RESUMEN
Ovarian reserve is a reflection of the overall female reproductive potential. Vitamin D status has been suspected to influence fetal development and female fertility. As maternal diet during pregnancy can affect fetal development and future fertility, we hypothesised that periconceptional and gestational Vitamin D restriction could affect folliculogenesis and AMH secretion in the offspring. Nineteen sexually mature Welsh mountain ewes were randomly assigned to Vitamin D3 deficient (VDD, n = 10) and Vitamin D3 control (VDC, n = 9) diets from 17 days (d) before mating, up to 127-130 days of gestation, when fetal ovaries were collected (3 from VDC and 6 from VDD). Serum 25(OH)D3 concentrations were lower in VDD compared with VDC (p < 0.05). Relative to total follicle number, the percentage of primordial follicles was higher (p < 0.05), while the percentage of primary follicles was lower (p < 0.05) in VDD group compared with VDC group fetal ovaries. The integrated density value and percentage of affected area in TUNEL staining in VDD group did not vary from VDC group fetal ovaries (p > 0.05). Relative expression of AMH mRNA and AMH protein in VDD fetal ovaries were not statistically different compared with controls (p > 0.05). The relative expression of VDR mRNA were lower in VDD compared with VDC group fetal ovaries (p < 0.05). These data indicate that maternal Vitamin D dietary restriction is associated with ovarian tissue stemness and increased primordial follicle number but does not promote normal follicle recruitment or development in sheep fetal ovaries.
Asunto(s)
Hormona Antimülleriana , Colecalciferol , Folículo Ovárico , Animales , Femenino , Hormona Antimülleriana/metabolismo , Hormona Antimülleriana/sangre , Embarazo , Oveja Doméstica , Dieta/veterinaria , Deficiencia de Vitamina D/veterinaria , Ovinos , Ovario/metabolismoRESUMEN
BACKGROUND: Research has demonstrated that certain nutrients can reverse infertility. Aims: The present study, "Impact of Omega-3 fatty acids preconception intake on some fertility parameters of female rats and foetuses quality" was conducted to further investigate this subject. METHODS: Subjects were thirty-four female and twelve male adult Albino rats, weighing 172-181 g. Initially, 12 male rats were assigned to treatment groups 1, 2, 3, and 4, and 24 females were assigned to treatment groups 5, 6, 7, and 8. The treatment protocol was administered orally as follow: groups 1 and 5 (controls), 0.3 ml distilled water (as placebo); groups 2 and 6, 250 mg/kg Omega-3 fatty acids (O3FA); groups 3 and 7, 25 mg/kg cyclophosphamide (CPP, negative control); and groups 4 and 8, 25 mg/kg CPP + 250 mg/kg of O3FA for 28 days. Thereafter, they were cohabitated (1:2 male to female ratio) into mating groups 1 to 7 until mating was confirmed. Ten pregnant rats (5 of which were administered 500 mg/kg O3FA on days 15 and 16 of gestation) were used for abortifacient effect experiment. RESULTS: Number of implantation sites (IM), implantation index (IMI), percentage of pregnant females (PPF), life foetal number, foetal weight, foetal crown-rump length (FCRL), corpora luteal number (CLN) and fertility index (FI) in O3FA-treated female rats and their foetuses significantly increased (P<0.05) compared with negative control. No noticeable abortifacient effect occurred. CONCLUSION: Preconception intake of O3FA may have impacted female fertility positively.