RESUMEN
The intensity of free radical oxidation processes in vivo (a model of induced oxidative stress) was studied after a probe administration of the fruit extract of the axillary blueberry (Vaccinium axillare Nakai). \Four groups (n = 40) of white CBA line male mice weighing 20-25 g were involved in the experiment: (1) intact control; (2) introduction of a 0.9% sodium-chloride solution orally for 10 days, a dose of 10 mL/kg per day; (3) "cisplatin" group (animals received 0.9% sodium-chloride solution similarly to group 2; on the fifth day of the experiment, cisplatin was administered one time by intraperitoneal injection at a dose of 7.5 mg/kg); and (4) "cisplatin + blueberry" group (mice received orally an extract of axillary-blueberry fruits at a dose of 10 mL/kg per day for 10 days; on the fifth day of the experiment, cisplatin was administered one time by intraperitoneal injection at a dose of 7.5 mg/kg). The antioxidant activity of the axillary blueberry was studied by a method of chemiluminescence. The analysis of kinetic parameters of chemiluminescence of mouse-kidney homogenate demonstrated that, against the background of a single intraperitoneal injection of cisplatin, oxidative stress develops in animals, with its severity decreasing under the action of axillary blueberry-fruit extract. The axillary blueberry-fruit extract has pronounced antioxidant properties and can play a role in the treatment and prevention of disease associated with oxidative stress.
RESUMEN
Ten secondary metabolites including flavonoids (1-8), caffeic (9) and chlorogenic (10) acids were structurally identified from the extract of Sakhalin bilberry Vaccinium smallii leaves and studied in vitro as potential cancer-preventive agents. The results showed that compounds 1-10 inhibited EGF-induced neoplastic transformation of mouse JB6 Cl 41 P+ cells in soft agar with an inhibition concentration (INCC50) of 20-80 µm. Moreover, all these natural products were non-toxic against JB6 Cl 41 P+ cells up to a concentration of 200 µm.