RESUMEN
The hybridoma technology was used to produce monoclonal antibodies to human ceruloplasmin. The antibodies were found to be related to Ig G1. Using these monoclonal antibodies for PAP-ELISA of ceruloplasmin, it was possible to determine 10(-11) M of the protein. Monoclonal antibodies coupled to CNBr-Sepharose were used for the rapid one-stage purification of ceruloplasmin from human placental serum. Ceruloplasmin obtained by this method contained no type 2 copper normally detected in protein preparations by conventional methods.
Asunto(s)
Anticuerpos Monoclonales/biosíntesis , Ceruloplasmina/inmunología , Animales , Ceruloplasmina/análisis , Humanos , Técnicas para Inmunoenzimas , Ratones , Ratones Endogámicos BALB CRESUMEN
Immunization of BALB/c and C3H/A mice with T-independent antigens (Vi-antigen of Salmonella typhi and polyvinyl pyrrolidone PVP350) induces the appearance of both antibody-forming cells (AFC) and a sharp increase in the number of cells forming nonspecific immunoglobulins (nIFC). This effect is not related to mitogenic properties of the antigens. The number of nIFC formed after simultaneous injection of both T-independent antigens does not differ from that of nIFC formed during immunization with each of these antigens alone. Thus, there was no summation of nIFC number after injection of two T-dependent or one T-dependent and one T-independent antigens. The mechanisms of nIFC formation under the influence of T-dependent and T-independent antigens are discussed.
Asunto(s)
Células Productoras de Anticuerpos , Antígenos Bacterianos/inmunología , Inmunización , Inmunoglobulinas/biosíntesis , Polisacáridos Bacterianos , Povidona/inmunología , Animales , Femenino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C3HRESUMEN
The role of antigen-binding cells in the formation of antigen-dependent nonspecific immunoglobulin producers in the Mishell-Dutton system was investigated. It was shown that the main part of antigen-dependent nonspecific immunoglobulin producers arise from the cells bearing antigen-specific receptors (as well as antibody producers). The nature of antigen-specific receptors of the precursors of antibody-forming and antigen-dependent nonspecific immunoglobulin-forming cells is discussed.
Asunto(s)
Células Productoras de Anticuerpos/inmunología , Antígenos/inmunología , Inmunoglobulinas/biosíntesis , Animales , Femenino , Técnicas In Vitro , Ratones , Ratones Endogámicos C57BL , Receptores de Antígenos/inmunología , Bazo/inmunologíaRESUMEN
Mice injected with syngeneic cellulose-conjugated immunoglobulins (Ig) containing antibodies to sheep red blood cells (SRBC) develop a specific non-responsiveness to SRBC. Such animals demonstrate a sharp decrease not only in the formation of anti-SRBC antibody producers but also of the cells secreting antigen-dependent nonspecific Ig. The inhibition of both these processes is antigen-specific. It is suggested that inhibition of the cells forming antigen-dependent nonspecific Ig is due to suppression of either hypothetic inductors or precursors of these cells expressing an idiotype spectrum similar to that of anti-SRBC antibody producers.
Asunto(s)
Células Productoras de Anticuerpos/inmunología , Eritrocitos/inmunología , Inmunoglobulinas/inmunología , Animales , Antígenos/inmunología , RatonesRESUMEN
Mice injected with isologous cellulose-conjugated immunoglobulins containing antibodies to sheep red blood cells develop a specific unresponsiveness to the homologous antigen. It was shown that such animals demonstrate a sharp decrease not only in the number of antibody-forming cells but also in the number of cells secreting antigen-dependent non-specific immunoglobulins. The suppression of both processes is antigen-specific. It is suggested that the reduced appearance of cells forming antigen-dependent non-specific immunoglobulins is due to suppression either of hypothetic inductors or precursors of these cells, which resemble the precursors of antibody producers in their idiotypes. The presence of the common precursors of antibody- and antigen-dependent non-specific immunoglobulin-forming cells is an alternative possibility.
Asunto(s)
Células Productoras de Anticuerpos/inmunología , Eritrocitos/inmunología , Inmunoglobulinas/biosíntesis , Animales , Pollos , Idiotipos de Inmunoglobulinas/inmunología , Inmunoglobulinas/inmunología , Terapia de Inmunosupresión , Isoantígenos/inmunología , Ratones , Ratones Endogámicos , OvinosRESUMEN
BALB/c mice were immunized with sheep red blood cells (T-dependent antigen) or with Vi-antigen Salmonella typhi (T-independent antigen). The kinetics of the development of antibody-forming and nonspecific immunoglobulin-forming cells (AFC and nIFC) was examined. It was shown that both antigens induce an increase in the nIFC number. The time course of AFC and nIFC growth during the primary and secondary immune responses appeared to be similar. Simultaneous injection of both antigens resulted in the formation of nIFC in an amount which was equal to the total number of nIFC formed in response to the antigens injected alone. It is concluded that T-dependent and T-independent antigens induced the differentiation of varying populations of nIFC precursors.