RESUMEN
Two methods for precise cell location and relocation were developed at the Cell Image Analysis Laboratory, NASA, to increase the efficiency of the cell image acquisition process. The first method uses a Zeiss Zonax with a 10 micron scanning stage and automatically records cell coordinates on 5.25-inch floppy disks. The second method uses a Digital Positioning Device with a 10 micron scanning stage, and the cell coordinates displayed on light-emitting diodes (LEDs) are recorded manually. Because of its superior speed and automation, the Zonax was selected as the method of choice. By using a Zonax for off-line cell location and another Zonax for on-line cell relocation, more than three times the number of digitized images were collected in ten months than in the previous three years. Other beneficial applications of the cell location and relocation methods were realized in photomicrography, data base verification and cytopathology.
Asunto(s)
Técnicas Citológicas , Computadores , Técnicas Citológicas/instrumentación , Programas InformáticosRESUMEN
The supportive properties of polyurethane foam wheelchair cushions change significantly during the first hours of use. Thus, cushions which initially appear to optimally distribute the patient's weight may not do so after a break-in period. To solve this problem, a cushion prestressing protocol has been devised which breaks-in the foam cushion prior to determination of the patient's pressure distribution. It was found that the useful life of these cushions is adversely affected by heavy static loading and high temperatures, and if allowed to rest, polyurethane foam cushions recover a portion of their original load-bearing properties.