing urological care to patients of the older age group in the context of the COVID-19 pandemic. In accordance with the search for the keywords, the study reflects world and its own experience of MSMSU Urology Department in the treatment of age-related patients in the current epidemiological situation. The analysis showed that infectious complications from the urinary system take a leading position among the common complications inherent in elderly patients with COVID-19. The development of catheter-associated infection, the occurrence of antibiotic-resistant forms of bacteria, asymptomatic bacteriuria are only a small part of the problems clinicians have to face in newly profiled departments.
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Betacoronavirus , Infecciones por Coronavirus , Pandemias , Neumonía Viral , Anciano , COVID-19 , Humanos , Neumonía Viral/epidemiología , SARS-CoV-2RESUMEN
The geochemical conditions of landscapes are the content in the environment of certain chemical elements and their compounds, the lack or excess of which causes deviations in the state of human health. This problem has arisen in connection with the extraction of alluvial diamonds and the forthcoming development of the Tomtor deposit of rare-earth metals in the territories where the indigenous peoples of the North live. The study included 107 indigenous people of the North, belonging to the ethnic group of Dolgans living in the village of Yuryung-Khaya, Anabar district, Yakutia of Russia. The method of mass spectrometry was used to study the content of 13 trace elements in blood serum (P, Sc, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Rb, Sr, Cs, Pb). The study revealed an increase in the content of the macroelement phosphorus (148 mg/L) and trace elements of chromium (277 µg/L), manganese (133 µg/L), iron (5219 µg/L), nickel (57 µg/L) in serum of Dolgans, which may affect the development of diseases of the cardiovascular system and other diseases among indigenous inhabitants of the Arctic under conditions of industrial development of territories.
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Exposición a Riesgos Ambientales/análisis , Etnicidad , Desarrollo Industrial , Oligoelementos/sangre , Adulto , Factores de Edad , Anciano , Regiones Árticas , Femenino , Humanos , Masculino , Persona de Mediana Edad , Factores Sexuales , Siberia , Adulto JovenRESUMEN
Staphylococcus aureus asymptomatically persists on the nasal mucosa, and also causes serious diseases in carriers (endogenous infection) and in patients in a hospital (nosocomial infection). Decolonization of nasal carriers of S. aureus is an important measure aimed at reducing the incidence of staphylococcal infections. Carriage is a form of nasal dysbiosis, therefore, the effectiveness of antibiotics for the decolonization of carriers, by definition, is low. The review discusses the prospects of using probiotics to restore the nasal microbiota. The commercial production of nasal probiotics has not yet been established, but developments in this direction are being carried out in different countries. The experimental substantiation of the possibility of using corynebacteria and other representatives of the nasal microbiota for the decolonization of staphylococcal carriers is presented, as well as the authors' ideas on how to improve the methods of microbial therapy. In particular, it was proposed to use biofilm probiotics, autoprobiotics, and autovaccines for this purpose.
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Portador Sano/microbiología , Probióticos/uso terapéutico , Infecciones Estafilocócicas/terapia , Antibacterianos/uso terapéutico , Infección Hospitalaria/microbiología , Infección Hospitalaria/prevención & control , Humanos , Staphylococcus aureusRESUMEN
Morphological changes in bacterial biofilm structures arise from the fluid-structure interactions between the biofilm and the surrounding fluid. Depending on the magnitude of the force acting on the structure, the bacteria rearrange to attain an equilibrium shape or get washed away by the moving fluid. Understanding the dynamics behind the evolution of such equilibrium or failed states can aid in development of tools for biofilm removal or eradication. We develop a Glazier-Graner-Hogeweg method-based model to explore the collective evolution of biofilm morphology arising from cell-cell and cell-fluid interactions. We show that low adherence and high motility of the cells leads to sloughing of biofilms. Also, streamers are found to form under laminar flow conditions in tightly packed biofilms. In mixed species biofilms, we found that a species with less cell-cell binding affinity gets eroded faster than its counterpart. Therefore, we hypothesize that in nature these less-adherent species should be present encapsulated within the biofilm structure to maximize their chances of survival.
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Adhesión Bacteriana/fisiología , Biopelículas/crecimiento & desarrollo , Hidrodinámica , Estrés Mecánico , Simulación por Computador , Pseudomonas aeruginosa/fisiología , Resistencia al Corte/fisiologíaRESUMEN
An ointment containing metabolites of Bacillus sp. microorganisms isolated from permafrost samples was applied onto the skin wound of BALB/c mice. Metabolites isolated during culturing of Bacillus sp. at 37°C produced a potent therapeutic effect and promoted wound epithelialization by 30% in comparison with the control (ointment base) and by 20% in comparison with Solcoseryl. Treatment with Bacillus sp. metabolites stimulated predominantly humoral immunity, reduced the time of wound contraction and the volume of scar tissue, and promoted complete hair recovery. These metabolites can be considered as modulators of the wound process with predominance of regeneration mechanisms.
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Bacillus/metabolismo , Hielos Perennes/microbiología , Animales , Inmunidad Humoral/efectos de los fármacos , Factores Inmunológicos/química , Factores Inmunológicos/farmacología , Masculino , Ratones , Ratones Endogámicos BALB C , TemperaturaRESUMEN
Four peptide sequences characterized by high content of hydrophobic, charged, and polar amino acids were obtained from 23 clones of M13 phage. Peptides P2 and P4 exhibited highest binding affinity for immobilized trypomastigotes. The inhibitory effects of peptides seemed to be due to blockade of certain epitopes on T. cruzi surface proteins responsible for interactions with the respective receptors of host cells.
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Estadios del Ciclo de Vida/efectos de los fármacos , Oligopéptidos/farmacología , Biblioteca de Péptidos , Tripanocidas/farmacología , Trypanosoma cruzi/efectos de los fármacos , Secuencia de Aminoácidos , Sitios de Unión , Biotinilación , Células HeLa , Humanos , Estadios del Ciclo de Vida/fisiología , Oligopéptidos/metabolismo , Unión Proteica , Tripanocidas/metabolismo , Trypanosoma cruzi/crecimiento & desarrollo , Trypanosoma cruzi/metabolismoRESUMEN
The majority of the available works have studied distinct hypoxic responses of respiratory and cardiovascular systems. This study examines how these systems interact while responding to hypoxia and whether baseline metrics moderate reactions to a hypoxic challenge. Central hemodynamic, aortic wave reflection, and gas exchange parameters were measured in 27 trained young men before and after 10-min normobaric isocapnic hypoxia (10 % O2). Associations were assessed by correlation and multiple regression analyses. Hypoxic changes in the parameters of pulse wave analysis such as augmentation index (-114 %, p=0.007), pulse pressure amplification (+6 %, p=0.020), time to aortic reflection wave (+21 %, p<0.001) report on the increase in arterial distensibility. Specifically, initially compliant arteries blunt the positive cardiac chronotropic response to hypoxia and facilitate the myocardial workload. The degree of blood oxygen desaturation is directly correlated with both baseline values and hypoxic responses of aortic and peripheral blood pressures. The hypoxia-induced gain in ventilation (VE), while controlling for basal VE and heart rate (HR), is inversely associated with deltaHR and deltasystolic blood pressure. The study suggests that cardiovascular and respiratory systems mutually supplement each other when responding to hypoxic challenge.
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Presión Sanguínea/fisiología , Frecuencia Cardíaca/fisiología , Hemodinámica/fisiología , Hipoxia/fisiopatología , Mecánica Respiratoria/fisiología , Adolescente , Humanos , Hipoxia/diagnóstico , Masculino , Valor Predictivo de las Pruebas , Intercambio Gaseoso Pulmonar/fisiología , Análisis de la Onda del Pulso/métodos , Adulto JovenRESUMEN
The application of sodium cyanide (NaCN) to the carotid body receptors (CBR) (CBR stimulation) induces rapid blood hyperglycemia and an increase in brain glucose retention. The commissural nucleus tractus solitarius (cNTS) is an essential relay nucleus in this hyperglycemic reflex; it receives glutamatergic afferents (that also release brain derived neurotrophic factor, BDNF) from the nodose-petrosal ganglia that relays CBR information. Previous work showed that AMPA in NTS blocks hyperglycemia and brain glucose retention after CBR stimulation. In contrast, BDNF, which attenuates glutamatergic AMPA currents in NTS, enhances these glycemic responses. Here we investigated the combined effects of BDNF and AMPA (and their antagonists) in NTS on the glycemic responses to CBR stimulation. Microinjections of BDNF plus AMPA into the cNTS before CBR stimulation in anesthetized rats, induced blood hyperglycemia and an increase in brain arteriovenous (a-v) of blood glucose concentration difference, which we infer is due to increased brain glucose retention. By contrast, the microinjection of the TrkB antagonist K252a plus AMPA abolished the glycemic responses to CBR stimulation similar to what is observed after AMPA pretreatments. In BDNF plus AMPA microinjections preceding CBR stimulation, the number of c-fos immunoreactive cNTS neurons increased. In contrast, in the rats microinjected with K252a plus AMPA in NTS, before CBR stimulation, c-fos expression in cNTS decreased. The expression of AMPA receptors GluR2/3 did not change in any of the studied groups. These results indicate that BDNF in cNTS plays a key role in the modulation of the hyperglycemic reflex initiated by CBR stimulation.
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Factor Neurotrófico Derivado del Encéfalo/metabolismo , Cuerpo Carotídeo/efectos de los fármacos , Cuerpo Carotídeo/metabolismo , Hiperglucemia/metabolismo , Núcleo Solitario/metabolismo , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiónico/metabolismo , Animales , Factor Neurotrófico Derivado del Encéfalo/administración & dosificación , Ensayo de Inmunoadsorción Enzimática , Glucosa/metabolismo , Hiperglucemia/inducido químicamente , Hiperglucemia/patología , Inmunohistoquímica , Masculino , Microinyecciones , Neurotransmisores/farmacología , Proteínas Proto-Oncogénicas c-fos/metabolismo , Distribución Aleatoria , Ratas Wistar , Receptor trkB/agonistas , Receptor trkB/antagonistas & inhibidores , Receptor trkB/metabolismo , Receptores AMPA/agonistas , Receptores AMPA/antagonistas & inhibidores , Receptores AMPA/metabolismo , Cianuro de Sodio/farmacología , Núcleo Solitario/citología , Núcleo Solitario/efectos de los fármacos , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiónico/administración & dosificaciónRESUMEN
Centruroides tecomanus is a medically important scorpion of the state of Colima (Mexico). This communication reports the identification of venom components of this scorpion with biological activity over insects/crickets (Acheta domestica), crustaceans/fresh water shrimps (Cambarellus montezumae), and mammalians/mice (Mus musculus, strain CD1). It also describes the pharmacological effects on cell lines in culture (L5178Y cells, HeLa cells, HuTu cells and Jurkat E6-1 cells), as well as on several types of bacteria (see below). The soluble venom of this scorpion was fractionated by high-performance liquid chromatography (HPLC) and collected separately in twelve independent fractions collected over 60 min run (5 min time apart each other). The HPLC components of fraction VII were lethal to all three species used for assay. The IVth fraction had a toxic effect on freshwater shrimps. In this species, fractions VI, VII and VIII were all lethal. For crickets, fractions V and VI were toxic and fraction VII was lethal. In mouse, the lethal components were found in fraction VII, whereas fraction VIII was toxic, but not lethal, at the doses assayed. The molecular weight of peptides from the various group of fractions were identified by mass spectrometry determination. Components lethal to mice showed molecular weights from 7013 to 7487 Da. Two peptides were obtained in homogeneous form and shown to be lethal to the three species of animal used for assay. The soluble venom tested on L5178Y cell line survival was shown to be cytotoxic, at 10-100 µg/mL concentration, when compared to control murine splenocytes (p = 0.007). The soluble venom applied to Hela, Hutu and Jurkat cell lines did not show cytotoxic effects at these concentrations. On the contrary, it seems to have a proliferative effect. However the HPLC fractions I, III, VI and XII do have a cytotoxic effect on Jurkat E06-1 cells in culture at 200 µg/mL concentration. The antimicrobial activity of the venom fractions on Staphylococcus aureus (gram-positive), Escherichia coli, Pseudomonas aeruginosa y Salmonella spp (gram-negative) was measured, using the liquid inhibition growth system. The four strains of bacteria used were susceptible to fractions III and IV, affecting all four bacterial strains at concentrations below 5 µg/mL.
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Antibacterianos/aislamiento & purificación , Antineoplásicos/aislamiento & purificación , Apoptosis/efectos de los fármacos , Descubrimiento de Drogas , Insecticidas/aislamiento & purificación , Venenos de Escorpión/química , Secuencia de Aminoácidos , Animales , Antibacterianos/efectos adversos , Antibacterianos/química , Antibacterianos/farmacología , Antineoplásicos/efectos adversos , Antineoplásicos/química , Antineoplásicos/farmacología , Proteínas de Artrópodos/química , Proteínas de Artrópodos/aislamiento & purificación , Proteínas de Artrópodos/farmacología , Proteínas de Artrópodos/toxicidad , Astacoidea/efectos de los fármacos , Astacoidea/crecimiento & desarrollo , Línea Celular Tumoral , Células Cultivadas , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Gramnegativas/crecimiento & desarrollo , Gryllidae , Humanos , Inyecciones Intraperitoneales , Insecticidas/química , Insecticidas/farmacología , Insecticidas/toxicidad , México , Ratones , Pruebas de Sensibilidad Microbiana , Venenos de Escorpión/administración & dosificación , Venenos de Escorpión/toxicidad , Escorpiones/crecimiento & desarrollo , Bazo/citología , Bazo/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrolloRESUMEN
Empirical data on the behavior of radionuclides in aquatic ecosystems are needed for radioecological modeling, which is commonly used for predicting transfer of radionuclides, estimating doses, and assessing possible adverse effects on species and communities. Preliminary studies of radioecological parameters including distribution coefficients and concentration ratios, for (90)Sr and (137)Cs were not in full agreement with the default values used in the ERICA Tool and the RESRAD BIOTA codes. The unique radiation situation in the Techa River, which was contaminated by long-lived radionuclides ((90)Sr and (137)Cs) in the middle of the last century allows improved knowledge about these parameters for river systems. Therefore, the study was focused on the evaluation of radioecological parameters (distribution coefficients and concentration ratios for (90)Sr and (137)Cs) for the Techa River and the Miass River, which is assumed as a comparison waterbody. To achieve the aim the current contamination of biotic and abiotic components of the river ecosystems was studied; distribution coefficients for (90)Sr and (137)Cs were calculated; concentration ratios of (90)Sr and (137)Cs for three fish species (roach, perch and pike), gastropods and filamentous algae were evaluated. Study results were then compared with default values available for use in the well-known computer codes ERICA Tool and RESRAD BIOTA (when site-specific data are not available). We show that the concentration ratios of (137)Cs in whole fish bodies depend on the predominant type of nutrition (carnivores and phytophagous). The results presented here are useful in the context of improving of tools for assessing concentrations of radionuclides in biota, which could rely on a wider range of ecosystem information compared with the process limited the current versions of ERICA and RESRAD codes. Further, the concentration ratios of (90)Sr are species-specific and strongly dependent on Ca(2+) concentration in water. The universal characteristic allows us to combine the data of fish caught in the water with different mineralization by multiplying the concentration of Ca(2+). The concentration ratios for fishes were well-fitted by Generalized Logistic Distribution function (GLD). In conclusion, the GLD can be used for probabilistic modeling of the concentration ratios in freshwater fishes to improve the confidence in the modeling results. This is important in the context of risk assessment and regulatory.
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Radioisótopos de Cesio/análisis , Ríos/química , Radioisótopos de Estroncio/análisis , Contaminantes Radiactivos del Agua/análisis , Animales , Peces , Gastrópodos , Sedimentos Geológicos/análisis , Monitoreo de Radiación , Federación de Rusia , Algas MarinasRESUMEN
Glutamate, released from central terminals of glossopharyngeal nerve, is a major excitatory neurotransmitter of commissural nucleus tractus solitarii (cNTS) afferent terminals, and brain derived neurotrophic factor (BDNF) has been shown to attenuate glutamatergic AMPA currents in NTS neurons. To test the hypothesis that AMPA contributes to glucose regulation in vivo modulating the hyperglycemic reflex with brain glucose retention (BGR), we microinjected AMPA and NBQX (AMPA antagonist) into the cNTS before carotid chemoreceptor stimulation in anesthetized normal Wistar rats, while hyperglycemic reflex an brain glucose retention (BGR) were analyzed. To investigate the underlying mechanisms, GluR2/3 receptor and c-Fos protein expressions in cNTS neurons were determined. We showed that AMPA in the cNTS before CChr stimulation inhibited BGR observed in aCSF group. In contrast, NBQX in similar conditions, did not modify the effects on glucose variables observed in aCSF control group. These experiments suggest that glutamatergic pathways, via AMPA receptors, in the cNTS may play a role in glucose homeostasis.
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Encéfalo/metabolismo , Cuerpo Carotídeo/fisiología , Glucosa/metabolismo , Hipoxia/fisiopatología , Receptores de Glutamato/fisiología , Núcleo Solitario/fisiología , Animales , Masculino , Quinoxalinas/farmacología , Ratas , Ratas Wistar , Receptores AMPA/fisiología , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiónico/farmacologíaAsunto(s)
Infecciones Bacterianas/cirugía , Fascitis Necrotizante/cirugía , Gangrena de Fournier/cirugía , Escroto/cirugía , Antibacterianos/uso terapéutico , Infecciones Bacterianas/diagnóstico , Infecciones Bacterianas/tratamiento farmacológico , Infecciones Bacterianas/microbiología , Fascia/efectos de los fármacos , Fascia/microbiología , Fascia/patología , Fascitis Necrotizante/diagnóstico , Fascitis Necrotizante/tratamiento farmacológico , Fascitis Necrotizante/microbiología , Fasciotomía , Gangrena de Fournier/diagnóstico , Gangrena de Fournier/tratamiento farmacológico , Gangrena de Fournier/microbiología , Humanos , Masculino , Escroto/efectos de los fármacos , Escroto/microbiología , Escroto/patologíaRESUMEN
Effectiveness of saline suspension of Corynebacterium pseudodiphtheriticum containing spray was assessed in a 30-days chamber experiment modeling the effects of hyperthermia and polluted air on humans. Spray was targeted at eliminating Staphylococcus aureus from the nasal cavity of human subjects. Three of four volunteers became S. aureus carriers in the course of the experiment, and one was a chronic carrier of methicillin-resistant S. aureus (MRSA). Spray application eradicated S. aureus in three subjects and reduced its presence in the MRSA carrier. Results of the study suggest that C. pseudodiphtheriticum can be used for control of S. aureus in the nasal environment. However, further investigations are required.
RESUMEN
The immunobiological potential of a new microorganism species isolated from permafrost specimens (PMO strain 3M) collected from the Mammoth Mountain in Yakutia was studied in laboratory mice. PMO injected intraperitoneally in doses of 2500 to 50 × 10(6) microbial bodies caused characteristic dose-dependent effects on the structure and functions of the immune system (thymus and spleen indexes, functional activity of splenic macrophages, cellular and humoral immunity). Doses of PMO stimulating functional activities of both cellular and humoral immunity were detected.
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Adaptación Fisiológica/inmunología , Bacillus/inmunología , Sistema Inmunológico/fisiología , Microbiología del Suelo , Glándulas Suprarrenales/inmunología , Glándulas Suprarrenales/metabolismo , Animales , Bacillus/genética , Bacillus/aislamiento & purificación , Recuento de Células , Clima Frío , Inmunidad Celular , Inmunidad Humoral , Ratones , Ratones Endogámicos CBA , Datos de Secuencia Molecular , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Siberia , Bazo/citología , Bazo/inmunología , Bazo/metabolismo , Timo/inmunología , Timo/metabolismoRESUMEN
The aim of this randomized, double-blind clinical trial was to determine the effectiveness of systemic antibiotic application followed by either topical ionized solution (IS) or topical saline solution (placebo) as surgical site infection (SSI) prophylaxis in appendectomy for non-perforated appendicitis. Prophylactic antibiotic was administered pre-incision and either topical IS or placebo was applied pre-suturing to 529 patients, who were then monitored for 30 days. When topical IS was used, SSI relative risk was 0.739 (95% CI 0.537, 1.019) and the SSI prevention rate was 26.06% (CI -1.8%, 46.3%). The number needed to treat was 14.80 patients and the number needed to harm was 209 in order for SSI to be avoided. In conclusion, there was a trend for topical IS prophylaxis to reduce SSI frequency in patients undergoing appendectomy for non-perforated appendicitis, but the result was not statistically significant.
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Antibacterianos/uso terapéutico , Profilaxis Antibiótica , Soluciones/administración & dosificación , Soluciones/uso terapéutico , Infección de la Herida Quirúrgica/tratamiento farmacológico , Administración Tópica , Adolescente , Adulto , Anciano , Apendicectomía/efectos adversos , Apendicitis/cirugía , Bacterias/clasificación , Niño , Preescolar , Femenino , Humanos , Masculino , Persona de Mediana Edad , Infección de la Herida Quirúrgica/microbiología , Resultado del Tratamiento , Adulto JovenRESUMEN
The purpose of the present work was to inform about the first case of meningitis associated to the bacteria Alcaligenes xylosoxidans in a patient with HIV/AIDS. The patient was a 46-year-old male, with the antecedent of have been diagnosed for HIV/ AIDS, who attended in the Hospital Universitario de Colima, Mexico, with fever, shock and meningismus. The study of the cerebrospinal fluid showed pleocytosis, elevated protein levels and hypoglycorrhachia. The culture yielded the presence of Alcaligenes xylosoxidans with sensitivity to ciprofloxacin. After 14 days of treatment with this antibiotic, the patient showed neurologic improvement and was able to continue with his outpatient antiretroviral treatment. The present case shows the importance of the inclusion of this bacterium in the differential diagnosis of the neurological infections in HIV/AIDS patients and emphasizes the importance of considering the bacterial meningitis in this population.