RESUMEN
Strenuous exercise, such as running a marathon, is known to suppress mucosal immunity for up to 24 hr, which can increase the risk of developing an upper respiratory tract infection (URTI) and reduced performance capacity (Allgrove JE, Geneen L, Latif S, Gleeson M. Influence of a fed or fasted state on the s-IgA response to prolonged cycling in active men and women. Int J Sport Nutr Exerc Metab. 2009;19(3):209-221; Barrett B, Locken K, Maberry R, Schwamman J, Brown R, Bobula J, Stauffacher EA. The Wisconsin Upper Respiratory Symptom Survey (WURSS): a new research instrument for assessing the common cold. J Fam Pract. 2002;51(3):265; Carpenter KC, Breslin WL, Davidson T, Adams A, McFarlin BK. Baker's yeast beta glucan supplementation increases monocytes and cytokines post-exercise: implications for infection risk? Br J Nutr. 2012;1-9). While many dietary interventions have been used to combat postexercise immune suppression, most have been ineffective. The key purpose of this study was to determine if baker's yeast ß-glucan (BG) could positively affect the immune system of individuals undergoing intense exercise stress using two experiments. In the first (E1; N = 182 men and women), BG was compared to placebo supplementation for the incidence of URTI symptoms for 28 days postmarathon. In the second (E2; N = 60 men and women) changes in salivary immunoglobulin A (IgA) were evaluated after 50-min of strenuous cycling when participants had been supplemented for 10 days with either BG (250 mg/day) or placebo (rice flour). For E1, subjects reported URTI symptoms using a daily health log. For E2, saliva was collected prior to, immediately, and 2-hr postexercise using a salivette. Data for E1 and E2 were analyzed using separate analyses of variance (ANOVAs) with repeated measures (p < .05). In E1, BG was associated with a 37% reduction in the number of cold/flu symptom days postmarathon compared to placebo (p = .026). In E2, BG was associated with a 32% increase in salivary IgA (p = .048) at 2 hr after exercise compared to placebo. In summary, the present study demonstrates that BG may reduce URTI symptomatic days and improve mucosal immunity (salivary IgA) postexercise.
Asunto(s)
Resfriado Común/tratamiento farmacológico , Suplementos Dietéticos , Ejercicio Físico/fisiología , Inmunoglobulina A/metabolismo , Gripe Humana/tratamiento farmacológico , Estrés Fisiológico/inmunología , beta-Glucanos/uso terapéutico , Adolescente , Adulto , Análisis de Varianza , Ciclismo/fisiología , Resfriado Común/complicaciones , Resfriado Común/inmunología , Resfriado Común/metabolismo , Femenino , Humanos , Sistema Inmunológico/efectos de los fármacos , Inmunidad/efectos de los fármacos , Gripe Humana/complicaciones , Gripe Humana/inmunología , Gripe Humana/metabolismo , Masculino , Persona de Mediana Edad , Membrana Mucosa/efectos de los fármacos , Membrana Mucosa/inmunología , Membrana Mucosa/metabolismo , Esfuerzo Físico/fisiología , Carrera/fisiología , Saccharomyces cerevisiae/química , Saliva/inmunología , Saliva/metabolismo , Adulto Joven , beta-Glucanos/farmacologíaRESUMEN
INTRODUCTION/PURPOSE: The purpose of this study was to determine the affects of repeated bouts of exercise in the same day on circulating leukocyte number and NK cell activity (NKCA). METHODS: Ten males (18-25 yr of age) were chosen to complete four trials: two exercise bouts (TB), AM exercise (AM-EX), PM exercise (PM-EX), and control (CN) in a random, counter-balanced order. Exercise consisted of 1-h cycle ergometry, split into three, 20-min segments (5 min at 50% [V0](2peak) and 15 min at 70% [V0](2peak)), and was separated by a 4-h passive recovery. Blood samples were collected from a peripheral arm vein before, immediately, 2 h, and 24 h after the AM bout, and a second series was drawn for the PM bout. Leukocyte subpopulations were measured by manual counting using a hemacytometer and Wright-Giemsa-stained differential slides. NKCA was measured with a whole-blood 51Cr-release assay against K562 target cells. RESULTS Total leukocyte (13.2 +/- 1.2 x 109 L-1, P < 0.035) and neutrophil (7.6 +/- 0.7 x 109 L-1, P < 0.001) counts were significantly higher after two bouts of exercise than a single exercise bout completed in the morning (leukocyte: 7.8 +/- 0.7 x 109 L-1; neutrophil: 5.2 +/- 0.5 x 109 L-1) or afternoon (leukocyte: 9.9 +/- 0.8 x 109 L-1; neutrophil: 5.6 +/- 0.9 x 109 L-1). Lymphocyte counts were significantly greater (P < 0.001) after PM (4.2 +/- 0.5 x 109 L-1) than AM (3.7 +/- 0.4 x 109 L-1) exercise. NKCA was significantly greater (P < 0.001) after exercise in the afternoon (35.3 +/- 8.1%) than morning (27.2 +/- 5.9%). CONCLUSION: PM exercise produced a larger increase in NKCA than AM exercise, possibly indicative of an interaction between exercise and a diurnal effect. These data suggest two bouts of endurance exercise in 1 d produce an "additive effect" for total leukocyte and neutrophil counts. and to a lesser degree lymphocyte counts, but did not appear to impact shifts in NKCA.